An Electrochemical DNA Microbiosensor Based on Succinimide-modified Acrylic Microspheres

Overview

Journal Sensors (Basel)

Publisher MDPI

Specialty Biotechnology

Date 2012 Jul 11

PMID 22778594

Citations 12

Authors

Alizar Ulianas

Lee Yook Heng

Sharina Abu Hanifah

Tan Ling Ling

Affiliations

Soon will be listed here.

Abstract

An electrochemical microbiosensor for DNA has been fabricated based on new acrylic microspheres modified with reactive N-acryloxysuccinimide (NAS) functional groups. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized in an emulsion form with a simple one-step photopolymerization technique. Aminated DNA probe was attached to the succinimde functional group of the acrylic microspheres via covalent bonding. The hybridization of the immobilized DNA probe with the complementary DNA was studied by differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. The influences of many factors such as duration of DNA probe immobilization and hybridization, pH, type of ions, buffer concentrations, ionic strength, operational temperature and non-complementary DNA on the biosensor performance were evaluated. Under optimized conditions, the DNA microbiosensor demonstrated a linear response range to target DNA over a wide concentration range of 1.0 × 10(-16) and 1.0 × 10(-8) M with a lower limit of detection (LOD) of 9.46 × 10(-17) M (R(2) = 0.97). This DNA microbiosensor showed good reproducibility with 2.84% RSD (relative standard deviation) (n = 3). Application of the NAS-modified acrylic microspheres in the construction of DNA microbiosensor had improved the overall analytical performance of the resultant DNA microbiosensor when compared with other reported DNA biosensors using other nano-materials for membranes and microspheres as DNA immobilization matrices.

Citing Articles

Development of label-free electrochemical OMP-DNA probe biosensor as a highly sensitive system to detect of citrus huanglongbing.

Kazemzadeh-Beneh H, Safarnejad M, Norouzi P, Samsampour D, Alavi S, Shaterreza D Sci Rep. 2024; 14(1):12183.

PMID: 38806617 PMC: 11133464. DOI: 10.1038/s41598-024-63112-w.

Strategies in the optimization of DNA hybridization conditions and its role in electrochemical detection of dengue virus (DENV) using response surface methodology (RSM).

Abdul Rashid J, Yusof N, Abdullah J, Shomiad Shueb R RSC Adv. 2023; 13(27):18748-18759.

PMID: 37362605 PMC: 10286818. DOI: 10.1039/d3ra00216k.

Recent Developments in Electrochemical Sensors for the Detection of Antibiotic-Resistant Bacteria.

Madhu S, Ramasamy S, Choi J Pharmaceuticals (Basel). 2022; 15(12).

PMID: 36558939 PMC: 9786047. DOI: 10.3390/ph15121488.

An electrochemical biosensor for the rapid genetic identification of Musang King durian.

Faizal Azizi M, Romeli S, Razali H, Ariffin E, Tajol Ariffin M, Heng L Sci Rep. 2022; 12(1):19324.

PMID: 36369187 PMC: 9652400. DOI: 10.1038/s41598-022-20998-8.

Utilizing Electrochemical-Based Sensing Approaches for the Detection of SARS-CoV-2 in Clinical Samples: A Review.

Zambry N, Obande G, Khalid M, Bustami Y, Hamzah H, Awang M Biosensors (Basel). 2022; 12(7).

PMID: 35884276 PMC: 9312918. DOI: 10.3390/bios12070473.

References

Li F, Chen W, Zhang S . Development of DNA electrochemical biosensor based on covalent immobilization of probe DNA by direct coupling of sol-gel and self-assembly technologies. Biosens Bioelectron. 2008; 24(4):787-92. DOI: 10.1016/j.bios.2008.06.052. View

Wang X, Yang T, Li X, Jiao K . Three-step electrodeposition synthesis of self-doped polyaniline nanofiber-supported flower-like Au microspheres for high-performance biosensing of DNA hybridization recognition. Biosens Bioelectron. 2010; 26(6):2953-9. DOI: 10.1016/j.bios.2010.11.045. View

Arora K, Chaubey A, Singhal R, Singh R, Pandey M, Samanta S . Application of electrochemically prepared polypyrrole-polyvinyl sulphonate films to DNA biosensor. Biosens Bioelectron. 2005; 21(9):1777-83. DOI: 10.1016/j.bios.2005.09.002. View

Xu S, Tu G, Peng B, Han X . Self-assembling gold nanoparticles on thiol-functionalized poly(styrene-co-acrylic acid) nanospheres for fabrication of a mediatorless biosensor. Anal Chim Acta. 2007; 570(2):151-7. DOI: 10.1016/j.aca.2006.04.020. View

Merkoci A . Nanoparticles-based strategies for DNA, protein and cell sensors. Biosens Bioelectron. 2010; 26(4):1164-77. DOI: 10.1016/j.bios.2010.07.028. View

Loaiza O, Campuzano S, Pedrero M, Pingarron J . DNA sensor based on an Escherichia coli lac Z gene probe immobilization at self-assembled monolayers-modified gold electrodes. Talanta. 2008; 73(5):838-44. DOI: 10.1016/j.talanta.2007.04.059. View

Wang R, Tombelli S, Minunni M, Spiriti M, Mascini M . Immobilisation of DNA probes for the development of SPR-based sensing. Biosens Bioelectron. 2004; 20(5):967-74. DOI: 10.1016/j.bios.2004.06.013. View

Sun W, Qin P, Gao H, Li G, Jiao K . Electrochemical DNA biosensor based on chitosan/nano-V2O5/MWCNTs composite film modified carbon ionic liquid electrode and its application to the LAMP product of Yersinia enterocolitica gene sequence. Biosens Bioelectron. 2009; 25(6):1264-70. DOI: 10.1016/j.bios.2009.10.011. View

Feng K, Yang Y, Wang Z, Jiang J, Shen G, Yu R . A nano-porous CeO(2)/Chitosan composite film as the immobilization matrix for colorectal cancer DNA sequence-selective electrochemical biosensor. Talanta. 2008; 70(3):561-5. DOI: 10.1016/j.talanta.2006.01.009. View

10.

Wong E, Gooding J . Charge transfer through DNA: A selective electrochemical DNA biosensor. Anal Chem. 2006; 78(7):2138-44. DOI: 10.1021/ac0509096. View

11.

Escosura-Muniz A, Gonzalez-Garcia M, Costa-Garcia A . DNA hybridization sensor based on aurothiomalate electroactive label on glassy carbon electrodes. Biosens Bioelectron. 2006; 22(6):1048-54. PMC: 7126624. DOI: 10.1016/j.bios.2006.04.024. View

12.

Yang H, Zhu Y . Size dependence of SiO2 particles enhanced glucose biosensor. Talanta. 2008; 68(3):569-74. DOI: 10.1016/j.talanta.2005.04.057. View

13.

Yang J, Yang T, Feng Y, Jiao K . A DNA electrochemical sensor based on nanogold-modified poly-2,6-pyridinedicarboxylic acid film and detection of PAT gene fragment. Anal Biochem. 2007; 365(1):24-30. DOI: 10.1016/j.ab.2006.12.039. View

14.

Lucarelli F, Palchetti I, Marrazza G, Mascini M . Electrochemical DNA biosensor as a screening tool for the detection of toxicants in water and wastewater samples. Talanta. 2008; 56(5):949-57. DOI: 10.1016/s0039-9140(01)00655-5. View

15.

Zammatteo N, Jeanmart L, Hamels S, Courtois S, Louette P, Hevesi L . Comparison between different strategies of covalent attachment of DNA to glass surfaces to build DNA microarrays. Anal Biochem. 2000; 280(1):143-50. DOI: 10.1006/abio.2000.4515. View

16.

Munoz-Serrano L, Guadalupe A, Vega-Bermudez E . Morphological studies of oligodeoxyribonucleotides probes covalently immobilized at polystyrene modified surfaces. J Biotechnol. 2005; 118(3):233-45. DOI: 10.1016/j.jbiotec.2005.05.008. View

17.

Zhang W, Yang T, Li X, Wang D, Jiao K . Conductive architecture of Fe2O3 microspheres/self-doped polyaniline nanofibers on carbon ionic liquid electrode for impedance sensing of DNA hybridization. Biosens Bioelectron. 2009; 25(2):428-34. DOI: 10.1016/j.bios.2009.07.032. View

18.

Uygun A . DNA hybridization electrochemical biosensor using a functionalized polythiophene. Talanta. 2009; 79(2):194-8. DOI: 10.1016/j.talanta.2009.03.049. View

19.

Chiorcea Paquim A, Diculescu V, Oretskaya T, Oliveira Brett A . AFM and electroanalytical studies of synthetic oligonucleotide hybridization. Biosens Bioelectron. 2004; 20(5):933-44. DOI: 10.1016/j.bios.2004.06.018. View

20.

Wang Y, Prokein T, Hinz M, Seliger H, Goedel W . Immobilization and hybridization of oligonucleotides on maleimido-terminated self-assembled monolayers. Anal Biochem. 2005; 344(2):216-23. DOI: 10.1016/j.ab.2005.05.041. View