Carnosine Prevents Necrotic and Apoptotic Death of Rat Thymocytes Via Ouabain-sensitive Na/K-ATPase

Overview

Journal Cell Biochem Funct

Specialties Biochemistry
Cell Biology

Date 2012 Jul 6

PMID 22763713

Citations 2

Authors

Larisa V Smolyaninova

Alexander A Dergalev

Konstantin Y Kulebyakin

David O Carpenter

Alexander A Boldyrev

Affiliations

Soon will be listed here.

Abstract

It is known that ouabain, a selective inhibitor of Na/K-ATPase, not only can cause the activation of signal cascades, which regulate the cell viability, but also can cause the accumulation of free radicals, which can evoke the oxidative stress. We have shown that the nanomolar concentrations of ouabain result in the temporary increase in the level of intracellular free radicals, but the millimolar concentration of ouabain induces a stable intracellular accumulation of free radicals in rat thymocytes. The increasing level of free radicals resulting from both low and high concentrations of ouabain can be attenuated by the antioxidant, carnosine. Moreover, the long-term incubation with ouabain leads to the cell death by necrosis and apoptosis. Ouabain-mediated apoptosis and necrosis were also abolished by carnosine.

Citing Articles

Neuroinflammation and Neutrophils: Modulation by Ouabain.

Leite J, Cavalcante-Silva L, Raissa Ribeiro M, Lima G, Scavone C, Rodrigues-Mascarenhas S Front Pharmacol. 2022; 13:824907.

PMID: 35173621 PMC: 8841582. DOI: 10.3389/fphar.2022.824907.

Much More than a Cardiotonic Steroid: Modulation of Inflammation by Ouabain.

Cavalcante-Silva L, Lima E, Carvalho D, de Sales-Neto J, Alves A, Galvao J Front Physiol. 2017; 8:895.

PMID: 29176951 PMC: 5686084. DOI: 10.3389/fphys.2017.00895.

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