The Relation of Urinary 8-OHdG, A Marker of Oxidative Stress to DNA, and Clinical Outcomes for Ischemic Stroke

Overview

Journal Open Neurol J

Publisher Bentham Open

Specialty Neurology

Date 2012 Jul 4

PMID 22754596

Citations 20

Authors

Hideto Nakajima

Ki-Ichi Unoda

Takumi Ito

Haruko Kitaoka

Fumiharu Kimura

Toshiaki Hanafusa

Affiliations

Soon will be listed here.

Abstract

Background: Oxidative stress/free radical generation after ischemic stroke contributes to neuronal cell injury. We evaluated the utility of an oxidative stress marker, urinary 8-hydroxy-2-deoxyguanosine (8-OHdG), to demonstrate an association between the changes of 8-OHdG and outcomes after acute ischemic stroke.

Methods: We enrolled 44 patients (26 males and 18 females) who visited our hospital due to acute ischemic stroke. Urine was collected on admission and on Days 7, and 8-OHdG was measured by ELISA. The relationships between 8-OHdG levels, stroke subtypes, and clinical outcomes based on the NIHSS and modified Rankin Scale (mRS) upon discharge was evaluated.

Results: In the overall cohort, the mean urinary level of 8-OHdG on Day 7 was increased than that on Day 0. The 8-OHdG levels on Day 0 were not different between patients with poor and good outcomes. However, an increasing rate from Day 0 to 7 (Δ 8-OHdG) in stroke patients with a poor outcome(mRS ≥3) was significantly higher than those with a good outcome (mRS ≤2) (2.54 vs 39.44, p = 0.004).

Conclusions: The biochemical changes related to 8-OHdG and oxidative stress may be considered a marker of ischemic brain injury and clinical outcome of ischemic stroke.

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References

Zhang W, Sato K, Hayashi T, Omori N, Nagano I, Kato S . Extension of ischemic therapeutic time window by a free radical scavenger, Edaravone, reperfused with tPA in rat brain. Neurol Res. 2004; 26(3):342-8. DOI: 10.1179/016164104225014058. View

Wu L, Chiou C, Chang P, Wu J . Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2003; 339(1-2):1-9. DOI: 10.1016/j.cccn.2003.09.010. View

Uno M, Kitazato K, Nishi K, Itabe H, Nagahiro S . Raised plasma oxidised LDL in acute cerebral infarction. J Neurol Neurosurg Psychiatry. 2003; 74(3):312-6. PMC: 1738343. DOI: 10.1136/jnnp.74.3.312. View

Saito S, Yamauchi H, Hasui Y, Kurashige J, Ochi H, Yoshida K . Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA. Res Commun Mol Pathol Pharmacol. 2001; 107(1-2):39-44. View

Martinet W, Knaapen M, De Meyer G, Herman A, Kockx M . Elevated levels of oxidative DNA damage and DNA repair enzymes in human atherosclerotic plaques. Circulation. 2002; 106(8):927-32. DOI: 10.1161/01.cir.0000026393.47805.21. View

. Special report from the National Institute of Neurological Disorders and Stroke. Classification of cerebrovascular diseases III. Stroke. 1990; 21(4):637-76. DOI: 10.1161/01.str.21.4.637. View

Chamorro A . Role of inflammation in stroke and atherothrombosis. Cerebrovasc Dis. 2004; 17 Suppl 3:1-5. DOI: 10.1159/000075297. View

Cooke M, Evans M, Herbert K, Lunec J . Urinary 8-oxo-2'-deoxyguanosine--source, significance and supplements. Free Radic Res. 2000; 32(5):381-97. DOI: 10.1080/10715760000300391. View

Taffi R, Nanetti L, Mazzanti L, Bartolini M, Vignini A, Raffaelli F . Plasma levels of nitric oxide and stroke outcome. J Neurol. 2007; 255(1):94-8. DOI: 10.1007/s00415-007-0700-y. View

10.

Madamanchi N, Vendrov A, Runge M . Oxidative stress and vascular disease. Arterioscler Thromb Vasc Biol. 2004; 25(1):29-38. DOI: 10.1161/01.ATV.0000150649.39934.13. View

11.

Kelly P, Morrow J, Ning M, Koroshetz W, Lo E, Terry E . Oxidative stress and matrix metalloproteinase-9 in acute ischemic stroke: the Biomarker Evaluation for Antioxidant Therapies in Stroke (BEAT-Stroke) study. Stroke. 2007; 39(1):100-4. DOI: 10.1161/STROKEAHA.107.488189. View

12.

Goldstein L, Samsa G . Reliability of the National Institutes of Health Stroke Scale. Extension to non-neurologists in the context of a clinical trial. Stroke. 1997; 28(2):307-10. DOI: 10.1161/01.str.28.2.307. View

13.

Doi K, Suzuki Y, Nakao A, Fujita T, Noiri E . Radical scavenger edaravone developed for clinical use ameliorates ischemia/reperfusion injury in rat kidney. Kidney Int. 2004; 65(5):1714-23. DOI: 10.1111/j.1523-1755.2004.00567.x. View

14.

El Kossi M, Zakhary M . Oxidative stress in the context of acute cerebrovascular stroke. Stroke. 2000; 31(8):1889-92. DOI: 10.1161/01.str.31.8.1889. View

15.

Musarrat J, Wani A . Prognostic and aetiological relevance of 8-hydroxyguanosine in human breast carcinogenesis. Eur J Cancer. 1996; 32A(7):1209-14. DOI: 10.1016/0959-8049(96)00031-7. View

16.

Yagi K, Kitazato K, Uno M, Tada Y, Kinouchi T, Shimada K . Edaravone, a free radical scavenger, inhibits MMP-9-related brain hemorrhage in rats treated with tissue plasminogen activator. Stroke. 2008; 40(2):626-31. DOI: 10.1161/STROKEAHA.108.520262. View

17.

Zhang N, Komine-Kobayashi M, Tanaka R, Liu M, Mizuno Y, Urabe T . Edaravone reduces early accumulation of oxidative products and sequential inflammatory responses after transient focal ischemia in mice brain. Stroke. 2005; 36(10):2220-5. DOI: 10.1161/01.STR.0000182241.07096.06. View

18.

Mizukoshi G, Katsura K, Katayama Y . Urinary 8-hydroxy-2'-deoxyguanosine and serum S100beta in acute cardioembolic stroke patients. Neurol Res. 2005; 27(6):644-6. DOI: 10.1179/016164105X25153. View

19.

Uno M, Kitazato K, Suzue A, Matsuzaki K, Harada M, Itabe H . Inhibition of brain damage by edaravone, a free radical scavenger, can be monitored by plasma biomarkers that detect oxidative and astrocyte damage in patients with acute cerebral infarction. Free Radic Biol Med. 2005; 39(8):1109-16. DOI: 10.1016/j.freeradbiomed.2005.06.001. View

20.

Cooke M, Lunec J, Evans M . Progress in the analysis of urinary oxidative DNA damage. Free Radic Biol Med. 2002; 33(12):1601-14. DOI: 10.1016/s0891-5849(02)01146-2. View