Toll-like Receptor-4 Expression in Infants with Pertussis Infection

Overview

Journal Infection

Date 2012 Jul 4

PMID 22753132

Citations 1

Authors

Z Vadasz

E Bamberger

K Ben-Tikva

M Feterman

I Srugo

A Kessel

Affiliations

Soon will be listed here.

Abstract

Purpose: Our aim was to evaluate the expression of Toll-like receptor-4 (TLR-4) and the level of interferon-gamma (IFN-γ) and interleukin-10 (IL-10) in young infants infected by Bordetella pertussis and B. parapertussis.

Methods: Twenty-two infants under the age of 3.5 months with the clinical suspicion of pertussis were enrolled in the study. Nasopharyngeal secretions were obtained for laboratory testing, and blood samples were obtained for flow cytometry and cytokine level analysis.

Results: Six infants had positive PCR results for pertussis; the other 16 infants had infections attributable to another causal agent and were used as the control group. The mean fluorescence index, used as a measure of TLR-4 expression by monocytes, was significantly lower in infants with pertussis than in the control group infants (34.32 ± 18.58 vs. 63.14 ± 28, respectively; p = 0.041). The serum IFN-γ level was also significantly lower in infants with pertussis than in the control group patients (0.41 ± 0.58 vs. 1.36 ± 1.87, respectively; p = 0.04). No differences were found in the levels of IL-10.

Conclusions: Based on these results, we suggest that TL4 expression by monocytes and serum INF-γ levels are lower in infants with positive PCR results for pertussis than in infants with a non-pertussis upper respiratory tract infection.

Citing Articles

Comparison of TLR4 Genotype and TLR4 Pathway-Related Cytokines in Different Strains of Mice in Response to Pertussis Toxin Challenge.

Wei J, Wang L, Wei C, Guang J, Wang H, Zhou J Genes (Basel). 2024; 15(11).

PMID: 39596635 PMC: 11593472. DOI: 10.3390/genes15111435.

References

Wolfe D, Karanikas A, Hester S, Kennett M, Harvill E . IL-10 induction by Bordetella parapertussis limits a protective IFN-gamma response. J Immunol. 2010; 184(3):1392-400. PMC: 5180427. DOI: 10.4049/jimmunol.0803045. View

Banus H, Vandebriel R, De Ruiter H, Dormans J, Nagelkerke N, Mooi F . Host genetics of Bordetella pertussis infection in mice: significance of Toll-like receptor 4 in genetic susceptibility and pathobiology. Infect Immun. 2006; 74(5):2596-605. PMC: 1459714. DOI: 10.1128/IAI.74.5.2596-2605.2006. View

Dirix V, Verscheure V, Goetghebuer T, Hainaut M, Debrie A, Locht C . Monocyte-derived interleukin-10 depresses the Bordetella pertussis- specific gamma interferon response in vaccinated infants. Clin Vaccine Immunol. 2009; 16(12):1816-21. PMC: 2786394. DOI: 10.1128/CVI.00314-09. View

McGuirk P, Mills K . Direct anti-inflammatory effect of a bacterial virulence factor: IL-10-dependent suppression of IL-12 production by filamentous hemagglutinin from Bordetella pertussis. Eur J Immunol. 2000; 30(2):415-22. DOI: 10.1002/1521-4141(200002)30:2<415::AID-IMMU415>3.0.CO;2-X. View

Kosters K, Riffelmann M, Wirsing von Konig C . Evaluation of a real-time PCR assay for detection of Bordetella pertussis and B. parapertussis in clinical samples. J Med Microbiol. 2001; 50(5):436-440. DOI: 10.1099/0022-1317-50-5-436. View

Banus S, Bottema R, Siezen C, Vandebriel R, Reimerink J, Mommers M . Toll-like receptor 4 polymorphism associated with the response to whole-cell pertussis vaccination in children from the KOALA study. Clin Vaccine Immunol. 2007; 14(10):1377-80. PMC: 2168111. DOI: 10.1128/CVI.00175-07. View

McGuirk P, McCann C, Mills K . Pathogen-specific T regulatory 1 cells induced in the respiratory tract by a bacterial molecule that stimulates interleukin 10 production by dendritic cells: a novel strategy for evasion of protective T helper type 1 responses by Bordetella pertussis. J Exp Med. 2002; 195(2):221-31. PMC: 2193600. DOI: 10.1084/jem.20011288. View

Lun S, Wong C, Ko F, Hui D, Lam C . Expression and functional analysis of toll-like receptors of peripheral blood cells in asthmatic patients: implication for immunopathological mechanism in asthma. J Clin Immunol. 2008; 29(3):330-42. DOI: 10.1007/s10875-008-9269-1. View

Wolfe D, Buboltz A, Harvill E . Inefficient Toll-like receptor-4 stimulation enables Bordetella parapertussis to avoid host immunity. PLoS One. 2009; 4(1):e4280. PMC: 2627931. DOI: 10.1371/journal.pone.0004280. View

10.

OGarra A, Vieira P, Vieira P, Goldfeld A . IL-10-producing and naturally occurring CD4+ Tregs: limiting collateral damage. J Clin Invest. 2004; 114(10):1372-8. PMC: 525746. DOI: 10.1172/JCI23215. View

11.

Mann P, Wolfe D, Latz E, Golenbock D, Preston A, Harvill E . Comparative toll-like receptor 4-mediated innate host defense to Bordetella infection. Infect Immun. 2005; 73(12):8144-52. PMC: 1307046. DOI: 10.1128/IAI.73.12.8144-8152.2005. View

12.

Cherry J, Grimprel E, Guiso N, Heininger U, Mertsola J . Defining pertussis epidemiology: clinical, microbiologic and serologic perspectives. Pediatr Infect Dis J. 2005; 24(5 Suppl):S25-34. DOI: 10.1097/01.inf.0000160926.89577.3b. View

13.

Ross P, Lavelle E, Mills K, Boyd A . Adenylate cyclase toxin from Bordetella pertussis synergizes with lipopolysaccharide to promote innate interleukin-10 production and enhances the induction of Th2 and regulatory T cells. Infect Immun. 2004; 72(3):1568-79. PMC: 356053. DOI: 10.1128/IAI.72.3.1568-1579.2004. View

14.

Taylor A, Verhagen J, Blaser K, Akdis M, Akdis C . Mechanisms of immune suppression by interleukin-10 and transforming growth factor-beta: the role of T regulatory cells. Immunology. 2006; 117(4):433-42. PMC: 1782242. DOI: 10.1111/j.1365-2567.2006.02321.x. View