Low Intensity Ultrasound Stimulates Osteoblast Migration at Different Frequencies

Overview

Journal J Bone Miner Metab

Specialty Endocrinology

Date 2012 Jul 4

PMID 22752127

Citations 12

Authors

Jennifer Man

Richard M Shelton

Paul R Cooper

Gabriel Landini

Ben A Scheven

Affiliations

Soon will be listed here.

Abstract

This study investigated the effects of different frequencies of low intensity ultrasound on osteoblast migration using an in vitro scratch-wound healing assay. Mouse calvarial-derived MC3T3-E1 osteoblasts in culture were exposed to continuous 45 kHz ultrasound (25 mW/cm(2)) or pulsed 1 MHz ultrasound (250 mW/cm(2)) for 30 min followed by 2 days' culture. Ultrasound treatment with either kHz or MHz output similarly and significantly increased cell numbers after 2 days in culture compared with untreated control cultures. In the scratch-wound healing assay the presence of the cell proliferation inhibitor mitomycin C (MMC) did not influence scratch-wound closure in control cultures indicating that cell migration was responsible for the in vitro wound healing. Application of ultrasound significantly stimulated wound closure. MMC did not affect kHz-stimulated in vitro wound healing; however, MMC reduced in part the scratch-wound closure rate in MHz-treated cultures suggesting that enhanced cell proliferation as well as migration was involved in the healing promoted by MHz ultrasound. In conclusion, both continuous kHz and pulsed MHz ultrasound promoted osteoblastic migration; however, subtle differences were apparent in the manner the different ultrasound regimens enhanced in vitro scratch-wound healing.

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References

Dalecki D . Mechanical bioeffects of ultrasound. Annu Rev Biomed Eng. 2004; 6:229-48. DOI: 10.1146/annurev.bioeng.6.040803.140126. View

Alter A, Rozenszajn L, MILLER H, Rosenschein U . Ultrasound inhibits the adhesion and migration of smooth muscle cells in vitro. Ultrasound Med Biol. 1998; 24(5):711-21. DOI: 10.1016/s0301-5629(98)00030-1. View

Dalla-Bona D, Tanaka E, Inubushi T, Oka H, Ohta A, Okada H . Cementoblast response to low- and high-intensity ultrasound. Arch Oral Biol. 2008; 53(4):318-23. DOI: 10.1016/j.archoralbio.2007.11.006. View

Dyson M, Brookes M . Stimulation of bone repair by ultrasound. Ultrasound Med Biol. 1983; Suppl 2:61-6. View

Korstjens C, Nolte P, Burger E, Albers G, Semeins C, Aartman I . Stimulation of bone cell differentiation by low-intensity ultrasound--a histomorphometric in vitro study. J Orthop Res. 2004; 22(3):495-500. DOI: 10.1016/j.orthres.2003.09.011. View

Pounder N, Harrison A . Low intensity pulsed ultrasound for fracture healing: a review of the clinical evidence and the associated biological mechanism of action. Ultrasonics. 2008; 48(4):330-8. DOI: 10.1016/j.ultras.2008.02.005. View

Hsu S, Huang W, Liu B, Li S, Chen H, Chang C . Effects of near-field ultrasound stimulation on new bone formation and osseointegration of dental titanium implants in vitro and in vivo. Ultrasound Med Biol. 2011; 37(3):403-16. DOI: 10.1016/j.ultrasmedbio.2010.12.004. View

Takayama T, Suzuki N, Ikeda K, Shimada T, Suzuki A, Maeno M . Low-intensity pulsed ultrasound stimulates osteogenic differentiation in ROS 17/2.8 cells. Life Sci. 2006; 80(10):965-71. DOI: 10.1016/j.lfs.2006.11.037. View

Sudo H, Kodama H, Amagai Y, Yamamoto S, Kasai S . In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 1983; 96(1):191-8. PMC: 2112252. DOI: 10.1083/jcb.96.1.191. View

10.

Man J, Shelton R, Cooper P, Scheven B . Low-intensity low-frequency ultrasound promotes proliferation and differentiation of odontoblast-like cells. J Endod. 2012; 38(5):608-13. DOI: 10.1016/j.joen.2012.01.015. View

11.

Unsworth J, Kaneez S, Harris S, Ridgway J, Fenwick S, Chenery D . Pulsed low intensity ultrasound enhances mineralisation in preosteoblast cells. Ultrasound Med Biol. 2007; 33(9):1468-74. DOI: 10.1016/j.ultrasmedbio.2006.12.003. View

12.

Scheven B, Man J, Millard J, Cooper P, Lea S, Walmsley A . VEGF and odontoblast-like cells: stimulation by low frequency ultrasound. Arch Oral Biol. 2008; 54(2):185-91. DOI: 10.1016/j.archoralbio.2008.09.008. View

13.

Katano M, Naruse K, Uchida K, Mikuni-Takagaki Y, Takaso M, Itoman M . Low intensity pulsed ultrasound accelerates delayed healing process by reducing the time required for the completion of endochondral ossification in the aged mouse femur fracture model. Exp Anim. 2011; 60(4):385-95. DOI: 10.1538/expanim.60.385. View

14.

Li J, Lin J, Liu H, Sun J, Ruaan R, Shih C . Comparison of ultrasound and electromagnetic field effects on osteoblast growth. Ultrasound Med Biol. 2006; 32(5):769-75. DOI: 10.1016/j.ultrasmedbio.2006.01.017. View

15.

Naruse K, Miyauchi A, Itoman M, Mikuni-Takagaki Y . Distinct anabolic response of osteoblast to low-intensity pulsed ultrasound. J Bone Miner Res. 2003; 18(2):360-9. DOI: 10.1359/jbmr.2003.18.2.360. View

16.

Azuma Y, Ito M, Harada Y, Takagi H, Ohta T, Jingushi S . Low-intensity pulsed ultrasound accelerates rat femoral fracture healing by acting on the various cellular reactions in the fracture callus. J Bone Miner Res. 2001; 16(4):671-80. DOI: 10.1359/jbmr.2001.16.4.671. View

17.

Rutten S, Nolte P, Korstjens C, van Duin M, Klein-Nulend J . Low-intensity pulsed ultrasound increases bone volume, osteoid thickness and mineral apposition rate in the area of fracture healing in patients with a delayed union of the osteotomized fibula. Bone. 2008; 43(2):348-354. DOI: 10.1016/j.bone.2008.04.010. View

18.

Yang R, Lin W, Chen Y, Tang C, Huang T, Lu B . Regulation by ultrasound treatment on the integrin expression and differentiation of osteoblasts. Bone. 2005; 36(2):276-83. DOI: 10.1016/j.bone.2004.10.009. View

19.

Harle J, Salih V, Mayia F, Knowles J, Olsen I . Effects of ultrasound on the growth and function of bone and periodontal ligament cells in vitro. Ultrasound Med Biol. 2001; 27(4):579-86. DOI: 10.1016/s0301-5629(00)00326-4. View

20.

Lai C, Chuang C, Li J, Chen S, Chang W . Effects of ultrasound on osteotomy healing in a rabbit fracture model. Ultrasound Med Biol. 2011; 37(10):1635-43. DOI: 10.1016/j.ultrasmedbio.2011.06.014. View