Identifying Cancer Biomarkers by Mass Spectrometry-based Glycomics

Overview

Journal Electrophoresis

Specialty Chemistry

Date 2012 Jun 29

PMID 22740464

Citations 60

Authors

Yehia Mechref

Yunli Hu

Aldo Garcia

Ahmed Hussein

Affiliations

Soon will be listed here.

Abstract

Correlations between aberrant glycosylation and cancer have been established for decades. The major advances in mass spectrometry (MS) and separation science have rapidly advanced detailed characterization of the changes associated with cancer development and progression. Over the past 10 years, many reports have described MS-based glycomic methods directed toward comparing the glycomic profiles of different human specimens collected from disease-free individuals and patients with cancers. Glycomic profiling of glycoproteins isolated from human specimens originating from disease-free individuals and patients with cancers have also been performed. Profiling of native, labeled, and permethylated glycans has been acquired using MALDI-MS and LC-MS. This review focuses on describing, discussing, and evaluating the different glycomic methods employed to characterize and quantify glycomic changes associated with cancers of different organs, including breast, colon, esophagus, liver, ovarian, pancreas, and prostate.

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