A Potential Role for B Cells in Suppressed Immune Responses in Cord Blood Transplant Recipients

Overview

Journal Bone Marrow Transplant

Specialty General Surgery

Date 2012 Jun 27

PMID 22732699

Citations 6

Authors

B C Beaudette-Zlatanova

P T Le

K L Knight

S Zhang

S Zakrzewski

M Parthasarathy

P J Stiff

Affiliations

Soon will be listed here.

Abstract

We evaluated immune reconstitution in 58 adults who received hematopoietic SCTs from allogeneic siblings (allosib), matched unrelated donors (MUD) or cord blood (CB) at 90-day intervals for 1 year post transplant. CB recipients had a higher incidence of infections in the first 100 days compared with allosib and MUD recipients. The number of circulating T cells was lower in CB recipients compared with MUD recipients at 90 days and compared with allosib recipients at 180 days. Spectratype analysis of the TCR Vβ complementarity determining region 3 (CDR3) of patient lymphocytes revealed that the TCR repertoire remained poorly diversified even at 360 days in nearly all patients. In contrast, the number of circulating B cells was significantly elevated in CB recipients compared with allosib recipients throughout the first year post transplant and compared with MUD recipients at 9-12 months. Spectratype analysis of the B-cell receptor V(H) CDR3 showed that the B-cell repertoire was diversified in most patients by 90 days. CD5(pos) B cells from assayed CB recipients expressed intracellular IL-10 early post transplant. Our data suggest that B cells, in addition to T cells, may have a role in impaired immune responses in CB transplant patients.

Citing Articles

Reconstitution of adaptive immunity after umbilical cord blood transplantation: impact on infectious complications.

Servais S, Hannon M, Peffault de Latour R, Socie G, Beguin Y Stem Cell Investig. 2017; 4:40.

PMID: 28607914 PMC: 5460101. DOI: 10.21037/sci.2017.05.03.

VH1 Family Immunoglobulin Repertoire Sequencing after Allogeneic Hematopoietic Stem Cell Transplantation.

Sethi M, Thol F, Stadler M, Heuser M, Ganser A, Koenecke C PLoS One. 2017; 12(1):e0168096.

PMID: 28095438 PMC: 5240918. DOI: 10.1371/journal.pone.0168096.

IL-10+ regulatory B cells are enriched in cord blood and may protect against cGVHD after cord blood transplantation.

Sarvaria A, Basar R, Mehta R, Shaim H, Muftuoglu M, Khoder A Blood. 2016; 128(10):1346-61.

PMID: 27439912 PMC: 5016705. DOI: 10.1182/blood-2016-01-695122.

CD5-mediated inhibition of TCR signaling proceeds normally in the absence of SHP-1.

Dong B, Somani A, Love P, Zheng X, Chen X, Zhang J Int J Mol Med. 2016; 38(1):45-56.

PMID: 27221212 PMC: 4899029. DOI: 10.3892/ijmm.2016.2592.

Risk factors for hypogammaglobulinemia after allo-SCT.

Arai Y, Yamashita K, Mizugishi K, Kondo T, Kitano T, Hishizawa M Bone Marrow Transplant. 2014; 49(6):859-61.

PMID: 24583624 DOI: 10.1038/bmt.2014.28.

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