Structure and Proposed Mechanism for the PH-sensing Helicobacter Pylori Chemoreceptor TlpB

Overview

Journal Structure

Publisher Cell Press

Specialties Biochemistry
Biotechnology
Cell Biology
Molecular Biology

Date 2012 Jun 19

PMID 22705207

Citations 54

Authors

Emily Goers Sweeney

J Nathan Henderson

John Goers

Christopher Wreden

Kevin G Hicks

Jeneva K Foster

Raghuveer Parthasarathy

S James Remington

Karen Guillemin

Affiliations

Soon will be listed here.

Abstract

pH sensing is crucial for survival of most organisms, yet the molecular basis of such sensing is poorly understood. Here, we present an atomic resolution structure of the periplasmic portion of the acid-sensing chemoreceptor, TlpB, from the gastric pathogen Helicobacter pylori. The structure reveals a universal signaling fold, a PAS domain, with a molecule of urea bound with high affinity. Through biophysical, biochemical, and in vivo mutagenesis studies, we show that urea and the urea-binding site residues play critical roles in the ability of H. pylori to sense acid. Our signaling model predicts that protonation events at Asp114, affected by changes in pH, dictate the stability of TlpB through urea binding.

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