Brassinosteroids Are Involved in Response of Cucumber (Cucumis Sativus) to Iron Deficiency

Overview

Journal Ann Bot

Publisher Oxford University Press

Specialty Biology

Date 2012 Jun 12

PMID 22684685

Citations 23

Authors

Baolan Wang

Yansu Li

Wen-Hao Zhang

Affiliations

Soon will be listed here.

Abstract

Background And Aims: Brassinosteroids (BR) are a class of plant polyhydroxysteroids with diverse functions in plant growth and development. However, there is little information on the role of BRs played in the response to nutrient deficiency.

Methods: To evaluate the role of BR in the response of plants to iron (Fe) deficiency, the effect of 24-epibrassinolide (EBR) on ferric reductase (FRO) activity, acidification of the rhizosphere and Fe content in cucumber (Cucumis sativus) seedlings under Fe-deficient (1 µm FeEDTA) and Fe-sufficient (50 µm FeEDTA) conditions were investigated.

Key Results: There was a significant increase in FRO activity upon exposure of cucumber seedlings to an Fe-deficient medium, and the Fe deficiency-induced increase in FRO activity was substantially suppressed by EBR. In contrast, application of EBR to Fe-sufficient seedlings stimulated FRO activity. Ethylene production evoked by Fe deficiency was suppressed by EBR, while EBR induced ethylene production from Fe-sufficient seedlings. Fe contents in shoots were reduced by treatment with EBR, while Fe contents in roots were markedly increased under both Fe-deficient and Fe-sufficient conditions. The reductions in Fe contents of shoots were independent of chlorophyll (CHL) contents under Fe-sufficient conditions, but they were positively correlated with CHL contents under Fe-deficient conditions. At the transcriptional level, transcripts encoding FRO (CsFRO1) and Fe transporter (CsIRT1) were increased upon exposure to the Fe-deficient medium, and the increases in transcripts were reversed by EBR.

Conclusions: The results demonstrate that BRs are likely to play a negative role in regulating Fe-deficiency-induced FRO, expressions of CsFRO1 and CsIRT1, as well as Fe translocation from roots to shoots.

Citing Articles

Brassinosteroids in Micronutrient Homeostasis: Mechanisms and Implications for Plant Nutrition and Stress Resilience.

Usmani L, Shakil A, Khan I, Alvi T, Singh S, Das D Plants (Basel). 2025; 14(4).

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A multi-omics insight on the interplay between iron deficiency and N forms in tomato.

Lodovici A, Buoso S, Miras-Moreno B, Lucini L, Tomasi N, Garcia-Perez P Front Plant Sci. 2024; 15:1408141.

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Shedding light on iron nutrition: exploring intersections of transcription factor cascades in light and iron deficiency signaling.

Trofimov K, Mankotia S, Ngigi M, Baby D, Satbhai S, Bauer P J Exp Bot. 2024; 76(3):787-802.

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The Phosphorus-Iron Nexus: Decoding the Nutrients Interaction in Soil and Plant.

Yang X, Liu C, Liang C, Wang T, Tian J Int J Mol Sci. 2024; 25(13).

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Brassinolide improves the tolerance of to alkaline stress.

Sun Z, Zou Y, Xie C, Han L, Zheng X, Tian Y Front Plant Sci. 2022; 13:1032646.

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