The Simian Varicella Virus ORF A is Expressed in Infected Cells but is Non-essential for Replication in Cell Culture

Overview

Journal Arch Virol

Specialty Microbiology

Date 2012 Jun 9

PMID 22678718

Citations 4

Authors

Wayne L Gray

Affiliations

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Abstract

The simian varicella virus (SVV) genome encodes ORF A, a truncated homolog of SVV ORF 4. The SVV ORF A was expressed as a 1.0-kb transcript in SVV-infected Vero cells. The ORF A promoter was active in infected Vero cells and was stimulated by the SVV immediate-early gene ORF 62 product (IE62), a viral transactivator of SVV genes. The SVV ORF A did not transactivate SVV IE, early, or late gene promoters in transfected Vero cells and was unable to augment IE62-mediated transactivation of SVV promoters. A SVV mutant lacking ORF A replicated as efficiently as wild-type SVV in infected Vero cells, indicating that ORF A expression is not essential for in vitro replication.

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