SOS Induction and Mutagenesis by DnaQ Missense Alleles in Wild Type Cells

Overview

Journal Mutat Res

Publisher Elsevier

Specialty Genetics

Date 2012 Jun 9

PMID 22677460

Citations 3

Authors

Satyendra Gautam

Raju Kalidindi

M Zafri Humayun

Affiliations

Soon will be listed here.

Abstract

Mistranslation leads to elevated mutagenesis and replication arrest, both of which are hypothesized to result from the presence of mixed populations of wild type and mistranslated versions of DNA polymerase III subunit proteins. Consistent with this possibility, expression of missense alleles of dnaQ (which codes for the proofreading subunit ɛ) in wild type (dnaQ+) cells is shown to lead to SOS induction as well as mutagenesis. Exposure to sublethal concentrations of streptomycin, an aminoglycoside antibiotic known to promote mistranslation, also leads to SOS induction.

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References

Maki H, Kornberg A . Proofreading by DNA polymerase III of Escherichia coli depends on cooperative interaction of the polymerase and exonuclease subunits. Proc Natl Acad Sci U S A. 1987; 84(13):4389-92. PMC: 305094. DOI: 10.1073/pnas.84.13.4389. View

Zhao J, Leung H, Winkler M . The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions. J Bacteriol. 2001; 183(5):1796-800. PMC: 95069. DOI: 10.1128/JB.183.5.1796-1800.2001. View

Drummond D, Wilke C . The evolutionary consequences of erroneous protein synthesis. Nat Rev Genet. 2009; 10(10):715-24. PMC: 2764353. DOI: 10.1038/nrg2662. View

Al Mamun A, Tominaga A, Enomoto M . Detection and characterization of the flagellar master operon in the four Shigella subgroups. J Bacteriol. 1996; 178(13):3722-6. PMC: 232628. DOI: 10.1128/jb.178.13.3722-3726.1996. View

Trewick S, Henshaw T, Hausinger R, Lindahl T, Sedgwick B . Oxidative demethylation by Escherichia coli AlkB directly reverts DNA base damage. Nature. 2002; 419(6903):174-8. DOI: 10.1038/nature00908. View

Brosius J . Superpolylinkers in cloning and expression vectors. DNA. 1989; 8(10):759-77. DOI: 10.1089/dna.1989.8.759. View

Reynolds N, Lazazzera B, Ibba M . Cellular mechanisms that control mistranslation. Nat Rev Microbiol. 2010; 8(12):849-56. DOI: 10.1038/nrmicro2472. View

Ellis N, Gallant J . An estimate of the global error frequency in translation. Mol Gen Genet. 1982; 188(2):169-72. DOI: 10.1007/BF00332670. View

Orgel L . The maintenance of the accuracy of protein synthesis and its relevance to ageing: a correction. Proc Natl Acad Sci U S A. 1970; 67(3):1476. PMC: 283377. DOI: 10.1073/pnas.67.3.1476. View

10.

Lehtinen D, Perrino F . Dysfunctional proofreading in the Escherichia coli DNA polymerase III core. Biochem J. 2004; 384(Pt 2):337-48. PMC: 1134117. DOI: 10.1042/BJ20040660. View

11.

Balashov S, Humayun M . Mistranslation induced by streptomycin provokes a RecABC/RuvABC-dependent mutator phenotype in Escherichia coli cells. J Mol Biol. 2002; 315(4):513-27. DOI: 10.1006/jmbi.2001.5273. View

12.

Dorazi R, Lingutla J, Humayun M . Expression of mutant alanine tRNAs increases spontaneous mutagenesis in Escherichia coli. Mol Microbiol. 2002; 44(1):131-41. DOI: 10.1046/j.1365-2958.2002.02847.x. View

13.

Kohanski M, Dwyer D, Hayete B, Lawrence C, Collins J . A common mechanism of cellular death induced by bactericidal antibiotics. Cell. 2007; 130(5):797-810. DOI: 10.1016/j.cell.2007.06.049. View

14.

McInerney P, Johnson A, Katz F, ODonnell M . Characterization of a triple DNA polymerase replisome. Mol Cell. 2007; 27(4):527-38. DOI: 10.1016/j.molcel.2007.06.019. View

15.

Stefan A, Reggiani L, Cianchetta S, Radeghieri A, Rodriguez A, Hochkoeppler A . Silencing of the gene coding for the epsilon subunit of DNA polymerase III slows down the growth rate of Escherichia coli populations. FEBS Lett. 2003; 546(2-3):295-9. DOI: 10.1016/s0014-5793(03)00604-5. View

16.

Gallant J, Kurland C, Parker J, Holliday R, Rosenberger R . The error catastrophe theory of aging. Point counterpoint. Exp Gerontol. 1997; 32(3):333-46. DOI: 10.1016/s0531-5565(96)00030-7. View

17.

Ren L, Al Mamun A, Humayun M . The mutA mistranslator tRNA-induced mutator phenotype requires recA and recB genes, but not the derepression of lexA-regulated functions. Mol Microbiol. 1999; 32(3):607-15. DOI: 10.1046/j.1365-2958.1999.01378.x. View

18.

Kim D, McHenry C . In vivo assembly of overproduced DNA polymerase III. Overproduction, purification, and characterization of the alpha, alpha-epsilon, and alpha-epsilon-theta subunits. J Biol Chem. 1996; 271(34):20681-9. DOI: 10.1074/jbc.271.34.20681. View

19.

Perrino F, Harvey S, McNeill S . Two functional domains of the epsilon subunit of DNA polymerase III. Biochemistry. 2000; 38(48):16001-9. DOI: 10.1021/bi991429+. View

20.

Zheng L, Baumann U, Reymond J . An efficient one-step site-directed and site-saturation mutagenesis protocol. Nucleic Acids Res. 2004; 32(14):e115. PMC: 514394. DOI: 10.1093/nar/gnh110. View