Counting Stem Cells: Methodological Constraints

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2012 Jun 7

PMID 22669654

Citations 41

Authors

Leonid V Bystrykh

Evgenia Verovskaya

Erik Zwart

Mathilde Broekhuis

Gerald de Haan

Affiliations

Soon will be listed here.

Abstract

The number of stem cells contributing to hematopoiesis has been a matter of debate. Many studies use retroviral tagging of stem cells to measure clonal contribution. Here we argue that methodological factors can impact such clonal analyses. Whereas early studies had low resolution, leading to underestimation, recent methods may result in an overestimation of stem-cell counts. We discuss how restriction enzyme choice, PCR bias, high-throughput sequencing depth and tagging method could affect the conclusions of clonal studies.

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