Arabinogalactan-protein Secretion is Associated with the Acquisition of Stigmatic Receptivity in the Apple Flower

Overview

Journal Ann Bot

Publisher Oxford University Press

Specialty Biology

Date 2012 Jun 2

PMID 22652420

Citations 20

Authors

Juan M Losada

Maria Herrero

Affiliations

Soon will be listed here.

Abstract

Background And Aims: Stigmatic receptivity plays a clear role in pollination dynamics; however, little is known about the factors that confer to a stigma the competence to be receptive for the germination of pollen grains. In this work, a developmental approach is used to evaluate the acquisition of stigmatic receptivity and its relationship with a possible change in arabinogalactan-proteins (AGPs).

Methods: Flowers of the domestic apple, Malus × domestica, were assessed for their capacity to support pollen germination at different developmental stages. Stigmas from these same stages were characterized morphologically and different AGP epitopes detected by immunocytochemistry.

Key Results: Acquisition of stigmatic receptivity and the secretion of classical AGPs from stigmatic cells occurred concurrently and following the same spatial distribution. While in unpollinated stigmas AGPs appeared unaltered, in cross-pollinated stigmas AGPs epitopes vanished as pollen tubes passed by.

Conclusions: The concurrent secretion of AGPs with the acquisition of stigmatic receptivity, together with the differential response in unpollinated and cross-pollinated pistils point out a role of AGPs in supporting pollen tube germination and strongly suggest that secretion of AGPs is associated with the acquisition of stigma receptivity.

Citing Articles

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A practical guide to in situ and ex situ characterisation of arabinogalactan proteins (AGPs) in fruits.

Kutyrieva-Nowak N, Leszczuk A, Zdunek A Plant Methods. 2023; 19(1):117.

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Functional characterization of hydroxyproline-O-galactosyltransferases for Arabidopsis arabinogalactan-protein synthesis.

Kaur D, Held M, Smith M, Showalter A BMC Plant Biol. 2021; 21(1):590.

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Effective Pollination Period and Parentage Effect on Pollen Tube Growth in Apple.

Roeder S, Serra S, Musacchi S Plants (Basel). 2021; 10(8).

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Arabinogalactan glycoprotein dynamics during the progamic phase in the tomato pistil.

Lara-Mondragon C, MacAlister C Plant Reprod. 2021; 34(2):131-148.

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