Coupling of Muscarinic Receptors to the Mobilization of Intracellular Ca2+ Stores in Permeabilized SH-SY5Y Human Neuroblastoma Cells

Overview

Journal Biochem J

Specialty Biochemistry

Date 1990 Nov 15

PMID 2264832

Citations 3

Authors

R J Wojcikiewicz

S T Safrany

R A Challiss

J Strupish

S R Nahorski

Affiliations

Soon will be listed here.

Abstract

Intracellular stores of Ca2+ were mobilized transiently by carbachol in suspensions of electrically permeabilized SH-SY5Y cells. The kinetics and the dose-dependence of this mobilization paralleled carbachol-induced increases in inositol 1,4,5-trisphosphate (InsP3) mass [for both parameters EC50 (concn. giving half-maximal response) = 60-70 microM]. Guanosine 5'-[gamma-thio]triphosphate enhanced the maximal effect and the potency of carbachol on Ca2+ mobilization and InsP3 mass, but caused separation of the dose-response curves (EC50 = 0.6 microM and 5.6 microM respectively). These data show that functional coupling of muscarinic receptors to Ca2+ mobilization can be maintained after permeabilization, reveal major effects of guanine nucleotides on agonist-induced Ca2+ mobilization and provide a basis for explanation of discrepancies between agonist potency on InsP3 concentration and Ca2+ mobilization in intact cells.

Citing Articles

Accumulation and metabolism of Ins(1,4,5)P3 and Ins(1,3,4,5)P4 in muscarinic-receptor-stimulated SH-SY5Y neuroblastoma cells.

Lambert D, Challiss R, Nahorski S Biochem J. 1991; 273 ( Pt 3):791-4.

PMID: 1996973 PMC: 1149832. DOI: 10.1042/bj2730791.

Differences in the functional responses of two cell lines each expressing Pi-hydrolysis-coupled muscarinic receptors.

BAUMGOLD J, Paek R Neurochem Res. 1992; 17(4):375-9.

PMID: 1513421 DOI: 10.1007/BF00974580.

Elevation of cytosolic calcium by cholinoceptor agonists in SH-SY5Y human neuroblastoma cells: estimation of the contribution of voltage-dependent currents.

Forsythe I, Lambert D, Nahorski S, Lindsdell P Br J Pharmacol. 1992; 107(1):207-14.

PMID: 1422573 PMC: 1907587. DOI: 10.1111/j.1476-5381.1992.tb14488.x.

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