Phosphate Import in Plants: Focus on the PHT1 Transporters

Overview

Journal Front Plant Sci

Date 2012 May 31

PMID 22645553

Citations 173

Authors

Laurent Nussaume

Satomi Kanno

Helene Javot

Elena Marin

Nathalie Pochon

Amal Ayadi

Tomoko M Nakanishi

Marie-Christine Thibaud

Affiliations

Soon will be listed here.

Abstract

The main source of phosphorus for plants is inorganic phosphate (Pi), which is characterized by its poor availability and low mobility. Uptake of this element from the soil relies heavily upon the PHT1 transporters, a specific family of plant plasma membrane proteins that were identified by homology with the yeast PHO84 Pi transporter. Since the discovery of PHT1 transporters in 1996, various studies have revealed that their function is controlled by a highly complex network of regulation. This review will summarize the current state of research on plant PHT1 multigenic families, including physiological, biochemical, molecular, cellular, and genetics studies.

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References

Liu H, Trieu A, Blaylock L, Harrison M . Cloning and characterization of two phosphate transporters from Medicago truncatula roots: regulation in response to phosphate and to colonization by arbuscular mycorrhizal (AM) fungi. Mol Plant Microbe Interact. 1998; 11(1):14-22. DOI: 10.1094/MPMI.1998.11.1.14. View

Baek S, Chung I, Yun S . Molecular cloning and characterization of a tobacco leaf cDNA encoding a phosphate transporter. Mol Cells. 2001; 11(1):1-6. View

Glassop D, Smith S, Smith F . Cereal phosphate transporters associated with the mycorrhizal pathway of phosphate uptake into roots. Planta. 2005; 222(4):688-98. DOI: 10.1007/s00425-005-0015-0. View

Franco-Zorrilla J, Martin A, Solano R, Rubio V, Leyva A, Paz-Ares J . Mutations at CRE1 impair cytokinin-induced repression of phosphate starvation responses in Arabidopsis. Plant J. 2002; 32(3):353-60. DOI: 10.1046/j.1365-313x.2002.01431.x. View

Chen J, Liu Y, Ni J, Wang Y, Bai Y, Shi J . OsPHF1 regulates the plasma membrane localization of low- and high-affinity inorganic phosphate transporters and determines inorganic phosphate uptake and translocation in rice. Plant Physiol. 2011; 157(1):269-78. PMC: 3165875. DOI: 10.1104/pp.111.181669. View

Loque D, Lalonde S, Looger L, von Wiren N, Frommer W . A cytosolic trans-activation domain essential for ammonium uptake. Nature. 2007; 446(7132):195-8. DOI: 10.1038/nature05579. View

Chiou T, Liu H, Harrison M . The spatial expression patterns of a phosphate transporter (MtPT1) from Medicago truncatula indicate a role in phosphate transport at the root/soil interface. Plant J. 2001; 25(3):281-93. DOI: 10.1046/j.1365-313x.2001.00963.x. View

Xiao K, Liu J, Dewbre G, Harrison M, Wang Z . Isolation and characterization of root-specific phosphate transporter promoters from Medicago trunatula. Plant Biol (Stuttg). 2006; 8(4):439-49. DOI: 10.1055/s-2005-873053. View

Jain A, Poling M, Karthikeyan A, Blakeslee J, Peer W, Titapiwatanakun B . Differential effects of sucrose and auxin on localized phosphate deficiency-induced modulation of different traits of root system architecture in Arabidopsis. Plant Physiol. 2007; 144(1):232-47. PMC: 1913769. DOI: 10.1104/pp.106.092130. View

10.

Misson J, Raghothama K, Jain A, Jouhet J, Block M, Bligny R . A genome-wide transcriptional analysis using Arabidopsis thaliana Affymetrix gene chips determined plant responses to phosphate deprivation. Proc Natl Acad Sci U S A. 2005; 102(33):11934-9. PMC: 1188001. DOI: 10.1073/pnas.0505266102. View

11.

Daram P, Brunner S, Persson B, Amrhein N, Bucher M . Functional analysis and cell-specific expression of a phosphate transporter from tomato. Planta. 1998; 206(2):225-33. DOI: 10.1007/s004250050394. View

12.

Hammond J, Bennett M, Bowen H, Broadley M, Eastwood D, May S . Changes in gene expression in Arabidopsis shoots during phosphate starvation and the potential for developing smart plants. Plant Physiol. 2003; 132(2):578-96. PMC: 166999. DOI: 10.1104/pp.103.020941. View

13.

Othman I, Al-Masri M . Impact of phosphate industry on the environment: a case study. Appl Radiat Isot. 2006; 65(1):131-41. DOI: 10.1016/j.apradiso.2006.06.014. View

14.

Rausch C, Bucher M . Molecular mechanisms of phosphate transport in plants. Planta. 2002; 216(1):23-37. DOI: 10.1007/s00425-002-0921-3. View

15.

Rae A, Cybinski D, Jarmey J, Smith F . Characterization of two phosphate transporters from barley; evidence for diverse function and kinetic properties among members of the Pht1 family. Plant Mol Biol. 2004; 53(1-2):27-36. DOI: 10.1023/B:PLAN.0000009259.75314.15. View

16.

Karthikeyan A, Ballachanda D, Raghothama K . Promoter deletion analysis elucidates the role of cis elements and 5'UTR intron in spatiotemporal regulation of AtPht1;4 expression in Arabidopsis. Physiol Plant. 2009; 136(1):10-8. DOI: 10.1111/j.1399-3054.2009.01207.x. View

17.

Bucher M . Functional biology of plant phosphate uptake at root and mycorrhiza interfaces. New Phytol. 2006; 173(1):11-26. DOI: 10.1111/j.1469-8137.2006.01935.x. View

18.

Karthikeyan A, Varadarajan D, Jain A, Held M, Carpita N, Raghothama K . Phosphate starvation responses are mediated by sugar signaling in Arabidopsis. Planta. 2006; 225(4):907-18. DOI: 10.1007/s00425-006-0408-8. View

19.

Kobae Y, Hata S . Dynamics of periarbuscular membranes visualized with a fluorescent phosphate transporter in arbuscular mycorrhizal roots of rice. Plant Cell Physiol. 2010; 51(3):341-53. DOI: 10.1093/pcp/pcq013. View

20.

Mukatira U, Liu C, Varadarajan D, Raghothama K . Negative regulation of phosphate starvation-induced genes. Plant Physiol. 2001; 127(4):1854-62. PMC: 133589. View