NF-kappa B: a Family of Inducible and Differentially Expressed Enhancer-binding Proteins in Human T Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Dec 1

PMID 2263603

Citations 88

Authors

J A Molitor

W H WALKER

S Doerre

D W Ballard

W C Greene

Affiliations

Soon will be listed here.

Abstract

A sensitive DNA-protein crosslinking approach has been used to characterize four inducible T-cell proteins (50 kDa, 55 kDa, 75 kDa, and 85 kDa) that specifically bind to kappa B enhancer elements. Partial proteolytic mapping revealed a distinct cleavage pattern for three of these proteins. These polypeptides are sequestered as inactive precursors in the cytosol of unstimulated T cells but can be converted into active forms in vivo by phorbol ester stimulation or in vitro by detergent treatment. The induction of these proteins by phorbol ester results in a strikingly biphasic pattern of nuclear expression with the 55-kDa and 75-kDa species appearing within minutes, whereas the 50-kDa and 85-kDa species appear only several hours after cellular stimulation. These data suggest that NF-kappa B-binding activity may not correspond to a single polypeptide but rather a family of at least four inducible and differentially regulated DNA-binding proteins that are expressed with distinct kinetics in human T lymphocytes.

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