Assembly of Aptamer Switch Probes and Photosensitizer on Gold Nanorods for Targeted Photothermal and Photodynamic Cancer Therapy

Overview

Journal ACS Nano

Publisher American Chemical Society

Specialty Biotechnology

Date 2012 May 29

PMID 22631052

Citations 80

Authors

Jian Wang

Guizhi Zhu

Mingxu You

Erqun Song

Mohammed Ibrahim Shukoor

Kejing Zhang

Meghan B Altman

Yan Chen

Zhi Zhu

Cheng Zhi Huang

Weihong Tan

Affiliations

Soon will be listed here.

Abstract

An aptamer switch probe (ASP) linking chlorin e6 (Ce6), a photosensitizer molecule, to the surface of gold nanorods (AuNRs) was used to target cancer cells for photodynamic therapy (PDT) and photothermal therapy (PTT). In the presence of target cancer cells, the ASP changes conformation to drive Ce6 away from the gold surface, thereby producing singlet oxygen for PDT upon light irradiation. Since each AuNR is modified with many ASP-Ce6 molecules, the AuNR-ASP-Ce6 conjugate yields enhanced binding and therapeutic effect by the added ability to carry many photosensitizers. In addition, absorption of radiation by the gold nanorods enables further cell destruction by the photothermal effect. Consequently, this multimodal AuNR-ASP-Ce6 conjugate offers a remarkably improved and synergistic therapeutic effect compared to PTT or PDT alone, providing high specificity and therapeutic efficiency, which can be generalized to other types of cancer therapies.

Citing Articles

A toolbox for enzymatic modification of nucleic acids with photosensitizers for photodynamic therapy.

Niogret G, Cheriaux C, Bonhomme F, Levi-Acobas F, Figliola C, Ulrich G RSC Chem Biol. 2024; 5(9):841-852.

PMID: 39211468 PMC: 11353023. DOI: 10.1039/d4cb00103f.

Mesoporous Silica-Layered Gold Nanorod Core@Silver Shell Nanostructures for Intracellular SERS Imaging and Phototherapy.

Seo S, Joe A, Han H, Manivasagan P, Jang E Pharmaceutics. 2024; 16(1).

PMID: 38276508 PMC: 10821141. DOI: 10.3390/pharmaceutics16010137.

Rationally Designed DNA-Based Scaffolds and Switching Probes for Protein Sensing.

Chamorro A, Rossetti M, Bagheri N, Porchetta A Adv Biochem Eng Biotechnol. 2024; 187:71-106.

PMID: 38273204 DOI: 10.1007/10_2023_235.

Lonidamine liposomes to enhance photodynamic and photothermal therapy of hepatocellular carcinoma by inhibiting glycolysis.

Lei L, Dai W, Man J, Hu H, Jin Q, Zhang B J Nanobiotechnology. 2023; 21(1):482.

PMID: 38102658 PMC: 10724989. DOI: 10.1186/s12951-023-02260-z.

Recent Advances in Functional Nanomaterials for Diagnostic and Sensing Using Self-Assembled Monolayers.

Basso C, Crulhas B, Castro G, Pedrosa V Int J Mol Sci. 2023; 24(13).

PMID: 37445998 PMC: 10341936. DOI: 10.3390/ijms241310819.

References

Oseroff A, Ohuoha D, Hasan T, Bommer J, Yarmush M . Antibody-targeted photolysis: selective photodestruction of human T-cell leukemia cells using monoclonal antibody-chlorin e6 conjugates. Proc Natl Acad Sci U S A. 1986; 83(22):8744-8. PMC: 387008. DOI: 10.1073/pnas.83.22.8744. View

Chari R . Targeted cancer therapy: conferring specificity to cytotoxic drugs. Acc Chem Res. 2007; 41(1):98-107. DOI: 10.1021/ar700108g. View

Shangguan D, Li Y, Tang Z, Cao Z, Chen H, Mallikaratchy P . Aptamers evolved from live cells as effective molecular probes for cancer study. Proc Natl Acad Sci U S A. 2006; 103(32):11838-43. PMC: 1567664. DOI: 10.1073/pnas.0602615103. View

Zhu Z, Tang Z, Phillips J, Yang R, Wang H, Tan W . Regulation of singlet oxygen generation using single-walled carbon nanotubes. J Am Chem Soc. 2008; 130(33):10856-7. DOI: 10.1021/ja802913f. View

Kuo W, Chang C, Chang Y, Yang M, Chien Y, Chen S . Gold nanorods in photodynamic therapy, as hyperthermia agents, and in near-infrared optical imaging. Angew Chem Int Ed Engl. 2010; 49(15):2711-5. DOI: 10.1002/anie.200906927. View

Chang M, Yang C, Huang D . Aptamer-conjugated DNA icosahedral nanoparticles as a carrier of doxorubicin for cancer therapy. ACS Nano. 2011; 5(8):6156-63. DOI: 10.1021/nn200693a. View

Wang H, Yang R, Yang L, Tan W . Nucleic acid conjugated nanomaterials for enhanced molecular recognition. ACS Nano. 2009; 3(9):2451-60. PMC: 2765789. DOI: 10.1021/nn9006303. View

Huang Y, Chang H, Tan W . Cancer cell targeting using multiple aptamers conjugated on nanorods. Anal Chem. 2008; 80(3):567-72. DOI: 10.1021/ac702322j. View

Luo Y, Shiao Y, Huang Y . Release of photoactivatable drugs from plasmonic nanoparticles for targeted cancer therapy. ACS Nano. 2011; 5(10):7796-804. DOI: 10.1021/nn201592s. View

10.

Zhen S, Chen L, Xiao S, Li Y, Hu P, Zhan L . Carbon nanotubes as a low background signal platform for a molecular aptamer beacon on the basis of long-range resonance energy transfer. Anal Chem. 2010; 82(20):8432-7. DOI: 10.1021/ac100709s. View

11.

Wang K, You M, Chen Y, Han D, Zhu Z, Huang J . Self-assembly of a bifunctional DNA carrier for drug delivery. Angew Chem Int Ed Engl. 2011; 50(27):6098-101. DOI: 10.1002/anie.201008053. View

12.

Kim D, Jeong Y, Jon S . A drug-loaded aptamer-gold nanoparticle bioconjugate for combined CT imaging and therapy of prostate cancer. ACS Nano. 2010; 4(7):3689-96. DOI: 10.1021/nn901877h. View

13.

Huang P, Li Z, Lin J, Yang D, Gao G, Xu C . Photosensitizer-conjugated magnetic nanoparticles for in vivo simultaneous magnetofluorescent imaging and targeting therapy. Biomaterials. 2011; 32(13):3447-58. DOI: 10.1016/j.biomaterials.2011.01.032. View

14.

Tang Z, Shangguan D, Wang K, Shi H, Sefah K, Mallikratchy P . Selection of aptamers for molecular recognition and characterization of cancer cells. Anal Chem. 2007; 79(13):4900-7. DOI: 10.1021/ac070189y. View

15.

Tang Z, Zhu Z, Mallikaratchy P, Yang R, Sefah K, Tan W . Aptamer-target binding triggered molecular mediation of singlet oxygen generation. Chem Asian J. 2010; 5(4):783-6. DOI: 10.1002/asia.200900545. View

16.

Barth B, Altinoglu E, Shanmugavelandy S, Kaiser J, Crespo-Gonzalez D, DiVittore N . Targeted indocyanine-green-loaded calcium phosphosilicate nanoparticles for in vivo photodynamic therapy of leukemia. ACS Nano. 2011; 5(7):5325-37. DOI: 10.1021/nn2005766. View

17.

Alkilany A, Thompson L, Boulos S, Sisco P, Murphy C . Gold nanorods: their potential for photothermal therapeutics and drug delivery, tempered by the complexity of their biological interactions. Adv Drug Deliv Rev. 2011; 64(2):190-9. DOI: 10.1016/j.addr.2011.03.005. View

18.

Tan W, Wang H, Chen Y, Zhang X, Zhu H, Yang C . Molecular aptamers for drug delivery. Trends Biotechnol. 2011; 29(12):634-40. PMC: 3218254. DOI: 10.1016/j.tibtech.2011.06.009. View

19.

Koo H, Huh M, Sun I, Yuk S, Choi K, Kim K . In vivo targeted delivery of nanoparticles for theranosis. Acc Chem Res. 2011; 44(10):1018-28. DOI: 10.1021/ar2000138. View

20.

Chin W, Heng P, Olivo M . Chlorin e6 - polyvinylpyrrolidone mediated photosensitization is effective against human non-small cell lung carcinoma compared to small cell lung carcinoma xenografts. BMC Pharmacol. 2007; 7:15. PMC: 2212622. DOI: 10.1186/1471-2210-7-15. View