The MSX1 Homeoprotein Recruits G9a Methyltransferase to Repressed Target Genes in Myoblast Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 May 26

PMID 22629437

Citations 22

Authors

Jingqiang Wang

Cory Abate-Shen

Affiliations

Soon will be listed here.

Abstract

Although the significance of lysine modifications of core histones for regulating gene expression is widely appreciated, the mechanisms by which these modifications are incorporated at specific regulatory elements during cellular differentiation remains largely unknown. In our previous studies, we have shown that in developing myoblasts the Msx1 homeoprotein represses gene expression by influencing the modification status of chromatin at its target genes. We now show that genomic binding by Msx1 promotes enrichment of the H3K9me2 mark on repressed target genes via recruitment of G9a histone methyltransferase, the enzyme responsible for catalyzing this histone mark. Interaction of Msx1 with G9a is mediated via the homeodomain and is required for transcriptional repression and regulation of cellular differentiation, as well as enrichment of the H3K9me2 mark in proximity to Msx1 binding sites on repressed target genes in myoblast cells as well as the developing limb. We propose that regulation of chromatin status by Msx1 recruitment of G9a and other histone modifying enzymes to regulatory regions of target genes represents an important means of regulating the gene expression during development.

Citing Articles

Retraction: The Msx1 Homeoprotein Recruits G9a Methyltransferase to Repressed Target Genes in Myoblast Cells.

PLoS One. 2023; 18(7):e0288411.

PMID: 37405986 PMC: 10321625. DOI: 10.1371/journal.pone.0288411.

Aberrant MNX1 expression associated with t(7;12)(q36;p13) pediatric acute myeloid leukemia induces the disease through altering histone methylation.

Waraky A, Ostlund A, Nilsson T, Weichenhan D, Lutsik P, Bahr M Haematologica. 2023; 109(3):725-739.

PMID: 37317878 PMC: 10905087. DOI: 10.3324/haematol.2022.282255.

Global Quantitative Proteomics Analysis Reveals the Downstream Signaling Networks of Msx1 and Msx2 in Myoblast Differentiation.

Zhou G, Ma S, Yang M, Yang Y Phenomics. 2023; 2(3):201-210.

PMID: 36939786 PMC: 9590559. DOI: 10.1007/s43657-022-00049-y.

Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery.

Marano N, Holaska J Front Cell Dev Biol. 2022; 10:1007120.

PMID: 36274837 PMC: 9583931. DOI: 10.3389/fcell.2022.1007120.

Histone Lysine Methylation and Long Non-Coding RNA: The New Target Players in Skeletal Muscle Cell Regeneration.

Mbadhi M, Tang J, Zhang J Front Cell Dev Biol. 2021; 9:759237.

PMID: 34926450 PMC: 8678087. DOI: 10.3389/fcell.2021.759237.

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