Diet-induced Swine Model with Obesity/leptin Resistance for the Study of Metabolic Syndrome and Type 2 Diabetes

Overview

Journal ScientificWorldJournal

Publisher Wiley

Specialty Biology

Date 2012 May 26

PMID 22629144

Citations 36

Authors

L Torres-Rovira

S Astiz

A Caro

C Lopez-Bote

C Ovilo

P Pallares

M L Perez-Solana

R Sanchez-Sanchez

A Gonzalez-Bulnes

Affiliations

Soon will be listed here.

Abstract

The objective of the present study was to determine the suitability of a swine breed with leptin resistance and predisposition to obesity (the Iberian pig) as model for studies on metabolic syndrome and type 2 diabetes. Thus, six Iberian sows had ad libitum access to food enriched with saturated fat (SFAD group; food consumption was estimated to be 4.5 kg/animal/day) whilst four females acted as controls and were fed with 2 kg/animal/day of a commercial maintenance diet. After three months of differential feeding, SFAD animals developed central obesity, dyslipidemia, insulin resistance and impaired glucose tolerance, and elevated blood pressure; the five parameters associated with the metabolic syndrome. Thus, the current study characterizes the Iberian pig as a robust, amenable, and reliable translational model for studies on nutrition-associated diseases.

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References

Koyama K, Chen G, Lee Y, Unger R . Tissue triglycerides, insulin resistance, and insulin production: implications for hyperinsulinemia of obesity. Am J Physiol. 1997; 273(4):E708-13. DOI: 10.1152/ajpendo.1997.273.4.E708. View

Liu Y, Wang Z, Yin W, Li Q, Cai M, Zhang C . Severe insulin resistance and moderate glomerulosclerosis in a minipig model induced by high-fat/ high-sucrose/ high-cholesterol diet. Exp Anim. 2007; 56(1):11-20. DOI: 10.1538/expanim.56.11. View

Mizuta E, Kokubo Y, Yamanaka I, Miyamoto Y, Okayama A, Yoshimasa Y . Leptin gene and leptin receptor gene polymorphisms are associated with sweet preference and obesity. Hypertens Res. 2008; 31(6):1069-77. DOI: 10.1291/hypres.31.1069. View

Schinner S, Scherbaum W, Bornstein S, Barthel A . Molecular mechanisms of insulin resistance. Diabet Med. 2005; 22(6):674-82. DOI: 10.1111/j.1464-5491.2005.01566.x. View

Storlien L, Jenkins A, Chisholm D, Pascoe W, Khouri S, Kraegen E . Influence of dietary fat composition on development of insulin resistance in rats. Relationship to muscle triglyceride and omega-3 fatty acids in muscle phospholipid. Diabetes. 1991; 40(2):280-9. DOI: 10.2337/diab.40.2.280. View

Arner P . Resistin: yet another adipokine tells us that men are not mice. Diabetologia. 2005; 48(11):2203-5. DOI: 10.1007/s00125-005-1956-3. View

Martin S, Qasim A, Reilly M . Leptin resistance: a possible interface of inflammation and metabolism in obesity-related cardiovascular disease. J Am Coll Cardiol. 2008; 52(15):1201-10. PMC: 4556270. DOI: 10.1016/j.jacc.2008.05.060. View

Krzesinski J, Carlier P, Rorive G . Interrelationship of hypertension, plasma lipids and atherosclerosis. Drugs. 1988; 36 Suppl 2:18-26. DOI: 10.2165/00003495-198800362-00005. View

DOUGLAS W . Of pigs and men and research: a review of applications and analogies of the pig, sus scrofa, in human medical research. Space Life Sci. 1972; 3(3):226-34. DOI: 10.1007/BF00928167. View

10.

Goldberg R . Lipid disorders in diabetes. Diabetes Care. 1981; 4(5):561-72. DOI: 10.2337/diacare.4.5.561. View

11.

Dincer U . Cardiac β-adrenoceptor expression is markedly depressed in Ossabaw swine model of cardiometabolic risk. Int J Gen Med. 2011; 4:493-9. PMC: 3133518. DOI: 10.2147/IJGM.S18175. View

12.

Roden M, Price T, Perseghin G, Petersen K, Rothman D, Cline G . Mechanism of free fatty acid-induced insulin resistance in humans. J Clin Invest. 1996; 97(12):2859-65. PMC: 507380. DOI: 10.1172/JCI118742. View

13.

Bell F, Gerrity R . Evidence for an altered lipid metabolic state in circulating blood monocytes under conditions of hyperlipemia in swine and its implications in arterial lipid metabolism. Arterioscler Thromb. 1992; 12(2):155-62. DOI: 10.1161/01.atv.12.2.155. View

14.

Dyson M, Alloosh M, Vuchetich J, Mokelke E, Sturek M . Components of metabolic syndrome and coronary artery disease in female Ossabaw swine fed excess atherogenic diet. Comp Med. 2006; 56(1):35-45. View

15.

Hall J, Brands M, Zappe D, Dixon W, Mizelle H, Reinhart G . Hemodynamic and renal responses to chronic hyperinsulinemia in obese, insulin-resistant dogs. Hypertension. 1995; 25(5):994-1002. DOI: 10.1161/01.hyp.25.5.994. View

16.

Lunney J . Advances in swine biomedical model genomics. Int J Biol Sci. 2007; 3(3):179-84. PMC: 1802015. DOI: 10.7150/ijbs.3.179. View

17.

Nesto R . Beyond low-density lipoprotein: addressing the atherogenic lipid triad in type 2 diabetes mellitus and the metabolic syndrome. Am J Cardiovasc Drugs. 2005; 5(6):379-87. DOI: 10.2165/00129784-200505060-00005. View

18.

Spurlock M, Gabler N . The development of porcine models of obesity and the metabolic syndrome. J Nutr. 2008; 138(2):397-402. DOI: 10.1093/jn/138.2.397. View

19.

Rocha E . Metabolic syndrome: an entity that lacks a clear definition but which is useful to identify in clinical practice. Rev Port Cardiol. 2009; 27(12):1531-7. View

20.

Myers M, Cowley M, Munzberg H . Mechanisms of leptin action and leptin resistance. Annu Rev Physiol. 2007; 70:537-56. DOI: 10.1146/annurev.physiol.70.113006.100707. View