Molecular Beacons in Diagnostics

Overview

Journal F1000 Med Rep

Specialty General Medicine

Date 2012 May 24

PMID 22619695

Citations 8

Authors

Sanjay Tyagi

Fred Russell Kramer

Affiliations

Soon will be listed here.

Abstract

Recent technical advances have begun to realize the potential of molecular beacons to test for diverse infections in clinical diagnostic laboratories. These include the ability to test for, and quantify, multiple pathogens in the same clinical sample, and to detect antibiotic resistant strains within hours. The design principles of molecular beacons have also spawned a variety of allied technologies.

Citing Articles

Diagnostic Assays for Avian Influenza Virus Surveillance and Monitoring in Poultry.

Azeem S, Yoon K Viruses. 2025; 17(2).

PMID: 40006983 PMC: 11860460. DOI: 10.3390/v17020228.

Fluorescent Biosensors Based on Silicon Nanowires.

Leonardi A, Lo Faro M, Fazio B, Spinella C, Conoci S, Livreri P Nanomaterials (Basel). 2021; 11(11).

PMID: 34835735 PMC: 8624671. DOI: 10.3390/nano11112970.

Biogenic Metal Nanoparticles: A New Approach to Detect Life on Mars?.

Simoes M, Ottoni C, Antunes A Life (Basel). 2020; 10(3).

PMID: 32245046 PMC: 7151574. DOI: 10.3390/life10030028.

Speciation of fungi using real time PCR with molecular beacons: Can we solve the enigma of diagnosis of invasive fungal disease?.

Kumar M, Mugunthan M, Kapoor R, Pandalanghat S Med J Armed Forces India. 2019; 75(1):41-49.

PMID: 30705477 PMC: 6349607. DOI: 10.1016/j.mjafi.2017.12.003.

Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes.

Napoli C, Lai S, Giannetti A, Tombelli S, Baldini F, Barbaro M Sensors (Basel). 2018; 18(4).

PMID: 29584638 PMC: 5948917. DOI: 10.3390/s18040990.

References

Hibbitts S, Rahman A, John R, Westmoreland D, Fox J . Development and evaluation of NucliSens basic kit NASBA for diagnosis of parainfluenza virus infection with 'end-point' and 'real-time' detection. J Virol Methods. 2003; 108(2):145-55. DOI: 10.1016/s0166-0934(02)00268-9. View

Whitcombe D, Theaker J, Guy S, Brown T, Little S . Detection of PCR products using self-probing amplicons and fluorescence. Nat Biotechnol. 1999; 17(8):804-7. DOI: 10.1038/11751. View

Chiba N, Murayama S, Morozumi M, Nakayama E, Okada T, Iwata S . Rapid detection of eight causative pathogens for the diagnosis of bacterial meningitis by real-time PCR. J Infect Chemother. 2009; 15(2):92-8. DOI: 10.1007/s10156-009-0670-3. View

El-Hajj H, Marras S, Tyagi S, Shashkina E, Kamboj M, Kiehn T . Use of sloppy molecular beacon probes for identification of mycobacterial species. J Clin Microbiol. 2009; 47(4):1190-8. PMC: 2668336. DOI: 10.1128/JCM.02043-08. View

Leone G, van Schijndel H, VAN Gemen B, Kramer F, Schoen C . Molecular beacon probes combined with amplification by NASBA enable homogeneous, real-time detection of RNA. Nucleic Acids Res. 1998; 26(9):2150-5. PMC: 147533. DOI: 10.1093/nar/26.9.2150. View

Nadal A, Coll A, Cook N, Pla M . A molecular beacon-based real time NASBA assay for detection of Listeria monocytogenes in food products: role of target mRNA secondary structure on NASBA design. J Microbiol Methods. 2007; 68(3):623-32. DOI: 10.1016/j.mimet.2006.11.011. View

Manji R, Lotlikar M, Zhang F, Ginocchio C . Clinical evaluation of NucliSENS magnetic extraction and NucliSENS analytical specific reagents for the real-time detection of respiratory syncytial virus (RSV) in paediatric respiratory specimens. J Clin Pathol. 2009; 62(11):998-1002. DOI: 10.1136/jcp.2009.066688. View

Deiman B, Schrover C, Moore C, Westmoreland D, van de Wiel P . Rapid and highly sensitive qualitative real-time assay for detection of respiratory syncytial virus A and B using NASBA and molecular beacon technology. J Virol Methods. 2007; 146(1-2):29-35. DOI: 10.1016/j.jviromet.2007.05.030. View

Vet J, Majithia A, Marras S, Tyagi S, Dube S, Poiesz B . Multiplex detection of four pathogenic retroviruses using molecular beacons. Proc Natl Acad Sci U S A. 1999; 96(11):6394-9. PMC: 26892. DOI: 10.1073/pnas.96.11.6394. View

10.

Gubala A, Proll D . Molecular-beacon multiplex real-time PCR assay for detection of Vibrio cholerae. Appl Environ Microbiol. 2006; 72(9):6424-8. PMC: 1563670. DOI: 10.1128/AEM.02597-05. View

11.

Hadjinicolaou A, Demetriou V, Emmanuel M, Kakoyiannis C, Kostrikis L . Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples. BMC Microbiol. 2009; 9:97. PMC: 2689230. DOI: 10.1186/1471-2180-9-97. View

12.

Heijnen L, Medema G . Method for rapid detection of viable Escherichia coli in water using real-time NASBA. Water Res. 2009; 43(12):3124-32. DOI: 10.1016/j.watres.2009.04.025. View

13.

Piatek A, Tyagi S, Pol A, Telenti A, MILLER L, Kramer F . Molecular beacon sequence analysis for detecting drug resistance in Mycobacterium tuberculosis. Nat Biotechnol. 1998; 16(4):359-63. DOI: 10.1038/nbt0498-359. View

14.

Lanciotti R, KERST A . Nucleic acid sequence-based amplification assays for rapid detection of West Nile and St. Louis encephalitis viruses. J Clin Microbiol. 2001; 39(12):4506-13. PMC: 88574. DOI: 10.1128/JCM.39.12.4506-4513.2001. View

15.

Boehme C, Nabeta P, Hillemann D, Nicol M, Shenai S, Krapp F . Rapid molecular detection of tuberculosis and rifampin resistance. N Engl J Med. 2010; 363(11):1005-15. PMC: 2947799. DOI: 10.1056/NEJMoa0907847. View

16.

Roy S, Kabir M, Mondal D, Ali I, Petri Jr W, Haque R . Real-time-PCR assay for diagnosis of Entamoeba histolytica infection. J Clin Microbiol. 2005; 43(5):2168-72. PMC: 1153781. DOI: 10.1128/JCM.43.5.2168-2172.2005. View

17.

Sinsimer D, Leekha S, Park S, Marras S, Koreen L, Willey B . Use of a multiplex molecular beacon platform for rapid detection of methicillin and vancomycin resistance in Staphylococcus aureus. J Clin Microbiol. 2005; 43(9):4585-91. PMC: 1234154. DOI: 10.1128/JCM.43.9.4585-4591.2005. View

18.

OShea M, Ryan M, Hawksworth A, Alsip B, Gray G . Symptomatic respiratory syncytial virus infection in previously healthy young adults living in a crowded military environment. Clin Infect Dis. 2005; 41(3):311-7. DOI: 10.1086/431591. View

19.

Stoermer R, Cederquist K, McFarland S, Sha M, Penn S, Keating C . Coupling molecular beacons to barcoded metal nanowires for multiplexed, sealed chamber DNA bioassays. J Am Chem Soc. 2006; 128(51):16892-903. PMC: 2849162. DOI: 10.1021/ja0658261. View

20.

Schneider P, Wolters L, Schoone G, Schallig H, Sillekens P, Hermsen R . Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum. J Clin Microbiol. 2005; 43(1):402-5. PMC: 540116. DOI: 10.1128/JCM.43.1.402-405.2005. View