Pph3 Dephosphorylation of Rad53 is Required for Cell Recovery from MMS-induced DNA Damage in Candida Albicans

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 May 19

PMID 22606354

Citations 11

Authors

Haitao Wang

Jiaxin Gao

Wanjie Li

Ada Hang-Heng Wong

Kangdi Hu

Kun Chen

Yue Wang

Jianli Sang

Affiliations

Soon will be listed here.

Abstract

The pathogenic fungus Candida albicans switches from yeast growth to filamentous growth in response to genotoxic stresses, in which phosphoregulation of the checkpoint kinase Rad53 plays a crucial role. Here we report that the Pph3/Psy2 phosphatase complex, known to be involved in Rad53 dephosphorylation, is required for cellular responses to the DNA-damaging agent methyl methanesulfonate (MMS) but not the DNA replication inhibitor hydroxyurea (HU) in C. albicans. Deletion of either PPH3 or PSY2 resulted in enhanced filamentous growth during MMS treatment and continuous filamentous growth even after MMS removal. Moreover, during this growth, Rad53 remained hyperphosphorylated, MBF-regulated genes were downregulated, and hypha-specific genes were upregulated. We have also identified S461 and S545 on Rad53 as potential dephosphorylation sites of Pph3/Psy2 that are specifically involved in cellular responses to MMS. Therefore, our studies have identified a novel molecular mechanism mediating DNA damage response to MMS in C. albicans.

Citing Articles

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