Isolation of an Aminoglycoside Receptor from Guinea Pig Inner Ear Tissues and Kidney

Overview

Journal Arch Otorhinolaryngol

Specialty Otorhinolaryngology

Date 1979 Jan 1

PMID 226045

Citations 26

Authors

J Schacht

Affiliations

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Abstract

Affinity chromatography was used to isolate aminoglycoside receptors from inner ear tissues and kidney. Neomycin was immobilized on glass beads and served as the stationary phase in column chromatography. Fractionation of an organic tissue extract on this matrix demonstrated two components with high affinity for neomycin: phohphatidyl inositol phosphate and phosphatidyl inositol diphosphate. The toxicity of aminoglycosides is explained on the basis of a drug-interaction with these lipids.

Citing Articles

Cisplatin and aminoglycoside antibiotics: hearing loss and its prevention.

Schacht J, Talaska A, Rybak L Anat Rec (Hoboken). 2012; 295(11):1837-50.

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Intracellular mechanisms of aminoglycoside-induced cytotoxicity.

Karasawa T, Steyger P Integr Biol (Camb). 2011; 3(9):879-86.

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New developments in aminoglycoside therapy and ototoxicity.

Xie J, Talaska A, Schacht J Hear Res. 2011; 281(1-2):28-37.

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Identification of nuclear phosphatidylinositol 4,5-bisphosphate-interacting proteins by neomycin extraction.

Lewis A, Sommer L, Arntzen M, Strahm Y, Morrice N, Divecha N Mol Cell Proteomics. 2010; 10(2):M110.003376.

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Aminoglycoside-induced phosphatidylserine externalization in sensory hair cells is regionally restricted, rapid, and reversible.

Goodyear R, Gale J, Ranatunga K, Kros C, Richardson G J Neurosci. 2008; 28(40):9939-52.

PMID: 18829952 PMC: 3326578. DOI: 10.1523/JNEUROSCI.1124-08.2008.

References

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