Alpha 2.0
Login Register
» Articles » PMID: 226030

Differentiation of Vaccine Strains and Field Isolates of Pseudorabies (Aujeszky's Disease) Virus: Thermal Sensitivity and Rabbit Virulence Markers

Overview
Journal Arch Virol
Specialty Microbiology
Date 1979 Jan 1
PMID 226030
Citations 40
Authors
K B Platt
C J MARE
P N Hinz
Affiliations
Soon will be listed here.
Abstract

Eleven cloned North American pseudorabies virus (PRV) strains and the European vaccine strains K and BUK were characterized by their thermal sensitivity and rabbit virulence markers. Heat sensitivity of the strains and isolates ranged from the highly heat resistant strain K, to the extremely heat labile strain BUK and isolate Be. The inactivation curves of each virus were transformed to the logarithmic scale and their standardized slopes and predicted virus survival values at 30 minutes were plotted against each other. The result was a distribution of points that represented a thermal sensitivity spectrum (TSS). Virus strains were subsequently categorized into 1 of 3 groups according to their position in the TSS. Viruses were also categorized into three groups according to their ability to clinically infect rabbits, their ability to produce pruritus and the time required to kill. When individual strains were described according to their marker profiles, 5 of 9 possible marker combinations were revealed. The 2 vaccine strains were each described by separate profiles. Virulent field isolates were characterized by 1 of 3 different profiles.

Citing Articles

Pseudorabies virus infection triggers pUL46-mediated phosphorylation of connexin-43 and closure of gap junctions to promote intercellular virus spread.

Tishchenko A, Romero N, Van Waesberghe C, Delva J, Vickman O, Smith G PLoS Pathog. 2025; 21(1):e1012895.

PMID: 39836710 PMC: 11774492. DOI: 10.1371/journal.ppat.1012895.

Interferon-λ Activates a Differential Response in Peripheral Neurons That Is Effective against Alpha Herpesvirus Infections.

Salazar S, Luong K, Nua T, Koyuncu O Pathogens. 2023; 12(9).

PMID: 37764950 PMC: 10536099. DOI: 10.3390/pathogens12091142.

Live Triple Gene-Deleted Pseudorabies Virus-Vectored Subunit PCV2b and CSFV Vaccine Undergoes an Abortive Replication Cycle in the TG Neurons following Latency Reactivation.

Pavulraj S, Stout R, Paulsen D, Chowdhury S Viruses. 2023; 15(2).

PMID: 36851689 PMC: 9963255. DOI: 10.3390/v15020473.

Proteomic Comparison of Three Wild-Type Pseudorabies Virus Strains and the Attenuated Bartha Strain Reveals Reduced Incorporation of Several Tegument Proteins in Bartha Virions.

Delva J, Daled S, Van Waesberghe C, Almey R, Jansens R, Deforce D J Virol. 2022; 96(24):e0115822.

PMID: 36453884 PMC: 9769387. DOI: 10.1128/jvi.01158-22.

A Triple Gene-Deleted Pseudorabies Virus-Vectored Subunit PCV2b and CSFV Vaccine Protects Pigs against PCV2b Challenge and Induces Serum Neutralizing Antibody Response against CSFV.

Pavulraj S, Pannhorst K, Stout R, Paulsen D, Carossino M, Meyer D Vaccines (Basel). 2022; 10(2).

PMID: 35214763 PMC: 8878206. DOI: 10.3390/vaccines10020305.

References
1.
Bartha A, Belak S, Benyeda J . Trypsin- and heat-resistance of some strains of the herpesvirus group. Acta Vet Acad Sci Hung. 1969; 19(1):97-9. View
2.
Smith J, Rodriguez J, McKEE A . Biological characteristics of cloned populations of herpes simplex virus types 1 and 2. Appl Microbiol. 1971; 21(2):350-7. PMC: 377175. DOI: 10.1128/am.21.2.350-357.1971. View
3.
Wallis C, Melnick J . Thermostabilization and thermosensitization of herpesvirus. J Bacteriol. 1965; 90(6):1632-7. PMC: 315871. DOI: 10.1128/jb.90.6.1632-1637.1965. View
4.
Skoda R, Brauner I, SADECKY E, Mayer V . IMMUNIZATION AGAINST AUJESZKY'S DISEASE WITH LIVE VACCINE. I. ATTENUATION OF VIRUS AND SOME PROPERTIES OF ATTENUATED STRAINS. Acta Virol. 1964; 8:1-9. View
5.
BODON L, Meszaros J, Papp-Vid G, ROMVARY J . Properties of Aujeszky's disease virus strains isolated from swine pneumonia cases. Prelininary report. Acta Vet Acad Sci Hung. 1968; 18(1):107-9. View