The MRNA Decay Pathway Regulates the Expression of the Flo11 Adhesin and Biofilm Formation in Saccharomyces Cerevisiae

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 2012 May 19

PMID 22595243

Citations 4

Authors

Tricia L Lo

Yue Qu

Nathalie Uwamahoro

Tara Quenault

Traude H Beilharz

Ana Traven

Affiliations

Soon will be listed here.

Abstract

Regulation of the FLO11 adhesin is a model for gene expression control by extracellular signals and developmental switches. We establish that the major mRNA decay pathway regulates FLO11 expression. mRNA deadenylation of transcriptional repressors of FLO11 by the exonuclease Ccr4 keeps their levels low, thereby allowing FLO11 transcription.

Citing Articles

Epitope-tagged yeast strains reveal promoter driven changes to 3'-end formation and convergent antisense-transcription from common 3' UTRs.

Swaminathan A, Beilharz T Nucleic Acids Res. 2015; 44(1):377-86.

PMID: 26481348 PMC: 4705644. DOI: 10.1093/nar/gkv1022.

Two-dimensionality of yeast colony expansion accompanied by pattern formation.

Chen L, Noorbakhsh J, Adams R, Samaniego-Evans J, Agollah G, Nevozhay D PLoS Comput Biol. 2014; 10(12):e1003979.

PMID: 25504059 PMC: 4263361. DOI: 10.1371/journal.pcbi.1003979.

Coupling mRNA synthesis and decay.

Braun K, Young E Mol Cell Biol. 2014; 34(22):4078-87.

PMID: 25154419 PMC: 4248707. DOI: 10.1128/MCB.00535-14.

The cellular roles of Ccr4-NOT in model and pathogenic fungi-implications for fungal virulence.

Panepinto J, Heinz E, Traven A Front Genet. 2014; 4:302.

PMID: 24391665 PMC: 3868889. DOI: 10.3389/fgene.2013.00302.

References

Lo W, Dranginis A . The cell surface flocculin Flo11 is required for pseudohyphae formation and invasion by Saccharomyces cerevisiae. Mol Biol Cell. 1998; 9(1):161-71. PMC: 25236. DOI: 10.1091/mbc.9.1.161. View

Hoyer L, Green C, Oh S, Zhao X . Discovering the secrets of the Candida albicans agglutinin-like sequence (ALS) gene family--a sticky pursuit. Med Mycol. 2007; 46(1):1-15. PMC: 2742883. DOI: 10.1080/13693780701435317. View

Dagley M, Gentle I, Beilharz T, Pettolino F, Djordjevic J, Lo T . Cell wall integrity is linked to mitochondria and phospholipid homeostasis in Candida albicans through the activity of the post-transcriptional regulator Ccr4-Pop2. Mol Microbiol. 2011; 79(4):968-89. DOI: 10.1111/j.1365-2958.2010.07503.x. View

Tuomi J, Voorbraak F, Jones D, Ruijter J . Bias in the Cq value observed with hydrolysis probe based quantitative PCR can be corrected with the estimated PCR efficiency value. Methods. 2010; 50(4):313-22. DOI: 10.1016/j.ymeth.2010.02.003. View

Parker R, Song H . The enzymes and control of eukaryotic mRNA turnover. Nat Struct Mol Biol. 2004; 11(2):121-7. DOI: 10.1038/nsmb724. View

Lackner D, Beilharz T, Marguerat S, Mata J, Watt S, Schubert F . A network of multiple regulatory layers shapes gene expression in fission yeast. Mol Cell. 2007; 26(1):145-55. PMC: 1885965. DOI: 10.1016/j.molcel.2007.03.002. View

Janicke A, Vancuylenberg J, Boag P, Traven A, Beilharz T . ePAT: a simple method to tag adenylated RNA to measure poly(A)-tail length and other 3' RACE applications. RNA. 2012; 18(6):1289-95. PMC: 3358650. DOI: 10.1261/rna.031898.111. View

Braus G, Grundmann O, Bruckner S, Mosch H . Amino acid starvation and Gcn4p regulate adhesive growth and FLO11 gene expression in Saccharomyces cerevisiae. Mol Biol Cell. 2003; 14(10):4272-84. PMC: 207018. DOI: 10.1091/mbc.e03-01-0042. View

Reynolds T, Fink G . Bakers' yeast, a model for fungal biofilm formation. Science. 2001; 291(5505):878-81. DOI: 10.1126/science.291.5505.878. View

10.

Park Y, Hur H, Ka M, Kim J . Identification of translational regulation target genes during filamentous growth in Saccharomyces cerevisiae: regulatory role of Caf20 and Dhh1. Eukaryot Cell. 2006; 5(12):2120-7. PMC: 1694813. DOI: 10.1128/EC.00121-06. View

11.

Goldstrohm A, Hook B, Seay D, Wickens M . PUF proteins bind Pop2p to regulate messenger RNAs. Nat Struct Mol Biol. 2006; 13(6):533-9. DOI: 10.1038/nsmb1100. View

12.

Bruckner S, Mosch H . Choosing the right lifestyle: adhesion and development in Saccharomyces cerevisiae. FEMS Microbiol Rev. 2011; 36(1):25-58. DOI: 10.1111/j.1574-6976.2011.00275.x. View

13.

Liu Y, Filler S . Candida albicans Als3, a multifunctional adhesin and invasin. Eukaryot Cell. 2010; 10(2):168-73. PMC: 3067396. DOI: 10.1128/EC.00279-10. View

14.

Kuchin S, Vyas V, Carlson M . Snf1 protein kinase and the repressors Nrg1 and Nrg2 regulate FLO11, haploid invasive growth, and diploid pseudohyphal differentiation. Mol Cell Biol. 2002; 22(12):3994-4000. PMC: 133850. DOI: 10.1128/MCB.22.12.3994-4000.2002. View

15.

Ruijter J, Ramakers C, Hoogaars W, Karlen Y, Bakker O, van den Hoff M . Amplification efficiency: linking baseline and bias in the analysis of quantitative PCR data. Nucleic Acids Res. 2009; 37(6):e45. PMC: 2665230. DOI: 10.1093/nar/gkp045. View

16.

Goldstrohm A, Wickens M . Multifunctional deadenylase complexes diversify mRNA control. Nat Rev Mol Cell Biol. 2008; 9(4):337-44. DOI: 10.1038/nrm2370. View

17.

Finkel J, Mitchell A . Genetic control of Candida albicans biofilm development. Nat Rev Microbiol. 2010; 9(2):109-18. PMC: 3891587. DOI: 10.1038/nrmicro2475. View

18.

Halme A, Bumgarner S, Styles C, Fink G . Genetic and epigenetic regulation of the FLO gene family generates cell-surface variation in yeast. Cell. 2004; 116(3):405-15. DOI: 10.1016/s0092-8674(04)00118-7. View

19.

Hook B, Goldstrohm A, Seay D, Wickens M . Two yeast PUF proteins negatively regulate a single mRNA. J Biol Chem. 2007; 282(21):15430-8. DOI: 10.1074/jbc.M611253200. View

20.

Traven A, Lo T, Lithgow T, Heierhorst J . The yeast PUF protein Puf5 has Pop2-independent roles in response to DNA replication stress. PLoS One. 2010; 5(5):e10651. PMC: 2871046. DOI: 10.1371/journal.pone.0010651. View