Single Strands Induce RecA Protein to Unwind Duplex DNA for Homologous Pairing

Overview

Journal Nature

Specialty Science

Date 1979 Sep 20

PMID 225671

Citations 46

Authors

R P Cunningham

T Shibata

C Dasgupta

C M Radding

Affiliations

Soon will be listed here.

Abstract

Single-stranded DNA, whether homologous or not, stimulates purified Escherichia coli recA protein to unwind duplex DNA. This helps to explain how recA promotes a search for homology in genetic recombination. As oligodeoxynucleotide also stimulate unwinding, a common mechanism may relate the function of recA protein in recombination to other functions (SOS) induced by oligonucleotides.

Citing Articles

Homology recognition without double-stranded DNA-strand separation in D-loop formation by RecA.

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The intrinsic ability of double-stranded DNA to carry out D-loop and R-loop formation.

Shibata T, Iwasaki W, Hirota K Comput Struct Biotechnol J. 2020; 18:3350-3360.

PMID: 33294131 PMC: 7677664. DOI: 10.1016/j.csbj.2020.10.025.

DNA Sequence Alignment during Homologous Recombination.

Greene E J Biol Chem. 2016; 291(22):11572-80.

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RecBCD is required to complete chromosomal replication: Implications for double-strand break frequencies and repair mechanisms.

Courcelle J, Wendel B, Livingstone D, Courcelle C DNA Repair (Amst). 2015; 32:86-95.

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A non-canonical DNA structure enables homologous recombination in various genetic systems.

Masuda T, Ito Y, Terada T, Shibata T, Mikawa T J Biol Chem. 2009; 284(44):30230-9.

PMID: 19729448 PMC: 2781578. DOI: 10.1074/jbc.M109.043810.