Glioma Cells Promote the Expression of Vascular Cell Adhesion Molecule-1 on Bone Marrow-derived Mesenchymal Stem Cells: a Possible Mechanism for Their Tropism Toward Gliomas

Overview

Journal J Mol Neurosci

Specialties Molecular Biology
Neurology

Date 2012 May 8

PMID 22562815

Citations 9

Authors

Yi Hu

Peng Cheng

Yi-xue Xue

Yun-Hui Liu

Affiliations

Soon will be listed here.

Abstract

The tropism of bone marrow-derived mesenchymal stem cells (BMSCs) toward gliomas has been shown by in vitro and in vivo assays. This study was carried out to evaluate the role of vascular cell adhesion molecule-1 (VCAM-1) in the migration of BMSCs towards glioma and the effect of glioma cells on the VCAM-1 expression of BMSCs. BMSCs were isolated according to their adherence to plastic. The tropism of BMSCs toward C6 and U87 glioma and the role of VCAM-1 in this migration were analyzed by in vitro migration assay, separately. Reverse transcription-polymerase chain reaction, immunofluorescence, and Western blot were employed to assess VCAM-1 expression of BMSCs when they were incubated by the conditioned mediums (CM) of C6 or U87 glioma cells. Data revealed that C6 and U87 glioma cells promote the migration of BMSCs, which could be blocked by a VCAM-1-neutralizing antibody. Moreover, VCAM-1 expression of BMSCs was elevated by the incubation of their CM. The results also demonstrated that LY294002, an inhibitor of phosphoinositide-3-kinase (PI3K), significantly inhibited the glioma-induced upregulation of VCAM-1 on BMSCs. These findings suggested that glioma-induced change in VCAM-1 expression of BMSCs may play an important role in their tropism towards glioma, and PI3K is associated with the signal transduction of this process.

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References

Yoo J, Haseley A, Bratasz A, Chiocca E, Zhang J, Powell K . Antitumor efficacy of 34.5ENVE: a transcriptionally retargeted and "Vstat120"-expressing oncolytic virus. Mol Ther. 2011; 20(2):287-97. PMC: 3277242. DOI: 10.1038/mt.2011.208. View

Sasportas L, Kasmieh R, Wakimoto H, Hingtgen S, van de Water J, Mohapatra G . Assessment of therapeutic efficacy and fate of engineered human mesenchymal stem cells for cancer therapy. Proc Natl Acad Sci U S A. 2009; 106(12):4822-7. PMC: 2660771. DOI: 10.1073/pnas.0806647106. View

Aboody K, Brown A, Rainov N, Bower K, Liu S, Yang W . Neural stem cells display extensive tropism for pathology in adult brain: evidence from intracranial gliomas. Proc Natl Acad Sci U S A. 2000; 97(23):12846-51. PMC: 18852. DOI: 10.1073/pnas.97.23.12846. View

Forte G, Minieri M, Cossa P, Antenucci D, Sala M, Gnocchi V . Hepatocyte growth factor effects on mesenchymal stem cells: proliferation, migration, and differentiation. Stem Cells. 2005; 24(1):23-33. DOI: 10.1634/stemcells.2004-0176. View

Lau C, Koty Z, Nalbantoglu J . Differential response of glioma cells to FOXO1-directed therapy. Cancer Res. 2009; 69(13):5433-40. DOI: 10.1158/0008-5472.CAN-08-4540. View

Scrima M, De Marco C, Fabiani F, Franco R, Pirozzi G, Rocco G . Signaling networks associated with AKT activation in non-small cell lung cancer (NSCLC): new insights on the role of phosphatydil-inositol-3 kinase. PLoS One. 2012; 7(2):e30427. PMC: 3281846. DOI: 10.1371/journal.pone.0030427. View

Ponte A, Marais E, Gallay N, Langonne A, Delorme B, Herault O . The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities. Stem Cells. 2007; 25(7):1737-45. DOI: 10.1634/stemcells.2007-0054. View

Nakamizo A, Marini F, Amano T, Khan A, Studeny M, Gumin J . Human bone marrow-derived mesenchymal stem cells in the treatment of gliomas. Cancer Res. 2005; 65(8):3307-18. DOI: 10.1158/0008-5472.CAN-04-1874. View

Snykers S, Vanhaecke T, Rogiers V . Isolation of rat bone marrow stem cells. Methods Mol Biol. 2006; 320:265-72. DOI: 10.1385/1-59259-998-2:265. View

10.

Okkenhaug K, Vanhaesebroeck B . PI3K in lymphocyte development, differentiation and activation. Nat Rev Immunol. 2003; 3(4):317-30. DOI: 10.1038/nri1056. View

11.

Ryu C, Park S, Park S, Kim S, Lim J, Jeong C . Gene therapy of intracranial glioma using interleukin 12-secreting human umbilical cord blood-derived mesenchymal stem cells. Hum Gene Ther. 2011; 22(6):733-43. DOI: 10.1089/hum.2010.187. View

12.

Ward S, Westwick J, Harris S . Sat-Nav for T cells: Role of PI3K isoforms and lipid phosphatases in migration of T lymphocytes. Immunol Lett. 2011; 138(1):15-8. DOI: 10.1016/j.imlet.2011.02.007. View

13.

Segers V, Van Riet I, Andries L, Lemmens K, Demolder M, De Becker A . Mesenchymal stem cell adhesion to cardiac microvascular endothelium: activators and mechanisms. Am J Physiol Heart Circ Physiol. 2005; 290(4):H1370-7. DOI: 10.1152/ajpheart.00523.2005. View

14.

Cheng P, Gao Z, Liu Y, Xue Y . Platelet-derived growth factor BB promotes the migration of bone marrow-derived mesenchymal stem cells towards C6 glioma and up-regulates the expression of intracellular adhesion molecule-1. Neurosci Lett. 2009; 451(1):52-6. DOI: 10.1016/j.neulet.2008.12.044. View

15.

Birnbaum T, Roider J, Schankin C, Padovan C, Schichor C, Goldbrunner R . Malignant gliomas actively recruit bone marrow stromal cells by secreting angiogenic cytokines. J Neurooncol. 2007; 83(3):241-7. DOI: 10.1007/s11060-007-9332-4. View

16.

Ding W, Knox T, Tschumper R, Wu W, Schwager S, Boysen J . Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch. Blood. 2010; 116(16):2984-93. PMC: 2974606. DOI: 10.1182/blood-2010-02-269894. View

17.

Ruster B, Gottig S, Ludwig R, Bistrian R, Muller S, Seifried E . Mesenchymal stem cells display coordinated rolling and adhesion behavior on endothelial cells. Blood. 2006; 108(12):3938-44. DOI: 10.1182/blood-2006-05-025098. View

18.

Jiang B, Xu S, Hou X, Pimentel D, Cohen R . Angiotensin II differentially regulates interleukin-1-beta-inducible NO synthase (iNOS) and vascular cell adhesion molecule-1 (VCAM-1) expression: role of p38 MAPK. J Biol Chem. 2004; 279(19):20363-8. DOI: 10.1074/jbc.M314172200. View

19.

Ho I, Chan K, Ng W, Guo C, Hui K, Cheang P . Matrix metalloproteinase 1 is necessary for the migration of human bone marrow-derived mesenchymal stem cells toward human glioma. Stem Cells. 2009; 27(6):1366-75. PMC: 2771102. DOI: 10.1002/stem.50. View

20.

Wang X, Feuerstein G, Gu J, Lysko P, Yue T . Interleukin-1 beta induces expression of adhesion molecules in human vascular smooth muscle cells and enhances adhesion of leukocytes to smooth muscle cells. Atherosclerosis. 1995; 115(1):89-98. DOI: 10.1016/0021-9150(94)05503-b. View