Tubulin Homolog TubZ in a Phage-encoded Partition System

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2012 Apr 28

PMID 22538818

Citations 33

Authors

Maria A Oliva

Antonio J Martin-Galiano

Yoshihiko Sakaguchi

Jose M Andreu

Affiliations

Soon will be listed here.

Abstract

Partition systems are responsible for the process whereby large and essential plasmids are accurately positioned to daughter cells during bacterial division. They are typically made of three components: a centromere-like DNA zone, an adaptor protein, and an assembling protein that is either a Walker-box ATPase (type I) or an actin-like ATPase (type II). A recently described type III segregation system has a tubulin/FtsZ-like protein, called TubZ, for plasmid movement. Here, we present the 2.3 Å structure and dynamic assembly of a TubZ tubulin homolog from a bacteriophage and unravel the Clostridium botulinum phage c-st type III partition system. Using biochemical and biophysical approaches, we prove that a gene upstream from tubZ encodes the partner TubR and localize the centromeric region (tubS), both of which are essential for anchoring phage DNA to the motile TubZ filaments. Finally, we describe a conserved fourth component, TubY, which modulates the TubZ-R-S complex interaction.

Citing Articles

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