Differential Proteome Profiling Using ITRAQ in Microalbuminuric and Normoalbuminuric Type 2 Diabetic Patients

Overview

Journal Exp Diabetes Res

Specialty Endocrinology

Date 2012 Apr 27

PMID 22536212

Citations 22

Authors

Jonghwa Jin

Yun Hyi Ku

Yikwon Kim

Yeonjung Kim

Kyunggon Kim

Ji Yoon Lee

Young Min Cho

Hong Kyu Lee

Kyong Soo Park

YoungSoo Kim

Affiliations

Soon will be listed here.

Abstract

Diabetic nephropathy (DN) is a long-term complication of diabetes mellitus that leads to end-stage renal disease. Microalbuminuria is used for the early detection of diabetic renal damage, but such levels do not reflect the state of incipient DN precisely in type 2 diabetic patients because microalbuminuria develops in other diseases, necessitating more accurate biomarkers that detect incipient DN. Isobaric tags for relative and absolute quantification (iTRAQ) were used to identify urinary proteins that were differentially excreted in normoalbuminuric and microalbuminuric patients with type 2 diabetes where 710 and 196 proteins were identified and quantified, respectively. Some candidates were confirmed by 2-DE analysis, or validated by Western blot and multiple reaction monitoring (MRM). Specifically, some differentially expressed proteins were verified by MRM in urine from normoalbuminuric and microalbuminuric patients with type 2 diabetes, wherein alpha-1-antitrypsin, alpha-1-acid glycoprotein 1, and prostate stem cell antigen had excellent AUC values (0.849, 0.873, and 0.825, resp.). Moreover, we performed a multiplex assay using these biomarker candidates, resulting in a merged AUC value of 0.921. Although the differentially expressed proteins in this iTRAQ study require further validation in larger and categorized sample groups, they constitute baseline data on preliminary biomarker candidates that can be used to discover novel biomarkers for incipient DN.

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