Screening Togolese Medicinal Plants for Few Pharmacological Properties

Overview

Journal Pharmacognosy Res

Date 2012 Apr 21

PMID 22518084

Citations 2

Authors

Simplice D Karou

Tchadjobo Tchacondo

Micheline Agassounon Djikpo Tchibozo

Kokou Anani

Lassina Ouattara

Jacques Simpore

Comlan De Souza

Affiliations

Soon will be listed here.

Abstract

Background: Terminalia macroptera Guill. et Perr. (Combretaceae), Sida alba L. (Malvaceae), Prosopis africana Guill et Perr. Taub. (Mimosaceae), Bridelia ferruginea Benth. (Euphorbiaceae), and Vetiveria nigritana Stapf. (Asteraceae) are traditionally used in Togolese folk medicine to treat several diseases including microbial infections.

Objective: This study aimed to investigate the antimicrobial, antioxidant, and hemolytic properties of the crude extracts of the above-mentioned plants.

Materials And Methods: The antimicrobial and the antioxidant activities were assayed using the NCCLS microdilution method and the DPPH free radical scavenging, respectively. Human A+ red blood cells were used to perform the hemolytic assay. Phenolics were further quantified in the extracts using spectrophotometric methods.

Results: Minimal inhibitory concentrations in the range of 230-1800 μg/ml were recorded in the NCCLS broth microdilution for both bacterial and fungal strains with methanol extracts. The DPPH radical scavenging assay yielded interesting antioxidant activities of the extracts of P. africana and T. macroptera (IC(50) values of 0.003 ± 0.00 μg/ml and 0.05 ± 0.03 μg/ml, respectively). These activities were positively correlated with the total phenolic contents and negatively correlated with the proanthocyanidin content of the extracts. The hemolytic assay revealed that great hemolysis occurred with the methanol extracts of T. macroptera, S. longepedunculata, and B. ferruginea.

Conclusion: These results support in part the use of the selected plants in the treatment of microbial infections. In addition, the plant showed an interesting antioxidant activity that could be useful in the management of oxidative stress.

Citing Articles

Ethnopharmacology, Chemistry and Biological Properties of Four Malian Medicinal Plants.

Malterud K Plants (Basel). 2017; 6(1).

PMID: 28230801 PMC: 5371770. DOI: 10.3390/plants6010011.

Ethnobotanical Study of Latex Plants in the Maritime Region of Togo.

Hoekou Y, Tchacondo T, Karou S, Koudouvo K, Atakpama W, Pissang P Pharmacognosy Res. 2016; 8(2):128-34.

PMID: 27034604 PMC: 4780139. DOI: 10.4103/0974-8490.175613.

References

Conrad J, Vogler B, Klaiber I, Reeb S, Guse J, Roos G . Vanillic acid 4-O-beta-D-(6'-O-galloyl) glucopyranoside and other constituents from the bark of Terminalia macroptera Guill.et Perr. Nat Prod Lett. 2001; 15(1):35-42. DOI: 10.1080/10575630108041255. View

Mariita R, Orodho J, Okemo P, Mbugua P . Antifungal, antibacterial and antimycobacterial activity of Entada abysinnica Steudel ex A. Rich (Fabaceae) methanol extract. Pharmacognosy Res. 2011; 2(3):163-8. PMC: 3141308. DOI: 10.4103/0974-8490.65511. View

Akomo E, Zongo C, Karou S, Obame L, Savadogo A, Atteke C . In vitro antiplasmodial and antibacterial activities of Canthium multiflorum Schum and Thonn (Rubiacea) extracts. Pak J Biol Sci. 2009; 12(12):919-23. DOI: 10.3923/pjbs.2009.919.923. View

Singh M, Khatoon S, Singh S, Kumar V, Rawat A, Mehrotra S . Antimicrobial screening of ethnobotanically important stem bark of medicinal plants. Pharmacognosy Res. 2011; 2(4):254-7. PMC: 3141137. DOI: 10.4103/0974-8490.69127. View

Tchacondo T, Karou S, Batawila K, Agban A, Ouro-Bangna K, Anani K . Herbal remedies and their adverse effects in Tem tribe traditional medicine in Togo. Afr J Tradit Complement Altern Med. 2012; 8(1):45-60. PMC: 3218446. DOI: 10.4314/ajtcam.v8i1.60522. View

Baumann E, Stoya G, Volkner A, Richter W, Lemke C, Linss W . Hemolysis of human erythrocytes with saponin affects the membrane structure. Acta Histochem. 2000; 102(1):21-35. DOI: 10.1078/0065-1281-00534. View

Karioti A, Hadjipavlou-Litina D, Mensah M, Fleischer T, Skaltsa H . Composition and antioxidant activity of the essential oils of Xylopia aethiopica (Dun) A. Rich. (Annonaceae) leaves, stem bark, root bark, and fresh and dried fruits, growing in Ghana. J Agric Food Chem. 2004; 52(26):8094-8. DOI: 10.1021/jf040150j. View

Hollinworth H . The management of infected wounds. Prof Nurse. 1997; 12(12 Suppl):S8-11. View

Conrad J, Vogler B, Reeb S, Klaiber I, Papajewski S, Roos G . Isoterchebulin and 4,6-O-isoterchebuloyl-D-glucose, novel hydrolyzable tannins from Terminalia macroptera. J Nat Prod. 2001; 64(3):294-9. DOI: 10.1021/np000506v. View

10.

Adamu H, Abayeh O, Agho M, Abdullahi A, Uba A, Dukku H . An ethnobotanical survey of Bauchi State herbal plants and their antimicrobial activity. J Ethnopharmacol. 2005; 99(1):1-4. DOI: 10.1016/j.jep.2004.12.025. View

11.

Silva O, Duarte A, Pimentel M, Viegas S, Barroso H, Machado J . Antimicrobial activity of Terminalia macroptera root. J Ethnopharmacol. 1997; 57(3):203-7. DOI: 10.1016/s0378-8741(97)00068-8. View

12.

Upadhyay B, Parveen , Dhaker A, Kumar A . Ethnomedicinal and ethnopharmaco-statistical studies of Eastern Rajasthan, India. J Ethnopharmacol. 2010; 129(1):64-86. DOI: 10.1016/j.jep.2010.02.026. View

13.

Adetutu A, Morgan W, Corcoran O . Ethnopharmacological survey and in vitro evaluation of wound-healing plants used in South-western Nigeria. J Ethnopharmacol. 2011; 137(1):50-6. DOI: 10.1016/j.jep.2011.03.073. View

14.

Nadembega P, Boussim J, Nikiema J, Poli F, Antognoni F . Medicinal plants in Baskoure, Kourittenga Province, Burkina Faso: an ethnobotanical study. J Ethnopharmacol. 2010; 133(2):378-95. DOI: 10.1016/j.jep.2010.10.010. View

15.

Sharma P, Sharma J . In vitro hemolysis of human erythrocytes -- by plant extracts with antiplasmodial activity. J Ethnopharmacol. 2001; 74(3):239-43. DOI: 10.1016/s0378-8741(00)00370-6. View

16.

Grinberg L, Newmark H, Kitrossky N, Rahamim E, Chevion M, Rachmilewitz E . Protective effects of tea polyphenols against oxidative damage to red blood cells. Biochem Pharmacol. 1997; 54(9):973-8. DOI: 10.1016/s0006-2952(97)00155-x. View

17.

Zhang A, Zhu Q, Luk Y, Ho K, Fung K, Chen Z . Inhibitory effects of jasmine green tea epicatechin isomers on free radical-induced lysis of red blood cells. Life Sci. 1997; 61(4):383-94. DOI: 10.1016/s0024-3205(97)00395-0. View

18.

Dini I, Schettino O, Simioli T, Dini A . Studies on the constituents of Chenopodium quinoa seeds: isolation and characterization of new triterpene saponins. J Agric Food Chem. 2001; 49(2):741-6. DOI: 10.1021/jf000971y. View

19.

Adetutu A, Morgan W, Corcoran O . Antibacterial, antioxidant and fibroblast growth stimulation activity of crude extracts of Bridelia ferruginea leaf, a wound-healing plant of Nigeria. J Ethnopharmacol. 2010; 133(1):116-9. DOI: 10.1016/j.jep.2010.09.011. View

20.

Sparg S, Light M, Van Staden J . Biological activities and distribution of plant saponins. J Ethnopharmacol. 2004; 94(2-3):219-43. DOI: 10.1016/j.jep.2004.05.016. View