Exchange of Free Cholesterol Between Plasma and Erythrocytes from Hyperthyroid and Hypothyroid Rats in Vitro

Overview

Journal Lipids

Specialty Biochemistry

Date 1990 Sep 1

PMID 2250589

Citations 1

Authors

F M Ruggiero

F Cafagna

E Quagliariello

Affiliations

Soon will be listed here.

Abstract

In our previous studies, we found that circulating thyroid hormone levels alter cholesterol partition between plasma and erythrocytes by changing the phospholipid content of erythrocytes (Ruggiero, F. M., et al. (1984) Horm. Metabol. Res. 16, 37-40; Ruggiero, F. M., et al. (1987) Lipids 22, 148-151). As an extension of this work, we now followed the exchange of free cholesterol between plasma and erythrocytes in control, hyperthyroid and hypothyroid rats under various experimental conditions in vitro. In control rats, erythrocytes incubated with plasma at 37 degrees C for 4 hr lose 10% of cholesterol which was esterified by lecithin:cholesterol acyltransferase (LCAT) present in the plasma. In hyperthyroid rats, erythrocytes incubated with plasma lose 30% of cholesterol within the same time. By contrast, in the case of hypothyroid rats incubation for 4 hr was necessary to transfer 24% of free cholesterol from plasma to erythrocytes. Inhibition of cholesterol esterification did not affect the loss of erythrocyte cholesterol in control and in hyperthyroid rats. Ca2+ increased the LCAT activity in the plasma of these rats. The findings shed light on the role of thyroid hormones in regulating cholesterol levels in plasma through active cholesterol transfer between plasma and erythrocytes.

Citing Articles

Alterations in osmotic fragility of the red blood cells in hypo- and hyperthyroid patients.

Zahedi Asl S, Brojeni N, Ghasemi A, Faraji F, Hedayati M, Azizi F J Endocrinol Invest. 2009; 32(1):28-32.

PMID: 19337011 DOI: 10.1007/BF03345674.

References

Ruggiero F, Gnoni G, Quagliariello E . Lipid composition of brown adipose tissue mitochondria and microsomes in hyperthyroid rats. Int J Biochem. 1989; 21(3):327-32. DOI: 10.1016/0020-711x(89)90191-2. View

Allan D, Michell R . A calcium-activated polyphosphoinositide phosphodiesterase in the plasma membrane of human and rabbit erythrocytes. Biochim Biophys Acta. 1978; 508(2):277-86. DOI: 10.1016/0005-2736(78)90330-9. View

Gnoni G, LANDRISCINA C, Quagliariello E . Thyroid hormone stimulation of Lipogenesis in isolated rat hepatocytes. Biochem Med. 1980; 24(3):336-47. DOI: 10.1016/0006-2944(80)90028-9. View

Mathe D, Chevallier F . Effects of the thyroid state on cholesterol metabolism in the rat. Biochim Biophys Acta. 1976; 441(1):155-64. DOI: 10.1016/0005-2760(76)90290-3. View

Magot T, Frein Y, Giraud F, Cheruy A . The measurement in vivo of the rate of unesterified cholesterol exchange between rat plasma and erythrocytes. Biochim Biophys Acta. 1985; 834(3):331-5. DOI: 10.1016/0005-2760(85)90006-2. View

Lange Y, CUTLER H, Steck T . The effect of cholesterol and other intercalated amphipaths on the contour and stability of the isolated red cell membrane. J Biol Chem. 1980; 255(19):9331-7. View

Ruggiero F, LANDRISCINA C, Gnoni G, Quagliariello E . Alteration of plasma and erythrocyte membrane lipid components in hyperthyroid rats. Horm Metab Res. 1984; 16(1):37-40. DOI: 10.1055/s-2007-1014688. View

Ruggiero F, LANDRISCINA C, Gnoni G, Quagliariello E . Lipid composition of liver mitochondria and microsomes in hyperthyroid rats. Lipids. 1984; 19(3):171-8. DOI: 10.1007/BF02534794. View

Brasaemle D, Robertson A, Attie A . Transbilayer movement of cholesterol in the human erythrocyte membrane. J Lipid Res. 1988; 29(4):481-9. View

10.

Ruggiero F, Gnoni G, Quagliariello E . Effect of hypothyroidism on the lipid composition of rat plasma and erythrocyte membranes. Lipids. 1987; 22(3):148-51. DOI: 10.1007/BF02537293. View

11.

Hollander d F, Chevallier F . Movement of cholesterol in vitro in rat blood and quantitation of the exchange of free cholesterol between plasma and erythrocytes. J Lipid Res. 1972; 13(6):733-44. View

12.

Bell F . Lipid exchange and transfer between biological lipid-protein structures. Prog Lipid Res. 1978; 17(2):207-43. DOI: 10.1016/0079-6832(78)90008-3. View

13.

Paradies G, Ruggiero F . Effect of hyperthyroidism on the transport of pyruvate in rat-heart mitochondria. Biochim Biophys Acta. 1988; 935(1):79-86. DOI: 10.1016/0005-2728(88)90110-7. View

14.

Dory L, Roheim P . Rat plasma lipoproteins and apolipoproteins in experimental hypothyroidism. J Lipid Res. 1981; 22(2):287-96. View

15.

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

16.

DUNCAN I, Culbreth P, Burtis C . Determination of free, total, and esterified cholesterol by high-performance liquid chromatography. J Chromatogr. 1979; 162(3):281-92. DOI: 10.1016/s0378-4347(00)81515-7. View

17.

Giraud F, Mzali H, Chailley B, Mazet F . Changes in morphology and in polyphosphoinositide turnover of human erythrocytes after cholesterol depletion. Biochim Biophys Acta. 1984; 778(1):191-200. DOI: 10.1016/0005-2736(84)90462-0. View

18.

Lange Y, Dolde J, Steck T . The rate of transmembrane movement of cholesterol in the human erythrocyte. J Biol Chem. 1981; 256(11):5321-3. View

19.

Bjornson L, Gniewkowski C, KAYDEN H . Comparison of exchange of alpha-tocopherol and free cholesterol between rat plasma lipoproteins and erythrocytes. J Lipid Res. 1975; 16(1):39-53. View

20.

Borochov H, ZAHLER P, Wilbrandt W, Shinitzky M . The effect of phosphatidylcholine to sphingomyelin mole ratio on the dynamic properties of sheep erythrocyte membrane. Biochim Biophys Acta. 1977; 470(3):382-8. DOI: 10.1016/0005-2736(77)90129-8. View