Picosecond Kinetics of Light Harvesting and Photoprotective Quenching in Wild-type and Mutant Phycobilisomes Isolated from the Cyanobacterium Synechocystis PCC 6803

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2012 Apr 17

PMID 22500770

Citations 40

Authors

Lijin Tian

Michal Gwizdala

Ivo H M van Stokkum

Rob B M Koehorst

Diana Kirilovsky

Herbert van Amerongen

Affiliations

Soon will be listed here.

Abstract

In high light conditions, cyanobacteria dissipate excess absorbed energy as heat in the light-harvesting phycobilisomes (PBs) to protect the photosynthetic system against photodamage. This process requires the binding of the red active form of the Orange Carotenoid Protein (OCP(r)), which can effectively quench the excited state of one of the allophycocyanin bilins. Recently, an in vitro reconstitution system was developed using isolated OCP and isolated PBs from Synechocystis PCC 6803. Here we have used spectrally resolved picosecond fluorescence to study wild-type and two mutated PBs. The results demonstrate that the quenching for all types of PBs takes place on an allophycocyanin bilin emitting at 660 nm (APC(Q)(660)) with a molecular quenching rate that is faster than (1 ps)(-1). Moreover, it is concluded that both the mechanism and the site of quenching are the same in vitro and in vivo. Thus, utilization of the in vitro system should make it possible in the future to elucidate whether the quenching is caused by charge transfer between APC(Q)(660) and OCP or by excitation energy transfer from APC(Q)(660) to the S(1) state of the carotenoid--a distinction that is very hard, if not impossible, to make in vivo.

Citing Articles

The Orange Carotenoid Protein Triggers Cyanobacterial Photoprotection by Quenching Bilins via a Structural Switch of Its Carotenoid.

Liguori N, van Stokkum I, Muzzopappa F, Kennis J, Kirilovsky D, Croce R J Am Chem Soc. 2024; 146(31):21913-21921.

PMID: 39058977 PMC: 11311238. DOI: 10.1021/jacs.4c06695.

Structure and function of the light-protective orange carotenoid protein families.

Garcia-Oneto T, Moyano-Bellido C, Dominguez-Martin M Curr Res Struct Biol. 2024; 7:100141.

PMID: 38736459 PMC: 11087925. DOI: 10.1016/j.crstbi.2024.100141.

Intramolecular charge transfer and the function of vibronic excitons in photosynthetic light harvesting.

Beck W Photosynth Res. 2024; 162(2-3):139-156.

PMID: 38656684 DOI: 10.1007/s11120-024-01095-5.

Structural and quantum chemical basis for OCP-mediated quenching of phycobilisomes.

Sauer P, Cupellini L, Sutter M, Bondanza M, Dominguez Martin M, Kirst H Sci Adv. 2024; 10(14):eadk7535.

PMID: 38578996 PMC: 10997198. DOI: 10.1126/sciadv.adk7535.

Energy transfer from phycobilisomes to photosystem I at 77 K.

van Stokkum I, Akhtar P, Biswas A, Lambrev P Front Plant Sci. 2023; 14:1293813.

PMID: 38078099 PMC: 10702739. DOI: 10.3389/fpls.2023.1293813.

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