Quantitative Proteomic Study of Human Lung Squamous Carcinoma and Normal Bronchial Epithelial Acquired by Laser Capture Microdissection

Overview

Journal J Biomed Biotechnol

Specialty Biology

Date 2012 Apr 14

PMID 22500095

Citations 11

Authors

Yan Xu

Lan-Qin Cao

Long-Yu Jin

Zhu-Chu Chen

Gu-Qing Zeng

Can-E Tang

Guo-Qing Li

Chao-Jun Duan

Fang Peng

Zhi-Qiang Xiao

Cui Li

Affiliations

Soon will be listed here.

Abstract

Objective: To investigate the differential protein profile of human lung squamous carcinoma (HLSC) and normal bronchial epithelium (NBE) and provide preliminary results for further study to explore the carcinogenic mechanism of HLSC.

Methods: Laser capture microdissection (LCM) was used to purify the target cells from 10 pairs of HLSC tissues and their matched NHBE, respectively. A stable-isotope labeled strategy using iTRAQ, followed by 2D-LC/Q-STAR mass spectrometry, was performed to separate and identify the differential expression proteins.

Results: A total of 96 differential expression proteins in the LCM-purified HLSC and NBE were identified. Compared with NBE, 49 proteins were upregulated and 47 proteins were downregulated in HLSC. Furthermore, the expression levels of the differential proteins including HSPB1, CKB, SCCA1, S100A8, as well as S100A9 were confirmed by western blot and tissue microarray and were consistent with the results of quantitative proteomics.

Conclusion: The different expression proteins in HLSC will provide scientific foundation for further study to explore the carcinogenic mechanism of HLSC.

Citing Articles

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References

Suminami Y, Nagashima S, Murakami A, Nawata S, Gondo T, Hirakawa H . Suppression of a squamous cell carcinoma (SCC)-related serpin, SCC antigen, inhibits tumor growth with increased intratumor infiltration of natural killer cells. Cancer Res. 2001; 61(5):1776-80. View

Ruginis T, Taglia L, Matusiak D, Lee B, Benya R . Consequence of gastrin-releasing peptide receptor activation in a human colon cancer cell line: a proteomic approach. J Proteome Res. 2006; 5(6):1460-8. DOI: 10.1021/pr060005g. View

Siu K, Desouza L, Scorilas A, Romaschin A, Honey R, Stewart R . Differential protein expressions in renal cell carcinoma: new biomarker discovery by mass spectrometry. J Proteome Res. 2009; 8(8):3797-807. DOI: 10.1021/pr800389e. View

Ichikawa M, Williams R, Wang L, Vogl T, Srikrishna G . S100A8/A9 activate key genes and pathways in colon tumor progression. Mol Cancer Res. 2011; 9(2):133-48. PMC: 3078037. DOI: 10.1158/1541-7786.MCR-10-0394. View

Hashimoto K, Kiyoshima T, Matsuo K, Ozeki S, Sakai H . Effect of SCCA1 and SCCA2 on the suppression of TNF-alpha-induced cell death by impeding the release of mitochondrial cytochrome c in an oral squamous cell carcinoma cell line. Tumour Biol. 2005; 26(4):165-72. DOI: 10.1159/000086949. View

Kim S, Cheon K, Kim C, Yoon J, Hawke D, Kobayashi R . Proteomics-based identification of proteins secreted in apical surface fluid of squamous metaplastic human tracheobronchial epithelial cells cultured by three-dimensional organotypic air-liquid interface method. Cancer Res. 2007; 67(14):6565-73. PMC: 2958044. DOI: 10.1158/0008-5472.CAN-06-2783. View

Bonner R, Cole K, Pohida T, Chuaqui R, Goldstein S, Liotta L . Laser capture microdissection: molecular analysis of tissue. Science. 1997; 278(5342):1481,1483. DOI: 10.1126/science.278.5342.1481. View

Quarta S, Vidalino L, Turato C, Ruvoletto M, Calabrese F, Valente M . SERPINB3 induces epithelial-mesenchymal transition. J Pathol. 2010; 221(3):343-56. DOI: 10.1002/path.2708. View

Neubauer H, Clare S, Kurek R, Fehm T, Wallwiener D, Sotlar K . Breast cancer proteomics by laser capture microdissection, sample pooling, 54-cm IPG IEF, and differential iodine radioisotope detection. Electrophoresis. 2006; 27(9):1840-52. DOI: 10.1002/elps.200500739. View

10.

Arai K, Teratani T, Nozawa R, Yamada T . Immunohistochemical investigation of S100A9 expression in pulmonary adenocarcinoma: S100A9 expression is associated with tumor differentiation. Oncol Rep. 2001; 8(3):591-6. DOI: 10.3892/or.8.3.591. View

11.

Guo K, Kang N, Li Y, Sun L, Gan L, Cui F . Regulation of HSP27 on NF-kappaB pathway activation may be involved in metastatic hepatocellular carcinoma cells apoptosis. BMC Cancer. 2009; 9:100. PMC: 2681475. DOI: 10.1186/1471-2407-9-100. View

12.

Garrido C, Fromentin A, Bonnotte B, Favre N, Moutet M, Arrigo A . Heat shock protein 27 enhances the tumorigenicity of immunogenic rat colon carcinoma cell clones. Cancer Res. 1998; 58(23):5495-9. View

13.

Guo H, Bai Y, Xu P, Hu Z, Liu L, Wang F . Functional promoter -1271G>C variant of HSPB1 predicts lung cancer risk and survival. J Clin Oncol. 2010; 28(11):1928-35. DOI: 10.1200/JCO.2009.24.4954. View

14.

Shekouh A, Thompson C, Prime W, Campbell F, Hamlett J, Herrington C . Application of laser capture microdissection combined with two-dimensional electrophoresis for the discovery of differentially regulated proteins in pancreatic ductal adenocarcinoma. Proteomics. 2003; 3(10):1988-2001. DOI: 10.1002/pmic.200300466. View

15.

Garrido C, Mehlen P, Fromentin A, Hammann A, Assem M, Arrigo A . Inconstant association between 27-kDa heat-shock protein (Hsp27) content and doxorubicin resistance in human colon cancer cells. The doxorubicin-protecting effect of Hsp27. Eur J Biochem. 1996; 237(3):653-9. DOI: 10.1111/j.1432-1033.1996.0653p.x. View

16.

Katagiri C, Nakanishi J, Kadoya K, Hibino T . Serpin squamous cell carcinoma antigen inhibits UV-induced apoptosis via suppression of c-JUN NH2-terminal kinase. J Cell Biol. 2006; 172(7):983-90. PMC: 2063756. DOI: 10.1083/jcb.200508064. View

17.

Ciocca D, Vargas-Roig L . Hsp27 as a prognostic and predictive factor in cancer. Prog Mol Subcell Biol. 2002; 28:205-18. DOI: 10.1007/978-3-642-56348-5_11. View

18.

Rocchi P, So A, Kojima S, Signaevsky M, Beraldi E, Fazli L . Heat shock protein 27 increases after androgen ablation and plays a cytoprotective role in hormone-refractory prostate cancer. Cancer Res. 2004; 64(18):6595-602. DOI: 10.1158/0008-5472.CAN-03-3998. View

19.

Glen A, Gan C, Hamdy F, Eaton C, Cross S, Catto J . iTRAQ-facilitated proteomic analysis of human prostate cancer cells identifies proteins associated with progression. J Proteome Res. 2008; 7(3):897-907. DOI: 10.1021/pr070378x. View

20.

Ito Y, Arai K, Ryushi , Nozawa , Yoshida H, Tomoda C . S100A9 expression is significantly linked to dedifferentiation of thyroid carcinoma. Pathol Res Pract. 2005; 201(8-9):551-6. DOI: 10.1016/j.prp.2005.08.003. View