Promoting Crystallisation of the Salmonella Enteritidis Fimbriae 14 Pilin SefD Using Deuterium Oxide

Overview

Journal Biochem Biophys Res Commun

Publisher Elsevier

Specialty Biochemistry

Date 2012 Apr 14

PMID 22497887

Citations 3

Authors

Bing Liu

James A Garnett

Wei-Chao Lee

Jing Lin

Paula Salgado

Jonathan Taylor

Yingqi Xu

Sebastian Lambert

Ernesto Cota

Steve Matthews

Affiliations

Soon will be listed here.

Abstract

The use of heavy water (D(2)O) as a solvent is commonplace in many spectroscopic techniques for the study of biological macromolecules. A significant deuterium isotope effect exists where hydrogen-bonding is important, such as in protein stability, dynamics and assembly. Here we illustrate the use of D(2)O in additive screening for the production of reproducible diffraction-quality crystals for the Salmonella enteritidis fimbriae 14 (SEF14) putative tip adhesin, SefD.

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