Experimental Model of Biofilm Implant-related Osteomyelitis to Test Combination Biomaterials Using Biofilms As Initial Inocula

Overview

Journal J Biomed Mater Res A

Specialty Biomedical Engineering

Date 2012 Apr 12

PMID 22492534

Citations 21

Authors

Dustin L Williams

Bryan S Haymond

Kassie L Woodbury

J Peter Beck

David E Moore

R Tyler Epperson

Roy D Bloebaum

Affiliations

Soon will be listed here.

Abstract

Currently, the majority of animal models that are used to study biofilm-related infections use planktonic bacterial cells as initial inocula to produce positive signals of infection in biomaterials studies. However, the use of planktonic cells has potentially led to inconsistent results in infection outcomes. In this study, well-established biofilms of methicillin-resistant Staphylococcus aureus were grown and used as initial inocula in an animal model of a Type IIIB open fracture. The goal of the work was to establish, for the first time, a repeatable model of biofilm implant-related osteomyelitis, wherein biofilms were used as initial inocula to test combination biomaterials. Results showed that 100% of animals that were treated with biofilms developed osteomyelitis, whereas 0% of animals not treated with biofilm developed infection. The development of this experimental model may lead to an important shift in biofilm and biomaterials research by showing that when biofilms are used as initial inocula, they may provide additional insights into how biofilm-related infections in the clinic develop and how they can be treated with combination biomaterials to eradicate and/or prevent biofilm formation.

Citing Articles

In vivo efficacy of a refillable intrawound drug delivery device in a sheep model of biofilm-compromised open fracture-related infection.

Williams D, Rothberg D, Kay W, Nehring L, Falconer R, Epperson R Biofilm. 2025; 9:100262.

PMID: 40059987 PMC: 11889977. DOI: 10.1016/j.bioflm.2025.100262.

The potential of sheep in preclinical models for bone infection research - A systematic review.

Beagan M, Dreyer C, Jensen L, Jensen H, Andersen T, Overgaard S J Orthop Translat. 2024; 45:120-131.

PMID: 38524868 PMC: 10960093. DOI: 10.1016/j.jot.2024.02.002.

Development and validation of a large animal ovine model for implant-associated spine infection using biofilm based inocula.

Shaw J, Bailey T, Ong J, Brodke D, Williams D, Wawrose R Biofilm. 2023; 6:100138.

PMID: 38078060 PMC: 10704336. DOI: 10.1016/j.bioflm.2023.100138.

Physical Approaches to Prevent and Treat Bacterial Biofilm.

Ciarolla A, Lapin N, Williams D, Chopra R, Greenberg D Antibiotics (Basel). 2023; 12(1).

PMID: 36671255 PMC: 9854850. DOI: 10.3390/antibiotics12010054.

Articular fragment restoration is critical to mitigate post-traumatic osteoarthritis in a porcine pilon fracture model.

DeKeyser G, Epperson R, Zhang C, Williams D, Olsen A, Haller J Osteoarthr Cartil Open. 2022; 4(2):100266.

PMID: 36475289 PMC: 9718277. DOI: 10.1016/j.ocarto.2022.100266.

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