Purification and Characterization of the Acetone Carboxylase of Cupriavidus Metallidurans Strain CH34

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 2012 Apr 12

PMID 22492439

Citations 4

Authors

Caroline Rosier

Natalie Leys

Celine Henoumont

Max Mergeay

Ruddy Wattiez

Affiliations

Soon will be listed here.

Abstract

Acetone carboxylase (Acx) is a key enzyme involved in the biodegradation of acetone by bacteria. Except for the Helicobacteraceae family, genome analyses revealed that bacteria that possess an Acx, such as Cupriavidus metallidurans strain CH34, are associated with soil. The Acx of CH34 forms the heterohexameric complex α(2)β(2)γ(2) and can carboxylate only acetone and 2-butanone in an ATP-dependent reaction to acetoacetate and 3-keto-2-methylbutyrate, respectively.

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