Identification and Characterization of Novel Perivascular Adventitial Cells in the Whole Mount Mesenteric Branch Artery Using Immunofluorescent Staining and Scanning Confocal Microscopy Imaging

Overview

Journal Int J Cell Biol

Publisher Wiley

Specialty Cell Biology

Date 2012 Apr 7

PMID 22481943

Authors

Chandra Somasundaram

Rahul K Nath

Richard D Bukoski

Debra I Diz

Affiliations

Soon will be listed here.

Abstract

A novel perivascular adventitial cell termed, adventitial neuronal somata (ANNIES) expressing the neural cell adhesion molecule (NCAM) and the vasodilator neuropeptide, calcitonin gene-related peptide (CGRP), exists in the adult rat mesenteric branch artery (MBA) in situ. In addition, we have previously shown that ANNIES coexpress CGRP and NCAM. We now show that ANNIES express the neurite growth marker, growth associated protein-43(Gap-43), palladin, and the calcium sensing receptor (CaSR), that senses changes in extracellular Ca(2+) and participates in vasodilator mechanisms. Thus, a previously characterized vasodilator, calcium sensing autocrine/paracrine system, exists in the perivascular adventitia associated with neural-vascular interface. Images of the whole mount MBA segments were analyzed under scanning confocal microscopy. Confocal analysis showed that the Gap-43, CaSR, and palladin were present in ANNIES about 37 ± 4%, 94 ± 6%, and 80 ± 10% respectively, comparable to CGRP (100%). Immunoblots from MBA confirmed the presence of Gap-43 (48 kD), NCAM (120 and 140 kD), and palladin (90-92 and 140 kD). In summary, CGRP, and NCAM-containing neural cells in the perivascular adventitia also express palladin and CaSR, and coexpress Gap-43 which may participate in response to stress/injury and vasodilator mechanisms as part of a perivascular sensory neural network.

References

Shen Y, Mani S, Donovan S, Schwob J, Meiri K . Growth-associated protein-43 is required for commissural axon guidance in the developing vertebrate nervous system. J Neurosci. 2002; 22(1):239-47. PMC: 6757587. View

Goicoechea S, Arneman D, Otey C . The role of palladin in actin organization and cell motility. Eur J Cell Biol. 2008; 87(8-9):517-25. PMC: 2597190. DOI: 10.1016/j.ejcb.2008.01.010. View

Zengin E, Chalajour F, Gehling U, Ito W, Treede H, Lauke H . Vascular wall resident progenitor cells: a source for postnatal vasculogenesis. Development. 2006; 133(8):1543-51. DOI: 10.1242/dev.02315. View

van der Loo B, Martin J . The adventitia, endothelium and atherosclerosis. Int J Microcirc Clin Exp. 1997; 17(5):280-8. DOI: 10.1159/000179240. View

Harno E, Edwards G, Geraghty A, Ward D, Dodd R, Dauban P . Evidence for the presence of GPRC6A receptors in rat mesenteric arteries. Cell Calcium. 2008; 44(2):210-9. DOI: 10.1016/j.ceca.2007.11.011. View

Crisan M, Yap S, Casteilla L, Chen C, Corselli M, Park T . A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell. 2008; 3(3):301-13. DOI: 10.1016/j.stem.2008.07.003. View

Tavian M, Zheng B, Oberlin E, Crisan M, Sun B, Huard J . The vascular wall as a source of stem cells. Ann N Y Acad Sci. 2005; 1044:41-50. DOI: 10.1196/annals.1349.006. View

Mechsner S, Schwarz J, Thode J, Loddenkemper C, Salomon D, Ebert A . Growth-associated protein 43-positive sensory nerve fibers accompanied by immature vessels are located in or near peritoneal endometriotic lesions. Fertil Steril. 2007; 88(3):581-7. DOI: 10.1016/j.fertnstert.2006.12.087. View

Corselli M, Chen C, Crisan M, Lazzari L, Peault B . Perivascular ancestors of adult multipotent stem cells. Arterioscler Thromb Vasc Biol. 2010; 30(6):1104-9. DOI: 10.1161/ATVBAHA.109.191643. View

10.

Toth C, Willis D, Twiss J, Walsh S, Martinez J, Liu W . Locally synthesized calcitonin gene-related Peptide has a critical role in peripheral nerve regeneration. J Neuropathol Exp Neurol. 2009; 68(3):326-37. PMC: 5790422. DOI: 10.1097/NEN.0b013e31819ac71b. View

11.

Smajilovic S, Hansen J, Christoffersen T, Lewin E, Sheikh S, Terwilliger E . Extracellular calcium sensing in rat aortic vascular smooth muscle cells. Biochem Biophys Res Commun. 2006; 348(4):1215-23. DOI: 10.1016/j.bbrc.2006.07.192. View

12.

Crisan M, Chen C, Corselli M, Andriolo G, Lazzari L, Peault B . Perivascular multipotent progenitor cells in human organs. Ann N Y Acad Sci. 2009; 1176:118-23. DOI: 10.1111/j.1749-6632.2009.04967.x. View

13.

Klein D, Hohn H, Kleff V, Tilki D, Ergun S . Vascular wall-resident stem cells. Histol Histopathol. 2010; 25(5):681-9. DOI: 10.14670/HH-25.681. View

14.

He Q, Dent E, Meiri K . Modulation of actin filament behavior by GAP-43 (neuromodulin) is dependent on the phosphorylation status of serine 41, the protein kinase C site. J Neurosci. 1997; 17(10):3515-24. PMC: 6573702. View

15.

Arribas S, Daly C, Gonzalez M, McGrath J . Imaging the vascular wall using confocal microscopy. J Physiol. 2007; 584(Pt 1):5-9. PMC: 2277073. DOI: 10.1113/jphysiol.2007.137786. View

16.

Parast M, Otey C . Characterization of palladin, a novel protein localized to stress fibers and cell adhesions. J Cell Biol. 2000; 150(3):643-56. PMC: 2175193. DOI: 10.1083/jcb.150.3.643. View

17.

Ronty M, Leivonen S, Hinz B, Rachlin A, Otey C, Kahari V . Isoform-specific regulation of the actin-organizing protein palladin during TGF-beta1-induced myofibroblast differentiation. J Invest Dermatol. 2006; 126(11):2387-96. DOI: 10.1038/sj.jid.5700427. View

18.

Crisan M, Huard J, Zheng B, Sun B, Yap S, Logar A . Purification and culture of human blood vessel-associated progenitor cells. Curr Protoc Stem Cell Biol. 2008; Chapter 2:Unit 2B.2.1-2B.2.13. DOI: 10.1002/9780470151808.sc02b02s4. View

19.

Tsai S, Yang L, Wu C, Chang S, Hsu C, Wei C . Injury-induced Janus kinase/protein kinase C-dependent phosphorylation of growth-associated protein 43 and signal transducer and activator of transcription 3 for neurite growth in dorsal root ganglion. J Neurosci Res. 2006; 85(2):321-31. DOI: 10.1002/jnr.21119. View

20.

Jin L, Gan Q, Zieba B, Goicoechea S, Owens G, Otey C . The actin associated protein palladin is important for the early smooth muscle cell differentiation. PLoS One. 2010; 5(9):e12823. PMC: 2943901. DOI: 10.1371/journal.pone.0012823. View