Esophageal Squamous Cell Carcinoma: Assessing Tumor Angiogenesis Using Multi-slice CT Perfusion Imaging

Overview

Journal Dig Dis Sci

Specialty Gastroenterology

Date 2012 Apr 6

PMID 22476585

Citations 3

Authors

Tao Song

Yu-Guang Shen

Na-Na Jiao

Xin-Hui Li

Hong-Tao Hu

Jin-Rong Qu

Xue-Jun Chen

Wen Feng

Xun Zhang

Hai-Liang Li

Affiliations

Soon will be listed here.

Abstract

Objectives: The purpose of this study was to investigate the correlation between multi-slice computed tomographic perfusion imaging (CTPI) parameters and immunohistologic markers of angiogenesis in esophageal squamous cell carcinoma (ESCC).

Methods: Fifty patients with histologically proven esophageal squamous cell carcinoma were enrolled in this study. All subjects underwent multi-slice CT perfusion scan. The hemodynamic parameters of vascular tumor, including blood volume (BV), blood flow (BF), mean transit time (MTT) and permeability surface (PS) were generated. All the ESCC specimens were stained immunohistochemically to identify CD31 for quantification of microvessel density (MVD). CTPI parameters were correlated with MVD by using Pearson correlation analysis.

Results: The value of CT perfusion parameters of ESCC were as follows: BF 116.71 ± 47.59 ml/100 g/min, BV 6.74 ± 2.70 ml/100 g, MTT 6.42 ± 2.84 s, PS 13.82 ± 6.25 ml/100 g/min. The mean MVD of all 50 tumor specimens was 34.44 ± 19.75. The PS values were significantly higher in ESCC patients with involvement of lymph node than those without involvement of lymph node (p < 0.01). Blood volume and permeability surface were positively correlated with MVD (p < 0.01), whereas no significant correlation was observed between MVD and BF or between MVD and MTT.

Conclusions: Blood volume and permeability surface were positively correlated with MVD. CTPI could reflect the angiogenesis in ESCC.

Citing Articles

Assessing Microcirculation in Resectable Oesophageal Squamous Cell Carcinoma with Dynamic Contrast-enhanced MRI for Identifying Primary tumour and Lymphatic Metastasis.

Chen Y, Jiang Y, Chen T, Li R, Zhang X, Chen F Sci Rep. 2019; 9(1):124.

PMID: 30644415 PMC: 6333778. DOI: 10.1038/s41598-018-36929-5.

Predicting the Response of Neoadjuvant Therapy for Patients with Esophageal Carcinoma: an In-depth Literature Review.

Tao C, Lin G, Xu Y, Mao W J Cancer. 2015; 6(11):1179-86.

PMID: 26516367 PMC: 4615355. DOI: 10.7150/jca.12346.

Computed tomography (CT) perfusion as an early predictive marker for treatment response to neoadjuvant chemotherapy in gastroesophageal junction cancer and gastric cancer--a prospective study.

Hansen M, Fallentin E, Lauridsen C, Law I, Federspiel B, Baeksgaard L PLoS One. 2014; 9(5):e97605.

PMID: 24845062 PMC: 4028233. DOI: 10.1371/journal.pone.0097605.

References

Cuenod C, Fournier L, Balvay D, Guinebretiere J . Tumor angiogenesis: pathophysiology and implications for contrast-enhanced MRI and CT assessment. Abdom Imaging. 2006; 31(2):188-93. DOI: 10.1007/s00261-005-0386-5. View

Weidner N, Semple J, Welch W, Folkman J . Tumor angiogenesis and metastasis--correlation in invasive breast carcinoma. N Engl J Med. 1991; 324(1):1-8. DOI: 10.1056/NEJM199101033240101. View

Lucchi M, Fontanini G, Mussi A, Vignati S, Ribechini A, Menconi G . Tumor angiogenesis and biologic markers in resected stage I NSCLC. Eur J Cardiothorac Surg. 1997; 12(4):535-41. DOI: 10.1016/s1010-7940(97)00218-2. View

El-Gohary Y, Metwally G, Saad R, Robinson M, Mesko T, Poppiti R . Prognostic significance of intratumoral and peritumoral lymphatic density and blood vessel density in invasive breast carcinomas. Am J Clin Pathol. 2008; 129(4):578-86. DOI: 10.1309/2HGNJ1GU57JMBJAQ. View

Provenzale J . Imaging of angiogenesis: clinical techniques and novel imaging methods. AJR Am J Roentgenol. 2006; 188(1):11-23. DOI: 10.2214/AJR.06.0280. View

Chen Y, Zhang J, Dai J, Feng X, Lu H, Zhou C . Angiogenesis of renal cell carcinoma: perfusion CT findings. Abdom Imaging. 2009; 35(5):622-8. DOI: 10.1007/s00261-009-9565-0. View

Privratsky J, Newman D, Newman P . PECAM-1: conflicts of interest in inflammation. Life Sci. 2010; 87(3-4):69-82. PMC: 2917326. DOI: 10.1016/j.lfs.2010.06.001. View

Greenstein A, Litle V, Swanson S, Divino C, Packer S, Wisnivesky J . Effect of the number of lymph nodes sampled on postoperative survival of lymph node-negative esophageal cancer. Cancer. 2008; 112(6):1239-46. DOI: 10.1002/cncr.23309. View

Fox S, Harris A . Histological quantitation of tumour angiogenesis. APMIS. 2004; 112(7-8):413-30. DOI: 10.1111/j.1600-0463.2004.apm11207-0803.x. View

10.

El-Shahat M, Lotfy M, Fahmy L, Abouel-Nour M, El-Kenawy A . Prognostic value of microvessel density, matrix metalloproteinase-9 and p53 protein expression in esophageal cancer. J Egypt Natl Canc Inst. 2005; 16(4):224-30. View

11.

Bellomi M, Viotti S, Preda L, DAndrea G, Bonello L, Petralia G . Perfusion CT in solid body-tumours. Part II: Clinical applications and future development. Radiol Med. 2010; 115(6):858-74. DOI: 10.1007/s11547-010-0545-9. View

12.

Woodfin A, Voisin M, Nourshargh S . PECAM-1: a multi-functional molecule in inflammation and vascular biology. Arterioscler Thromb Vasc Biol. 2007; 27(12):2514-23. DOI: 10.1161/ATVBAHA.107.151456. View

13.

Abdalla S, Behzad F, Bsharah S, Kumar S, Amini S, ODwyer S . Prognostic relevance of microvessel density in colorectal tumours. Oncol Rep. 1999; 6(4):839-42. DOI: 10.3892/or.6.4.839. View

14.

Verheul H, Voest E, Schlingemann R . Are tumours angiogenesis-dependent?. J Pathol. 2003; 202(1):5-13. DOI: 10.1002/path.1473. View

15.

Pircher A, Medinger M, Drevs J . Liver cancer: Targeted future options. World J Hepatol. 2011; 3(2):38-44. PMC: 3060418. DOI: 10.4254/wjh.v3.i2.38. View

16.

West C, Cooper R, Loncaster J, Wilks D, Bromley M . Tumor vascularity: a histological measure of angiogenesis and hypoxia. Cancer Res. 2001; 61(7):2907-10. View

17.

Kupisz K, CHIBOWSKI D, Klatka J, Klonowski S, Stepulak A . Tumor angiogenesis in patients with laryngeal cancer. Eur Arch Otorhinolaryngol. 1999; 256(6):303-5. DOI: 10.1007/s004050050251. View

18.

Li J, Zhao D, Jiang H, Huang Y, Li D, Wan Y . Assessment of tumor vascularization with functional computed tomography perfusion imaging in patients with cirrhotic liver disease. Hepatobiliary Pancreat Dis Int. 2011; 10(1):43-9. DOI: 10.1016/s1499-3872(11)60006-4. View

19.

Ash L, Teknos T, Gandhi D, Patel S, Mukherji S . Head and neck squamous cell carcinoma: CT perfusion can help noninvasively predict intratumoral microvessel density. Radiology. 2009; 251(2):422-8. DOI: 10.1148/radiol.2512080743. View

20.

Elpek G, Gelen T, Aksoy N, Erdogan A, Dertsiz L, Demircan A . The prognostic relevance of angiogenesis and mast cells in squamous cell carcinoma of the oesophagus. J Clin Pathol. 2001; 54(12):940-4. PMC: 1731336. DOI: 10.1136/jcp.54.12.940. View