Proteomics Analysis of Differentially Expressed Proteins in Chicken Trachea and Kidney After Infection with the Highly Virulent and Attenuated Coronavirus Infectious Bronchitis Virus in Vivo

Overview

Journal Proteome Sci

Publisher Biomed Central

Date 2012 Apr 3

PMID 22463732

Citations 19

Authors

Zhongzan Cao

Zongxi Han

Yuhao Shao

Xiaoli Liu

Junfeng Sun

Demin Yu

Xiangang Kong

Shengwang Liu

Affiliations

Soon will be listed here.

Abstract

Background: Infectious bronchitis virus (IBV) is first to be discovered coronavirus which is probably endemic in all regions with intensive impact on poultry production. In this study, we used two-dimensional gel electrophoresis (2-DE) and two-dimensional fluorescence difference gel electrophoresis (2-DIGE), coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS), to explore the global proteome profiles of trachea and kidney tissues from chicken at different stages infected in vivo with the highly virulent ck/CH/LDL/97I P5 strain of infectious bronchitis virus (IBV) and the embryo-passaged, attenuated ck/CH/LDL/97I P115 strain.

Results: Fifty-eight differentially expressed proteins were identified. Results demonstrated that some proteins which had functions in cytoskeleton organization, anti-oxidative stress, and stress response, showed different change patterns in abundance from chicken infected with the highly virulent ck/CH/LDL/97I P5 strain and those given the embryo-passaged, attenuated P115 stain. In addition, the dynamic transcriptional alterations of 12 selected proteins were analyzed by the real-time RT-PCR, and western blot analysis confirmed the change in abundance of heat shock proteins (HSP) beta-1, annexin A2, and annexin A5.

Conclusions: The proteomic alterations described here may suggest that these changes to protein expression correlate with IBV virus' virulence in chicken, hence provides valuable insights into the interactions of IBV with its host and may also assist with investigations of the pathogenesis of IBV and other coronavirus infections.

Citing Articles

Coronavirus and the Cytoskeleton of Virus-Infected Cells.

Xing Y, Zhang Q, Jiu Y Subcell Biochem. 2023; 106:333-364.

PMID: 38159233 DOI: 10.1007/978-3-031-40086-5_12.

Hyperthermia Enhances Adeno-Associated Virus Vector Transduction Efficiency in Melanoma Cells.

Bienkowska-Tokarczyk A, Stelmaszczyk-Emmel A, Demkow U, Malecki M Curr Issues Mol Biol. 2023; 45(10):8519-8538.

PMID: 37886980 PMC: 10604982. DOI: 10.3390/cimb45100537.

Coronavirus: proteomics analysis of chicken kidney tissue infected with variant 2 (IS-1494)-like avian infectious bronchitis virus.

Abdollahi H, Rezaei-Tavirani M, Ghalyanchilangeroudi A, Maghsoudloo H, Hashemzadeh M, Hosseini H Arch Virol. 2020; 166(1):101-113.

PMID: 33083914 PMC: 7574675. DOI: 10.1007/s00705-020-04845-7.

Antiviral activity of a novel mixture of natural antimicrobials, in vitro, and in a chicken infection model in vivo.

Balta I, Stef L, Pet I, Ward P, Callaway T, Ricke S Sci Rep. 2020; 10(1):16631.

PMID: 33024252 PMC: 7538884. DOI: 10.1038/s41598-020-73916-1.

Cytoskeleton-a crucial key in host cell for coronavirus infection.

Wen Z, Zhang Y, Lin Z, Shi K, Jiu Y J Mol Cell Biol. 2020; 12(12):968-979.

PMID: 32717049 PMC: 7454755. DOI: 10.1093/jmcb/mjaa042.

References

Martin S, Mohanty B, Cash P, Houlihan D, Secombes C . Proteome analysis of the Atlantic salmon (Salmo salar) cell line SHK-1 following recombinant IFN-gamma stimulation. Proteomics. 2007; 7(13):2275-86. DOI: 10.1002/pmic.200700020. View

Conrads K, Yi M, Simpson K, Lucas D, Camalier C, Yu L . A combined proteome and microarray investigation of inorganic phosphate-induced pre-osteoblast cells. Mol Cell Proteomics. 2005; 4(9):1284-96. DOI: 10.1074/mcp.M500082-MCP200. View

Harrist A, Ryzhova E, Harvey T, Gonzalez-Scarano F . Anx2 interacts with HIV-1 Gag at phosphatidylinositol (4,5) bisphosphate-containing lipid rafts and increases viral production in 293T cells. PLoS One. 2009; 4(3):e5020. PMC: 2657825. DOI: 10.1371/journal.pone.0005020. View

Maynard N, Gutschow M, Birch E, Covert M . The virus as metabolic engineer. Biotechnol J. 2010; 5(7):686-94. PMC: 3004434. DOI: 10.1002/biot.201000080. View

Vester D, Rapp E, Kluge S, Genzel Y, Reichl U . Virus-host cell interactions in vaccine production cell lines infected with different human influenza A virus variants: a proteomic approach. J Proteomics. 2010; 73(9):1656-69. DOI: 10.1016/j.jprot.2010.04.006. View

LeBouder F, Morello E, Rimmelzwaan G, Bosse F, Pechoux C, Delmas B . Annexin II incorporated into influenza virus particles supports virus replication by converting plasminogen into plasmin. J Virol. 2008; 82(14):6820-8. PMC: 2446977. DOI: 10.1128/JVI.00246-08. View

Liu C, Xu H, Liu D . Induction of caspase-dependent apoptosis in cultured cells by the avian coronavirus infectious bronchitis virus. J Virol. 2001; 75(14):6402-9. PMC: 114363. DOI: 10.1128/JVI.75.14.6402-6409.2001. View

Ryzhova E, Vos R, Albright A, Harrist A, Harvey T, Gonzalez-Scarano F . Annexin 2: a novel human immunodeficiency virus type 1 Gag binding protein involved in replication in monocyte-derived macrophages. J Virol. 2006; 80(6):2694-704. PMC: 1395445. DOI: 10.1128/JVI.80.6.2694-2704.2006. View

Perlman S, Netland J . Coronaviruses post-SARS: update on replication and pathogenesis. Nat Rev Microbiol. 2009; 7(6):439-50. PMC: 2830095. DOI: 10.1038/nrmicro2147. View

10.

Haq K, Brisbin J, Thanthrige-Don N, Heidari M, Sharif S . Transcriptome and proteome profiling of host responses to Marek's disease virus in chickens. Vet Immunol Immunopathol. 2010; 138(4):292-302. DOI: 10.1016/j.vetimm.2010.10.007. View

11.

Zhang H, Guo X, Ge X, Chen Y, Sun Q, Yang H . Changes in the cellular proteins of pulmonary alveolar macrophage infected with porcine reproductive and respiratory syndrome virus by proteomics analysis. J Proteome Res. 2009; 8(6):3091-7. DOI: 10.1021/pr900002f. View

12.

Vogels M, van Balkom B, Kaloyanova D, Batenburg J, Heck A, Helms J . Identification of host factors involved in coronavirus replication by quantitative proteomics analysis. Proteomics. 2010; 11(1):64-80. PMC: 7167679. DOI: 10.1002/pmic.201000309. View

13.

Beaton A, Rodriguez J, Reddy Y, Roy P . The membrane trafficking protein calpactin forms a complex with bluetongue virus protein NS3 and mediates virus release. Proc Natl Acad Sci U S A. 2002; 99(20):13154-9. PMC: 130602. DOI: 10.1073/pnas.192432299. View

14.

Wang X, Rosa A, Oliverira H, Rosa G, Guo X, Travnicek M . Transcriptome of local innate and adaptive immunity during early phase of infectious bronchitis viral infection. Viral Immunol. 2007; 19(4):768-74. DOI: 10.1089/vim.2006.19.768. View

15.

Lai C, Jou M, Huang S, Li S, Wan L, Tsai F . Proteomic analysis of up-regulated proteins in human promonocyte cells expressing severe acute respiratory syndrome coronavirus 3C-like protease. Proteomics. 2007; 7(9):1446-60. PMC: 7167764. DOI: 10.1002/pmic.200600459. View

16.

Emmott E, Smith C, Emmett S, Dove B, Hiscox J . Elucidation of the avian nucleolar proteome by quantitative proteomics using SILAC and changes in cells infected with the coronavirus infectious bronchitis virus. Proteomics. 2010; 10(19):3558-62. PMC: 7167976. DOI: 10.1002/pmic.201000139. View

17.

van Diepen A, Brand H, Sama I, Lambooy L, van den Heuvel L, van der Well L . Quantitative proteome profiling of respiratory virus-infected lung epithelial cells. J Proteomics. 2010; 73(9):1680-93. DOI: 10.1016/j.jprot.2010.04.008. View

18.

Li F, Tam J, Liu D . Cell cycle arrest and apoptosis induced by the coronavirus infectious bronchitis virus in the absence of p53. Virology. 2007; 365(2):435-45. PMC: 7103336. DOI: 10.1016/j.virol.2007.04.015. View

19.

Jiang X, Tang L, Dai J, Zhou H, Li S, Xia Q . Quantitative analysis of severe acute respiratory syndrome (SARS)-associated coronavirus-infected cells using proteomic approaches: implications for cellular responses to virus infection. Mol Cell Proteomics. 2005; 4(7):902-13. PMC: 7780044. DOI: 10.1074/mcp.M400112-MCP200. View

20.

Tang B, Chan K, Cheng V, Woo P, Lau S, Lam C . Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E. J Virol. 2005; 79(10):6180-93. PMC: 1091719. DOI: 10.1128/JVI.79.10.6180-6193.2005. View