Characterisation of Connective Tissue from the Hypertrophic Skeletal Muscle of Myostatin Null Mice

Overview

Journal J Anat

Specialty Anatomy & Histology

Date 2012 Apr 3

PMID 22463481

Citations 10

Authors

Mohamed I Elashry

Henry Collins-Hooper

Sakthivel Vaiyapuri

Ketan Patel

Affiliations

Soon will be listed here.

Abstract

Myostatin is a potent inhibitor of muscle development. Genetic deletion of myostatin in mice results in muscle mass increase, with muscles often weighing three times their normal values. Contracting muscle transfers tension to skeletal elements through an elaborate connective tissue network. Therefore, the connective tissue of skeletal muscle is an integral component of the contractile apparatus. Here we examine the connective tissue architecture in myostatin null muscle. We show that the hypertrophic muscle has decreased connective tissue content compared with wild-type muscle. Secondly, we show that the hypertrophic muscle fails to show the normal increase in muscle connective tissue content during ageing. Therefore, genetic deletion of myostatin results in an increase in contractile elements but a decrease in connective tissue content. We propose a model based on the contractile profile of muscle fibres that reconciles this apparent incompatible tissue composition phenotype.

Citing Articles

Differential effects of the venoms of Russell's viper and Indian cobra on human myoblasts.

Haidar H, Almeida J, Williams J, Guo B, Bigot A, Senthilkumaran S Sci Rep. 2024; 14(1):3184.

PMID: 38326450 PMC: 10850160. DOI: 10.1038/s41598-024-53366-9.

Deletion of Annexin A1 in Mice Upregulates the Expression of Its Receptor, Fpr2/3, and Reactivity to the AnxA1 Mimetic Peptide in Platelets.

Zharkova O, Salamah M, Babak M, Rajan E, Lim L, Andrade F Int J Mol Sci. 2023; 24(4).

PMID: 36834844 PMC: 9962723. DOI: 10.3390/ijms24043424.

Myostatin deficiency decreases cardiac extracellular matrix in pigs.

Paek H, Quan B, Choe H, Li Z, Yin X Transgenic Res. 2022; 31(4-5):553-565.

PMID: 35978205 DOI: 10.1007/s11248-022-00322-w.

1,8-Cineole Affects Agonists-Induced Platelet Activation, Thrombus Formation and Haemostasis.

Alatawi K, Ravishankar D, Patra P, Bye A, Stainer A, Patel K Cells. 2021; 10(10).

PMID: 34685597 PMC: 8533741. DOI: 10.3390/cells10102616.

Exotic Snakebites Reported to Pennsylvania Poison Control Centers: Lessons Learned on the Demographics, Clinical Effects, and Treatment of These Cases.

Miller S, Osterhoudt K, Korenoski A, Patel K, Vaiyapuri S Toxins (Basel). 2020; 12(12).

PMID: 33260454 PMC: 7760318. DOI: 10.3390/toxins12120755.

References

Mendias C, Marcin J, Calerdon D, Faulkner J . Contractile properties of EDL and soleus muscles of myostatin-deficient mice. J Appl Physiol (1985). 2006; 101(3):898-905. PMC: 4088255. DOI: 10.1152/japplphysiol.00126.2006. View

Bogdanovich S, Krag T, Barton E, Morris L, Whittemore L, Ahima R . Functional improvement of dystrophic muscle by myostatin blockade. Nature. 2002; 420(6914):418-21. DOI: 10.1038/nature01154. View

Langley B, Thomas M, Bishop A, Sharma M, Gilmour S, Kambadur R . Myostatin inhibits myoblast differentiation by down-regulating MyoD expression. J Biol Chem. 2002; 277(51):49831-40. DOI: 10.1074/jbc.M204291200. View

Zhu J, Li Y, Shen W, Qiao C, Ambrosio F, Lavasani M . Relationships between transforming growth factor-beta1, myostatin, and decorin: implications for skeletal muscle fibrosis. J Biol Chem. 2007; 282(35):25852-63. DOI: 10.1074/jbc.M704146200. View

McLoon L . Focusing on fibrosis: halofuginone-induced functional improvement in the mdx mouse model of Duchenne muscular dystrophy. Am J Physiol Heart Circ Physiol. 2008; 294(4):H1505-7. DOI: 10.1152/ajpheart.00176.2008. View

Laurent G, Tetley T . Pulmonary fibrosis and emphysema: connective tissue disorders of the lung. Eur J Clin Invest. 1984; 14(6):411-3. DOI: 10.1111/j.1365-2362.1984.tb01204.x. View

Mendias C, Bakhurin K, Faulkner J . Tendons of myostatin-deficient mice are small, brittle, and hypocellular. Proc Natl Acad Sci U S A. 2007; 105(1):388-93. PMC: 2224222. DOI: 10.1073/pnas.0707069105. View

Schuelke M, Wagner K, Stolz L, Hubner C, Riebel T, Komen W . Myostatin mutation associated with gross muscle hypertrophy in a child. N Engl J Med. 2004; 350(26):2682-8. DOI: 10.1056/NEJMoa040933. View

McPherron A, Lawler A, Lee S . Regulation of skeletal muscle mass in mice by a new TGF-beta superfamily member. Nature. 1997; 387(6628):83-90. DOI: 10.1038/387083a0. View

10.

Kardon G, Harfe B, Tabin C . A Tcf4-positive mesodermal population provides a prepattern for vertebrate limb muscle patterning. Dev Cell. 2003; 5(6):937-44. DOI: 10.1016/s1534-5807(03)00360-5. View

11.

Fulzele S, Arounleut P, Cain M, Herberg S, Hunter M, Wenger K . Role of myostatin (GDF-8) signaling in the human anterior cruciate ligament. J Orthop Res. 2010; 28(8):1113-8. PMC: 3755889. DOI: 10.1002/jor.21112. View

12.

Shelton G, Engvall E . Gross muscle hypertrophy in whippet dogs is caused by a mutation in the myostatin gene. Neuromuscul Disord. 2007; 17(9-10):721-2. DOI: 10.1016/j.nmd.2007.06.008. View

13.

Wagner K, McPherron A, Winik N, Lee S . Loss of myostatin attenuates severity of muscular dystrophy in mdx mice. Ann Neurol. 2002; 52(6):832-6. DOI: 10.1002/ana.10385. View

14.

Srikanthan P, Karlamangla A . Relative muscle mass is inversely associated with insulin resistance and prediabetes. Findings from the third National Health and Nutrition Examination Survey. J Clin Endocrinol Metab. 2011; 96(9):2898-903. DOI: 10.1210/jc.2011-0435. View

15.

Zhang D, Liu M, Ding F, Gu X . Expression of myostatin RNA transcript and protein in gastrocnemius muscle of rats after sciatic nerve resection. J Muscle Res Cell Motil. 2006; 27(1):37-44. DOI: 10.1007/s10974-005-9050-5. View

16.

Bo Li Z, Kollias H, Wagner K . Myostatin directly regulates skeletal muscle fibrosis. J Biol Chem. 2008; 283(28):19371-8. PMC: 2443655. DOI: 10.1074/jbc.M802585200. View

17.

Kaariainen M, Jarvinen T, Jarvinen M, Rantanen J, Kalimo H . Relation between myofibers and connective tissue during muscle injury repair. Scand J Med Sci Sports. 2000; 10(6):332-7. DOI: 10.1034/j.1600-0838.2000.010006332.x. View

18.

Valasek P, Theis S, DeLaurier A, Hinits Y, Luke G, Otto A . Cellular and molecular investigations into the development of the pectoral girdle. Dev Biol. 2011; 357(1):108-16. DOI: 10.1016/j.ydbio.2011.06.031. View

19.

Kovanen V, Suominen H, Heikkinen E . Connective tissue of "fast" and "slow" skeletal muscle in rats--effects of endurance training. Acta Physiol Scand. 1980; 108(2):173-80. DOI: 10.1111/j.1748-1716.1980.tb06515.x. View

20.

Elashry M, Otto A, Matsakas A, El-Morsy S, Jones L, Anderson B . Axon and muscle spindle hyperplasia in the myostatin null mouse. J Anat. 2011; 218(2):173-84. PMC: 3042751. DOI: 10.1111/j.1469-7580.2010.01327.x. View