Purification and Cellular Localization of the Entamoeba Histolytica Transcarboxylase

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2012 Mar 29

PMID 22453500

Citations 2

Authors

E Barbosa-Cabrera

A Salas-Casas

S Rojas-Hernandez

A Jarillo-Luna

E Abarca-Rojano

M A Rodriguez

R Campos-Rodriguez

Affiliations

Soon will be listed here.

Abstract

Genome analysis of Entamoeba histolytica predicts the presence of acetyl-CoA carboxylase. Using Western blot, histochemistry, and confocal microscopy, we demonstrated the presence of a biotin-containing protein in the cytoplasm of E. histolytica, with a molecular weight of 136 kDa and biotin-carboxylase activity. This protein probably corresponds to a transcarboxylase that catalyzes the rate-limiting reaction leading to fatty acid elongation.

Citing Articles

The Mastigamoeba balamuthi Genome and the Nature of the Free-Living Ancestor of Entamoeba.

Zarsky V, Klimes V, Paces J, Vlcek C, Hradilova M, Benes V Mol Biol Evol. 2021; 38(6):2240-2259.

PMID: 33528570 PMC: 8136499. DOI: 10.1093/molbev/msab020.

: Quantitative Proteomics Analysis Reveals Putative Virulence-Associated Differentially Abundant Membrane Proteins.

Ng Y, Olivos-Garcia A, Lim T, Noordin R, Lin Q, Othman N Am J Trop Med Hyg. 2018; 99(6):1518-1529.

PMID: 30298805 PMC: 6283474. DOI: 10.4269/ajtmh.18-0415.

References

Guchhait R, Polakis S, Dimroth P, Stoll E, Moss J, Lane M . Acetyl coenzyme A carboxylase system of Escherichia coli. Purification and properties of the biotin carboxylase, carboxyltransferase, and carboxyl carrier protein components. J Biol Chem. 1974; 249(20):6633-45. View

Cho Y, Lee J, Shin D, Kim H, Cheon Y, Seo C . Crystal structure of the biotin carboxylase domain of human acetyl-CoA carboxylase 2. Proteins. 2007; 70(1):268-72. DOI: 10.1002/prot.21611. View

Tong L . Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery. Cell Mol Life Sci. 2005; 62(16):1784-803. PMC: 11139103. DOI: 10.1007/s00018-005-5121-4. View

Jakobsson A, Westerberg R, Jacobsson A . Fatty acid elongases in mammals: their regulation and roles in metabolism. Prog Lipid Res. 2006; 45(3):237-49. DOI: 10.1016/j.plipres.2006.01.004. View

Argarana C, Kuntz I, Birken S, Axel R, Cantor C . Molecular cloning and nucleotide sequence of the streptavidin gene. Nucleic Acids Res. 1986; 14(4):1871-82. PMC: 339579. DOI: 10.1093/nar/14.4.1871. View

Weber P, Cox M, Salemme F, Ohlendorf D . Crystallographic data for Streptomyces avidinii streptavidin. J Biol Chem. 1987; 262(26):12728-9. View

Reeves R . Phosphopyruvate carboxylase from entamoeba histolytica. Biochim Biophys Acta. 1970; 220(2):346-9. DOI: 10.1016/0005-2744(70)90021-5. View

Marinets A, Zhang T, Guillen N, Gounon P, Bohle B, Vollmann U . Protection against invasive amebiasis by a single monoclonal antibody directed against a lipophosphoglycan antigen localized on the surface of Entamoeba histolytica. J Exp Med. 1997; 186(9):1557-65. PMC: 2199119. DOI: 10.1084/jem.186.9.1557. View

Jelenska J, Sirikhachornkit A, Haselkorn R, Gornicki P . The carboxyltransferase activity of the apicoplast acetyl-CoA carboxylase of Toxoplasma gondii is the target of aryloxyphenoxypropionate inhibitors. J Biol Chem. 2002; 277(26):23208-15. DOI: 10.1074/jbc.M200455200. View

10.

Tehlivets O, Scheuringer K, Kohlwein S . Fatty acid synthesis and elongation in yeast. Biochim Biophys Acta. 2006; 1771(3):255-70. DOI: 10.1016/j.bbalip.2006.07.004. View

11.

McArthur A, Morrison H, Nixon J, Passamaneck N, Kim U, Hinkle G . The Giardia genome project database. FEMS Microbiol Lett. 2000; 189(2):271-3. DOI: 10.1111/j.1574-6968.2000.tb09242.x. View

12.

Lee S, Stephens J, Englund P . A fatty-acid synthesis mechanism specialized for parasitism. Nat Rev Microbiol. 2007; 5(4):287-97. DOI: 10.1038/nrmicro1617. View

13.

Zhu G . Current progress in the fatty acid metabolism in Cryptosporidium parvum. J Eukaryot Microbiol. 2004; 51(4):381-8. DOI: 10.1111/j.1550-7408.2004.tb00384.x. View

14.

Zuther E, Johnson J, Haselkorn R, McLeod R, Gornicki P . Growth of Toxoplasma gondii is inhibited by aryloxyphenoxypropionate herbicides targeting acetyl-CoA carboxylase. Proc Natl Acad Sci U S A. 1999; 96(23):13387-92. PMC: 23957. DOI: 10.1073/pnas.96.23.13387. View

15.

Das S, Stevens T, Castillo C, Villasenor A, Arredondo H, Reddy K . Lipid metabolism in mucous-dwelling amitochondriate protozoa. Int J Parasitol. 2002; 32(6):655-75. DOI: 10.1016/s0020-7519(02)00006-1. View

16.

Waller R, Ralph S, Reed M, Su V, Douglas J, Minnikin D . A type II pathway for fatty acid biosynthesis presents drug targets in Plasmodium falciparum. Antimicrob Agents Chemother. 2002; 47(1):297-301. PMC: 148988. DOI: 10.1128/AAC.47.1.297-301.2003. View

17.

Brownsey R, Boone A, Elliott J, Kulpa J, Lee W . Regulation of acetyl-CoA carboxylase. Biochem Soc Trans. 2006; 34(Pt 2):223-7. DOI: 10.1042/BST20060223. View

18.

Clark C, Alsmark U, Tazreiter M, Saito-Nakano Y, Ali V, Marion S . Structure and content of the Entamoeba histolytica genome. Adv Parasitol. 2007; 65:51-190. DOI: 10.1016/S0065-308X(07)65002-7. View

19.

Heath R, White S, Rock C . Inhibitors of fatty acid synthesis as antimicrobial chemotherapeutics. Appl Microbiol Biotechnol. 2002; 58(6):695-703. DOI: 10.1007/s00253-001-0918-z. View

20.

Shen Y, Volrath S, Weatherly S, Elich T, Tong L . A mechanism for the potent inhibition of eukaryotic acetyl-coenzyme A carboxylase by soraphen A, a macrocyclic polyketide natural product. Mol Cell. 2004; 16(6):881-91. DOI: 10.1016/j.molcel.2004.11.034. View