2011 Rita Schaffer Lecture: Nanoparticles for Intracellular Nucleic Acid Delivery

Overview

Journal Ann Biomed Eng

Specialty Biomedical Engineering

Date 2012 Mar 28

PMID 22451256

Citations 9

Authors

Jordan J Green

Affiliations

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Abstract

Nanoparticles are a promising technology for delivery of new types of therapeutics. A polymer library approach has allowed engineering of polymeric particles that are particularly effective for the delivery of DNA and siRNA to human cells. Certain chemical structural motifs, degradable linkages, hydrophobicity, and biophysical properties are key for successful intracellular delivery. Small differences to biomaterial structure, and especially the type of degradable linkage in the polymers, can be critical for successful delivery of siRNA vs. DNA. Furthermore, subtle changes to biomaterial structure can facilitate cell-type gene delivery specificity between human brain cancer cells and healthy cells as well as between human retinal endothelial cells and epithelial cells. These polymeric nanoparticles are effective for nucleic acid delivery in a broad range of human cell types and have applications to regenerative medicine, ophthalmology, and cancer among many other biomedical research areas.

Citing Articles

Polymeric nanoparticles for nonviral gene therapy extend brain tumor survival in vivo.

Mangraviti A, Tzeng S, Kozielski K, Wang Y, Jin Y, Gullotti D ACS Nano. 2015; 9(2):1236-49.

PMID: 25643235 PMC: 4342728. DOI: 10.1021/nn504905q.

Differential polymer structure tunes mechanism of cellular uptake and transfection routes of poly(β-amino ester) polyplexes in human breast cancer cells.

Kim J, Sunshine J, Green J Bioconjug Chem. 2013; 25(1):43-51.

PMID: 24320687 PMC: 4016154. DOI: 10.1021/bc4002322.

(3-aminopropyl)-4-methylpiperazine end-capped poly(1,4-butanediol diacrylate-co-4-amino-1-butanol)-based multilayer films for gene delivery.

Li C, Tzeng S, Tellier L, Green J ACS Appl Mater Interfaces. 2013; 5(13):5947-53.

PMID: 23755861 PMC: 3838882. DOI: 10.1021/am402115v.

The effect and role of carbon atoms in poly(β-amino ester)s for DNA binding and gene delivery.

Bishop C, Ketola T, Tzeng S, Sunshine J, Urtti A, Lemmetyinen H J Am Chem Soc. 2013; 135(18):6951-7.

PMID: 23570657 PMC: 3838887. DOI: 10.1021/ja4002376.

Student award winner in the Ph.D. category for the 2013 society for biomaterials annual meeting and exposition, april 10-13, 2013, Boston, Massachusetts : biomaterial-mediated cancer-specific DNA delivery to liver cell cultures using synthetic....

Tzeng S, Higgins L, Pomper M, Green J J Biomed Mater Res A. 2013; 101(7):1837-45.

PMID: 23559534 PMC: 3915782. DOI: 10.1002/jbm.a.34616.

References

Arote R, Jiang H, Kim Y, Cho M, Choi Y, Cho C . Degradable poly(amido amine)s as gene delivery carriers. Expert Opin Drug Deliv. 2011; 8(9):1237-46. DOI: 10.1517/17425247.2011.586333. View

Nguyen D, Green J, Chan J, Longer R, Anderson D . Polymeric Materials for Gene Delivery and DNA Vaccination. Adv Mater. 2017; 21(8):847-867. PMC: 5391878. DOI: 10.1002/adma.200801478. View

Huang Y, Zugates G, Peng W, Holtz D, Dunton C, Green J . Nanoparticle-delivered suicide gene therapy effectively reduces ovarian tumor burden in mice. Cancer Res. 2009; 69(15):6184-91. PMC: 2735403. DOI: 10.1158/0008-5472.CAN-09-0061. View

Sunshine J, Bhise N, Green J . Degradable polymers for gene delivery. Annu Int Conf IEEE Eng Med Biol Soc. 2009; 2009:2412-5. PMC: 3959118. DOI: 10.1109/IEMBS.2009.5334767. View

Merdan T, Kopecek J, Kissel T . Prospects for cationic polymers in gene and oligonucleotide therapy against cancer. Adv Drug Deliv Rev. 2002; 54(5):715-58. DOI: 10.1016/s0169-409x(02)00046-7. View

Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K . Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007; 131(5):861-72. DOI: 10.1016/j.cell.2007.11.019. View

Harris T, Green J, Fung P, Langer R, Anderson D, Bhatia S . Tissue-specific gene delivery via nanoparticle coating. Biomaterials. 2009; 31(5):998-1006. PMC: 2796451. DOI: 10.1016/j.biomaterials.2009.10.012. View

Watson J, Crick F . Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid. Nature. 1953; 171(4356):737-8. DOI: 10.1038/171737a0. View

Tzeng S, Guerrero-Cazares H, Martinez E, Sunshine J, Quinones-Hinojosa A, Green J . Non-viral gene delivery nanoparticles based on poly(β-amino esters) for treatment of glioblastoma. Biomaterials. 2011; 32(23):5402-10. PMC: 3118545. DOI: 10.1016/j.biomaterials.2011.04.016. View

10.

Putnam D . Polymers for gene delivery across length scales. Nat Mater. 2006; 5(6):439-51. DOI: 10.1038/nmat1645. View

11.

Acharya S, Sahoo S . PLGA nanoparticles containing various anticancer agents and tumour delivery by EPR effect. Adv Drug Deliv Rev. 2010; 63(3):170-83. DOI: 10.1016/j.addr.2010.10.008. View

12.

Zhou J, Liu J, Cheng C, Patel T, Weller C, Piepmeier J . Biodegradable poly(amine-co-ester) terpolymers for targeted gene delivery. Nat Mater. 2011; 11(1):82-90. PMC: 4180913. DOI: 10.1038/nmat3187. View

13.

Mahato R . Water insoluble and soluble lipids for gene delivery. Adv Drug Deliv Rev. 2005; 57(5):699-712. DOI: 10.1016/j.addr.2004.12.005. View

14.

Verma I, Somia N . Gene therapy -- promises, problems and prospects. Nature. 1997; 389(6648):239-42. DOI: 10.1038/38410. View

15.

Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin I . Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009; 324(5928):797-801. PMC: 2758053. DOI: 10.1126/science.1172482. View

16.

Ogris M, Walker G, Blessing T, Kircheis R, Wolschek M, Wagner E . Tumor-targeted gene therapy: strategies for the preparation of ligand-polyethylene glycol-polyethylenimine/DNA complexes. J Control Release. 2003; 91(1-2):173-81. DOI: 10.1016/s0168-3659(03)00230-x. View

17.

Griffith O . Biologic and pharmacologic regulation of mammalian glutathione synthesis. Free Radic Biol Med. 1999; 27(9-10):922-35. DOI: 10.1016/s0891-5849(99)00176-8. View

18.

von Gersdorff K, Sanders N, Vandenbroucke R, De Smedt S, Wagner E, Ogris M . The internalization route resulting in successful gene expression depends on both cell line and polyethylenimine polyplex type. Mol Ther. 2006; 14(5):745-53. DOI: 10.1016/j.ymthe.2006.07.006. View

19.

Partridge K, Oreffo R . Gene delivery in bone tissue engineering: progress and prospects using viral and nonviral strategies. Tissue Eng. 2004; 10(1-2):295-307. DOI: 10.1089/107632704322791934. View

20.

Thomas C, Ehrhardt A, Kay M . Progress and problems with the use of viral vectors for gene therapy. Nat Rev Genet. 2003; 4(5):346-58. DOI: 10.1038/nrg1066. View