The ATM-BID Pathway Regulates Quiescence and Survival of Haematopoietic Stem Cells

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2012 Mar 27

PMID 22446738

Citations 76

Authors

Maria Maryanovich

Galia Oberkovitz

Hagit Niv

Lidiya Vorobiyov

Yehudit Zaltsman

Ori Brenner

Tsvee Lapidot

Steffen Jung

Atan Gross

Affiliations

Soon will be listed here.

Abstract

BID, a BH3-only BCL2 family member, functions in apoptosis as well as the DNA-damage response. Our previous data demonstrated that BID is an ATM effector acting to induce cell-cycle arrest and inhibition of apoptosis following DNA damage. Here we show that ATM-mediated BID phosphorylation plays an unexpected role in maintaining the quiescence of haematopoietic stem cells (HSCs). Loss of BID phosphorylation leads to escape from quiescence of HSCs, resulting in exhaustion of the HSC pool and a marked reduction of HSC repopulating potential in vivo. We also demonstrate that BID phosphorylation plays a role in protecting HSCs from irradiation, and that regulating both quiescence and survival of HSCs depends on BID's ability to regulate oxidative stress. Moreover, loss of BID phosphorylation, ATM knockout or exposing mice to irradiation leads to an increase in mitochondrial BID, which correlates with an increase in mitochondrial oxidative stress. These results show that the ATM-BID pathway serves as a critical checkpoint for coupling HSC homeostasis and the DNA-damage stress response to enable long-term regenerative capacity.

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