Acetylcholine Induces Mesenchymal Stem Cell Migration Via Ca2+ /PKC/ERK1/2 Signal Pathway

Overview

Journal J Cell Biochem

Specialties Biochemistry
Cell Biology

Date 2012 Mar 24

PMID 22441978

Citations 28

Authors

Jun-Ming Tang

Jie Yuan

Qing Li

Jia-Ning Wang

Xia Kong

Fei Zheng

Lei Zhang

Long Chen

Lin-Yun Guo

Yong-Hang Huang

Jian-Ye Yang

Shi-You Chen

Affiliations

Soon will be listed here.

Abstract

Acetylcholine (ACh) plays an important role in neural and non-neural function, but its role in mesenchymal stem cell (MSC) migration remains to be determined. In the present study, we have found that ACh induces MSC migration via muscarinic acetylcholine receptors (mAChRs). Among several mAChRs, MSCs express mAChR subtype 1 (m1AChR). ACh induces MSC migration via interaction with mAChR1. MEK1/2 inhibitor PD98059 blocks ERK1/2 phosphorylation while partially inhibiting the ACh-induced MSC migration. InsP3Rs inhibitor 2-APB that inhibits MAPK/ERK phosphorylation completely blocks ACh-mediated MSC migration. Interestingly, intracellular Ca(2+) ATPase-specific inhibitor thapsigargin also completely blocks ACh-induced MSC migration through the depletion of intracellular Ca(2+) storage. PKCα or PKCβ inhibitor or their siRNAs only partially inhibit ACh-induced MSC migration, but PKC-ζ siRNA completely inhibits ACh-induced MSC migration via blocking ERK1/2 phosphorylation. These results indicate that ACh induces MSC migration via Ca(2+), PKC, and ERK1/2 signal pathways.

Citing Articles

Exploring Memory Function Beyond Immune Cells: ANGPTL4-Mediated Memory Functions in Tissue Resident Stem Cells.

Park S, Min E, Kim S, Kim S, Na K, Park C Adv Sci (Weinh). 2024; 11(28):e2307545.

PMID: 38666393 PMC: 11267307. DOI: 10.1002/advs.202307545.

Unveiling the potential effects of acetylsalicylic acid: insights into regeneration in endometrial stem cells.

Park S, Kim S, Min E, Oh B, Jung Y, Kim Y Cell Commun Signal. 2023; 21(1):323.

PMID: 37950232 PMC: 10638813. DOI: 10.1186/s12964-023-01339-2.

ATP purinergic receptor signalling promotes Sca-1 cell proliferation and migration for vascular remodelling.

Cui Y, Li C, Zeng X, Wei X, Li P, Cheng J Cell Commun Signal. 2023; 21(1):173.

PMID: 37430253 PMC: 10332060. DOI: 10.1186/s12964-023-01185-2.

A novel role of follicle-stimulating hormone (FSH) in various regeneration-related functions of endometrial stem cells.

Park S, Kim S, Kim S, Park J, Hong I Exp Mol Med. 2022; 54(9):1524-1535.

PMID: 36117220 PMC: 9534881. DOI: 10.1038/s12276-022-00858-1.

Cell Therapy of Stroke: Do the Intra-Arterially Transplanted Mesenchymal Stem Cells Cross the Blood-Brain Barrier?.

Yarygin K, Namestnikova D, Sukhinich K, Gubskiy I, Majouga A, Kholodenko I Cells. 2021; 10(11).

PMID: 34831220 PMC: 8616541. DOI: 10.3390/cells10112997.

References

Kim M, Kim M, Heo J, Kim J, Han H . Acetylcholine inhibits long-term hypoxia-induced apoptosis by suppressing the oxidative stress-mediated MAPKs activation as well as regulation of Bcl-2, c-IAPs, and caspase-3 in mouse embryonic stem cells. Apoptosis. 2007; 13(2):295-304. DOI: 10.1007/s10495-007-0160-y. View

Yamboliev I, Wiesmann K, Singer C, Hedges J, Gerthoffer W . Phosphatidylinositol 3-kinases regulate ERK and p38 MAP kinases in canine colonic smooth muscle. Am J Physiol Cell Physiol. 2000; 279(2):C352-60. DOI: 10.1152/ajpcell.2000.279.2.C352. View

Kasenda B, Kassmer S, Niggemann B, Schiermeier S, Hatzmann W, Zanker K . The stromal cell-derived factor-1alpha dependent migration of human cord blood CD34 haematopoietic stem and progenitor cells switches from protein kinase C (PKC)-alpha dependence to PKC-alpha independence upon prolonged culture in the presence of.... Br J Haematol. 2008; 142(5):831-5. DOI: 10.1111/j.1365-2141.2008.07256.x. View

Paraoanu L, Steinert G, Koehler A, Wessler I, Layer P . Expression and possible functions of the cholinergic system in a murine embryonic stem cell line. Life Sci. 2007; 80(24-25):2375-9. DOI: 10.1016/j.lfs.2007.03.008. View

Fu X, Han B, Cai S, Lei Y, Sun T, Sheng Z . Migration of bone marrow-derived mesenchymal stem cells induced by tumor necrosis factor-alpha and its possible role in wound healing. Wound Repair Regen. 2009; 17(2):185-91. DOI: 10.1111/j.1524-475X.2009.00454.x. View

Kawano S, Shoji S, Ichinose S, Yamagata K, Tagami M, Hiraoka M . Characterization of Ca(2+) signaling pathways in human mesenchymal stem cells. Cell Calcium. 2002; 32(4):165-74. DOI: 10.1016/s0143416002001240. View

Serobyan N, Jagannathan S, Orlovskaya I, Schraufstatter I, Skok M, Loring J . The cholinergic system is involved in regulation of the development of the hematopoietic system. Life Sci. 2007; 80(24-25):2352-60. PMC: 2873871. DOI: 10.1016/j.lfs.2007.04.017. View

Resende R, Adhikari A . Cholinergic receptor pathways involved in apoptosis, cell proliferation and neuronal differentiation. Cell Commun Signal. 2009; 7:20. PMC: 2744676. DOI: 10.1186/1478-811X-7-20. View

Ridley A, Schwartz M, Burridge K, Firtel R, Ginsberg M, Borisy G . Cell migration: integrating signals from front to back. Science. 2003; 302(5651):1704-9. DOI: 10.1126/science.1092053. View

10.

de Jong D, Steegenga W, Hendriks J, van Zoelen E, Olijve W, Dechering K . Regulation of Notch signaling genes during BMP2-induced differentiation of osteoblast precursor cells. Biochem Biophys Res Commun. 2004; 320(1):100-7. DOI: 10.1016/j.bbrc.2004.05.150. View

11.

Ryu C, Park S, Kim S, Lim J, Jeong C, Jun J . Migration of human umbilical cord blood mesenchymal stem cells mediated by stromal cell-derived factor-1/CXCR4 axis via Akt, ERK, and p38 signal transduction pathways. Biochem Biophys Res Commun. 2010; 398(1):105-10. DOI: 10.1016/j.bbrc.2010.06.043. View

12.

Kim T, Seong J, Ouyang M, Sun J, Lu S, Hong J . Substrate rigidity regulates Ca2+ oscillation via RhoA pathway in stem cells. J Cell Physiol. 2008; 218(2):285-93. PMC: 2592496. DOI: 10.1002/jcp.21598. View

13.

Huang G, Gronthos S, Shi S . Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res. 2009; 88(9):792-806. PMC: 2830488. DOI: 10.1177/0022034509340867. View

14.

Tang J, Wang J, Kong X, Yang J, Guo L, Zheng F . Vascular endothelial growth factor promotes cardiac stem cell migration via the PI3K/Akt pathway. Exp Cell Res. 2009; 315(20):3521-31. DOI: 10.1016/j.yexcr.2009.09.026. View

15.

Chernyavsky A, Arredondo J, Wess J, Karlsson E, Grando S . Novel signaling pathways mediating reciprocal control of keratinocyte migration and wound epithelialization through M3 and M4 muscarinic receptors. J Cell Biol. 2004; 166(2):261-72. PMC: 2172302. DOI: 10.1083/jcb.200401034. View

16.

Bruder S, Fink D, Caplan A . Mesenchymal stem cells in bone development, bone repair, and skeletal regeneration therapy. J Cell Biochem. 1994; 56(3):283-94. PMC: 7166813. DOI: 10.1002/jcb.240560303. View

17.

Dempsey E, Newton A, Mochly-Rosen D, Fields A, Reyland M, Insel P . Protein kinase C isozymes and the regulation of diverse cell responses. Am J Physiol Lung Cell Mol Physiol. 2000; 279(3):L429-38. DOI: 10.1152/ajplung.2000.279.3.L429. View

18.

Boland G, Perkins G, Hall D, Tuan R . Wnt 3a promotes proliferation and suppresses osteogenic differentiation of adult human mesenchymal stem cells. J Cell Biochem. 2004; 93(6):1210-30. DOI: 10.1002/jcb.20284. View

19.

Chong J, Chandrakanthan V, Xaymardan M, Asli N, Li J, Ahmed I . Adult cardiac-resident MSC-like stem cells with a proepicardial origin. Cell Stem Cell. 2011; 9(6):527-40. PMC: 3652240. DOI: 10.1016/j.stem.2011.10.002. View

20.

Danielyan L, Schafer R, Schulz A, Ladewig T, Lourhmati A, Buadze M . Survival, neuron-like differentiation and functionality of mesenchymal stem cells in neurotoxic environment: the critical role of erythropoietin. Cell Death Differ. 2009; 16(12):1599-614. DOI: 10.1038/cdd.2009.95. View