A Mitochondria-dependent Pathway Mediates the Apoptosis of GSE-induced Yeast

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2012 Mar 10

PMID 22403727

Citations 10

Authors

Sishuo Cao

Wentao Xu

Nan Zhang

Yan Wang

Yunbo Luo

Xiaoyun He

Kunlun Huang

Affiliations

Soon will be listed here.

Abstract

Grapefruit seed extract (GSE), which has powerful anti-fungal activity, can induce apoptosis in S. cerevisiae. The yeast cells underwent apoptosis as determined by testing for apoptotic markers of DNA cleavage and typical chromatin condensation by Terminal Deoxynucleotidyl Transferase-mediated dUTP Nick End Labeling (TUNEL) and 4,6'-diaminidino-2-phenylindole (DAPI) staining and electron microscopy. The changes of ΔΨmt (mitochondrial transmembrane potential) and ROS (reactive oxygen species) indicated that the mitochondria took part in the apoptotic process. Changes in this process detected by metabonomics and proteomics revealed that the yeast cells tenaciously resisted adversity. Proteins related to redox, cellular structure, membrane, energy and DNA repair were significantly increased. In this study, the relative changes in the levels of proteins and metabolites showed the tenacious resistance of yeast cells. However, GSE induced apoptosis in the yeast cells by destruction of the mitochondrial 60 S ribosomal protein, L14-A, and prevented the conversion of pantothenic acid to coenzyme A (CoA). The relationship between the proteins and metabolites was analyzed by orthogonal projections to latent structures (OPLS). We found that the changes of the metabolites and the protein changes had relevant consistency.

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References

Pozniakovsky A, Knorre D, Markova O, Hyman A, Skulachev V, Severin F . Role of mitochondria in the pheromone- and amiodarone-induced programmed death of yeast. J Cell Biol. 2005; 168(2):257-69. PMC: 2171581. DOI: 10.1083/jcb.200408145. View

Pham N, Robinson B, Hedley D . Simultaneous detection of mitochondrial respiratory chain activity and reactive oxygen in digitonin-permeabilized cells using flow cytometry. Cytometry. 2000; 41(4):245-51. DOI: 10.1002/1097-0320(20001201)41:4<245::aid-cyto2>3.0.co;2-#. View

Bach B, Meudec E, Lepoutre J, Rossignol T, Blondin B, Dequin S . New insights into {gamma}-aminobutyric acid catabolism: Evidence for {gamma}-hydroxybutyric acid and polyhydroxybutyrate synthesis in Saccharomyces cerevisiae. Appl Environ Microbiol. 2009; 75(13):4231-9. PMC: 2704823. DOI: 10.1128/AEM.00051-09. View

Agarwal C, Tyagi A, Agarwal R . Gallic acid causes inactivating phosphorylation of cdc25A/cdc25C-cdc2 via ATM-Chk2 activation, leading to cell cycle arrest, and induces apoptosis in human prostate carcinoma DU145 cells. Mol Cancer Ther. 2006; 5(12):3294-302. DOI: 10.1158/1535-7163.MCT-06-0483. View

Andersen G, Andersen B, Dobritzsch D, Schnackerz K, Piskur J . A gene duplication led to specialized gamma-aminobutyrate and beta-alanine aminotransferase in yeast. FEBS J. 2007; 274(7):1804-17. DOI: 10.1111/j.1742-4658.2007.05729.x. View

Longo V, Mitteldorf J, Skulachev V . Programmed and altruistic ageing. Nat Rev Genet. 2005; 6(11):866-72. DOI: 10.1038/nrg1706. View

Almeida B, Ohlmeier S, Almeida A, Madeo F, Leao C, Rodrigues F . Yeast protein expression profile during acetic acid-induced apoptosis indicates causal involvement of the TOR pathway. Proteomics. 2009; 9(3):720-32. DOI: 10.1002/pmic.200700816. View

Sun W, Xing B, Sun Y, Du X, Lu M, Hao C . Proteome analysis of hepatocellular carcinoma by two-dimensional difference gel electrophoresis: novel protein markers in hepatocellular carcinoma tissues. Mol Cell Proteomics. 2007; 6(10):1798-808. DOI: 10.1074/mcp.M600449-MCP200. View

Singh L, Chen X, Kozich V, Kruger W . Chemical chaperone rescue of mutant human cystathionine beta-synthase. Mol Genet Metab. 2007; 91(4):335-42. PMC: 2040066. DOI: 10.1016/j.ymgme.2007.04.011. View

10.

Wilk S . Teaching resources. Apoptosis. Sci STKE. 2005; 2005(285):tr16. DOI: 10.1126/stke.2852005tr16. View

11.

Kluck R, Bossy-Wetzel E, Green D, Newmeyer D . The release of cytochrome c from mitochondria: a primary site for Bcl-2 regulation of apoptosis. Science. 1997; 275(5303):1132-6. DOI: 10.1126/science.275.5303.1132. View

12.

Mazzoni C, DAddario I, Falcone C . The C-terminus of the yeast Lsm4p is required for the association to P-bodies. FEBS Lett. 2007; 581(25):4836-40. DOI: 10.1016/j.febslet.2007.09.009. View

13.

Heggers J, Cottingham J, Gusman J, Reagor L, McCoy L, Carino E . The effectiveness of processed grapefruit-seed extract as an antibacterial agent: II. Mechanism of action and in vitro toxicity. J Altern Complement Med. 2002; 8(3):333-40. DOI: 10.1089/10755530260128023. View

14.

Reagor L, Gusman J, McCoy L, Carino E, Heggers J . The effectiveness of processed grapefruit-seed extract as an antibacterial agent: I. An in vitro agar assay. J Altern Complement Med. 2002; 8(3):325-32. DOI: 10.1089/10755530260128014. View

15.

Zhang X, Guo Y, Song Y, Sun W, Yu C, Zhao X . Proteomic analysis of individual variation in normal livers of human beings using difference gel electrophoresis. Proteomics. 2006; 6(19):5260-8. DOI: 10.1002/pmic.200600006. View

16.

Li M, Wang B, Zhang M, Rantalainen M, Wang S, Zhou H . Symbiotic gut microbes modulate human metabolic phenotypes. Proc Natl Acad Sci U S A. 2008; 105(6):2117-22. PMC: 2538887. DOI: 10.1073/pnas.0712038105. View

17.

Tapiero H, MATHE G, Couvreur P, Tew K . II. Glutamine and glutamate. Biomed Pharmacother. 2002; 56(9):446-57. DOI: 10.1016/s0753-3322(02)00285-8. View

18.

Liu X, Kim C, Yang J, Jemmerson R, Wang X . Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell. 1996; 86(1):147-57. DOI: 10.1016/s0092-8674(00)80085-9. View

19.

Kitagaki H, Araki Y, Funato K, Shimoi H . Ethanol-induced death in yeast exhibits features of apoptosis mediated by mitochondrial fission pathway. FEBS Lett. 2007; 581(16):2935-42. DOI: 10.1016/j.febslet.2007.05.048. View

20.

Raina K, Rajamanickam S, Deep G, Singh M, Agarwal R, Agarwal C . Chemopreventive effects of oral gallic acid feeding on tumor growth and progression in TRAMP mice. Mol Cancer Ther. 2008; 7(5):1258-67. DOI: 10.1158/1535-7163.MCT-07-2220. View