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Overcoming the Effects of Matrix Interference in the Measurement of Urine Protein Analytes

Overview
Journal Biomark Insights
Publisher Sage Publications
Date 2012 Mar 10
PMID 22403482
Citations 16
Authors
Timothy P Taylor
Michael G Janech
Elizabeth H Slate
Evelyn C Lewis
John M Arthur
Jim C Oates
Affiliations
Soon will be listed here.
Abstract

Using multiplex bead assays to measure urine proteins has a great potential for biomarker discovery, but substances in urine (the matrix) can interfere with assay measurements. By comparing the recovery of urine spiked with known quantities of several common analytes, this study demonstrated that the urine matrix variably interfered with the accurate measurement of low abundance proteins. Dilution of the urine permitted a more accurate measure of these proteins, equivalent to the standard dilution technique when the diluted analytes were above the limits of detection of the assay. Therefore, dilution can be used as an effective technique for over-coming urine matrix effects in urine immunoassays. These results may be applicable to other biological fluids in which matrix components interfere with assay performance.

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