LIS1-dependent Retrograde Translocation of Excitatory Synapses in Developing Interneuron Dendrites

Overview

Journal Nat Commun

Specialty Biology

Date 2012 Mar 8

PMID 22395613

Citations 9

Authors

Izumi Kawabata

Yutaro Kashiwagi

Kazuki Obashi

Masamichi Ohkura

Junichi Nakai

Anthony Wynshaw-Boris

Yuchio Yanagawa

Shigeo Okabe

Affiliations

Soon will be listed here.

Abstract

Synaptic remodelling coordinated with dendritic growth is essential for proper development of neural connections. After establishment of synaptic contacts, synaptic junctions are thought to become stationary and provide fixed anchoring points for further dendritic growth. However, the possibility of active translocation of synapses along dendritic protrusions, to guide the proper arrangement of synaptic distribution, has not yet been fully investigated. Here we show that immature dendrites of γ-aminobutyric acid-positive interneurons form long protrusions and that these protrusions serve as conduits for retrograde translocation of synaptic contacts to the parental dendrites. This translocation process is dependent on microtubules and the activity of LIS1, an essential regulator of dynein-mediated motility. Suppression of this retrograde translocation results in disorganized synaptic patterns on interneuron dendrites. Taken together, these findings suggest the existence of an active microtubule-dependent mechanism for synaptic translocation that helps in the establishment of proper synaptic distribution on dendrites.

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