Ex Vivo Evaluation of Insulin Nanoparticles Using Chitosan and Arabic Gum

Overview

Journal ISRN Pharm

Date 2012 Mar 6

PMID 22389865

Citations 6

Authors

M R Avadi

A M M Sadeghi

Naser Mohamadpour Dounighi

R Dinarvand

F Atyabi

M Rafiee-Tehrani

Affiliations

Soon will be listed here.

Abstract

Polymeric delivery systems based on nanoparticles have emerged as a promising approach for peroral insulin delivery. The aim of the present study was to investigate the release of insulin nanoparticulate systems and ex vivo studies. The nanoparticles were prepared by the ion gelation method. Particle size distribution, zeta potential, and polydispersity index of the nanoparticles were determined. It was found that the nanoparticles carried positive charges and showed a size distribution in the range of 170-200 nm. The electrostatic interactions between the positively charged group of chitosan and negatively charged groups of Arabic gum play an important role in the association efficiency of insulin in nanoparticles. In vitro insulin release studies showed an initial burst followed by a slow release of insulin. The mucoadhesion of the nanosystem was evaluated using excised rat jejunum. Ex vivo studies have shown a significant increase in absorption of insulin in the presence of chitosan nanoparticles in comparison with free insulin.

Citing Articles

Synthesis and Characterization of Environmentally Friendly Chitosan-Arabic Gum Nanoparticles for Encapsulation of Oregano Essential Oil in Pickering Emulsion.

Lobato-Guarnido I, Luzon G, Rios F, Fernandez-Serrano M Nanomaterials (Basel). 2023; 13(19).

PMID: 37836292 PMC: 10574744. DOI: 10.3390/nano13192651.

A novel 5-Fluorocuracil multiple-nanoemulsion used for the enhancement of oral bioavailability in the treatment of colorectal cancer.

Ahmad N, Albassam A, Khan M, Ullah Z, Mohammed Buheazah T, Salman AlHomoud H Saudi J Biol Sci. 2022; 29(5):3704-3716.

PMID: 35844373 PMC: 9280251. DOI: 10.1016/j.sjbs.2022.02.017.

Human Growth Hormone Fragment 176-191 Peptide Enhances the Toxicity of Doxorubicin-Loaded Chitosan Nanoparticles Against MCF-7 Breast Cancer Cells.

Habibullah M, Mohan S, Syed N, Makeen H, Jamal Q, Alothaid H Drug Des Devel Ther. 2022; 16:1963-1974.

PMID: 35783198 PMC: 9249349. DOI: 10.2147/DDDT.S367586.

Formulation development, and evaluation of chitosan engineered nanoparticles for ocular delivery of insulin.

Rathore P, Mahor A, Jain S, Haque A, Kesharwani P RSC Adv. 2022; 10(71):43629-43639.

PMID: 35519724 PMC: 9058365. DOI: 10.1039/d0ra07640f.

Toxicity Assessment and Control of Early Blight and Stem Rot of L. by Mancozeb-Loaded Chitosan-Gum Acacia Nanocomposites.

Kumar R, Duhan J, Manuja A, Kaur P, Kumar B, Sadh P J Xenobiot. 2022; 12(2):74-90.

PMID: 35466214 PMC: 9036208. DOI: 10.3390/jox12020008.

References

Bhardwaj T, Kanwar M, Lal R, Gupta A . Natural gums and modified natural gums as sustained-release carriers. Drug Dev Ind Pharm. 2000; 26(10):1025-38. DOI: 10.1081/ddc-100100266. View

Lowe P, Temple C . Calcitonin and insulin in isobutylcyanoacrylate nanocapsules: protection against proteases and effect on intestinal absorption in rats. J Pharm Pharmacol. 1994; 46(7):547-52. DOI: 10.1111/j.2042-7158.1994.tb03854.x. View

Owens D, Zinman B, Bolli G . Alternative routes of insulin delivery. Diabet Med. 2003; 20(11):886-98. DOI: 10.1046/j.1464-5491.2003.01076.x. View

Bayat A, Dorkoosh F, Dehpour A, Moezi L, Larijani B, Junginger H . Nanoparticles of quaternized chitosan derivatives as a carrier for colon delivery of insulin: ex vivo and in vivo studies. Int J Pharm. 2008; 356(1-2):259-66. DOI: 10.1016/j.ijpharm.2007.12.037. View

Illum L, Jabbal-Gill I, Hinchcliffe M, Fisher A, Davis S . Chitosan as a novel nasal delivery system for vaccines. Adv Drug Deliv Rev. 2001; 51(1-3):81-96. DOI: 10.1016/s0169-409x(01)00171-5. View

Wang X, Chi N, Tang X . Preparation of estradiol chitosan nanoparticles for improving nasal absorption and brain targeting. Eur J Pharm Biopharm. 2008; 70(3):735-40. DOI: 10.1016/j.ejpb.2008.07.005. View

Mao H, Roy K, Janes K, Lin K, Wang Y, August J . Chitosan-DNA nanoparticles as gene carriers: synthesis, characterization and transfection efficiency. J Control Release. 2001; 70(3):399-421. DOI: 10.1016/s0168-3659(00)00361-8. View

Galindo-Rodriguez S, Allemann E, Fessi H, Doelker E . Polymeric nanoparticles for oral delivery of drugs and vaccines: a critical evaluation of in vivo studies. Crit Rev Ther Drug Carrier Syst. 2005; 22(5):419-64. DOI: 10.1615/critrevtherdrugcarriersyst.v22.i5.10. View

Avadi M, Sadeghi A, Mohammadpour N, Abedin S, Atyabi F, Dinarvand R . Preparation and characterization of insulin nanoparticles using chitosan and Arabic gum with ionic gelation method. Nanomedicine. 2009; 6(1):58-63. DOI: 10.1016/j.nano.2009.04.007. View

10.

Mao S, Bakowsky U, Jintapattanakit A, Kissel T . Self-assembled polyelectrolyte nanocomplexes between chitosan derivatives and insulin. J Pharm Sci. 2006; 95(5):1035-48. DOI: 10.1002/jps.20520. View

11.

Takeuchi H, Yamamoto H, Kawashima Y . Mucoadhesive nanoparticulate systems for peptide drug delivery. Adv Drug Deliv Rev. 2001; 47(1):39-54. DOI: 10.1016/s0169-409x(00)00120-4. View

12.

Couvreur P, Barratt G, Fattal E, Legrand P, Vauthier C . Nanocapsule technology: a review. Crit Rev Ther Drug Carrier Syst. 2002; 19(2):99-134. DOI: 10.1615/critrevtherdrugcarriersyst.v19.i2.10. View

13.

Sandri G, Bonferoni M, Rossi S, Ferrari F, Gibin S, Zambito Y . Nanoparticles based on N-trimethylchitosan: evaluation of absorption properties using in vitro (Caco-2 cells) and ex vivo (excised rat jejunum) models. Eur J Pharm Biopharm. 2006; 65(1):68-77. DOI: 10.1016/j.ejpb.2006.07.016. View

14.

Barr W, RIEGELMAN S . Intestinal drug absorption and metabolism. I. Comparison of methods and models to study physiological factors of in vitro and in vivo intestinal absorption. J Pharm Sci. 1970; 59(2):154-63. DOI: 10.1002/jps.2600590204. View

15.

Janes K, Calvo P, Alonso M . Polysaccharide colloidal particles as delivery systems for macromolecules. Adv Drug Deliv Rev. 2001; 47(1):83-97. DOI: 10.1016/s0169-409x(00)00123-x. View