Comparative Studies of the (Anti) Mutagenicity of Baccharis Dracunculifolia and Artepillin C by the Bacterial Reverse Mutation Test

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2012 Feb 28

PMID 22367028

Citations 4

Authors

Flavia Aparecida Resende

Carla Carolina Munari

Moacir de Azevedo Bentes Monteiro Neto

Denise Crispim Tavares

Jairo Kenupp Bastos

Ademar Alves da Silva Filho

Eliana Aparecida Varanda

Affiliations

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Abstract

Baccharis dracunculifolia is a plant native from Brazil, commonly known as 'Alecrim-do-campo' and 'Vassoura' and used in alternative medicine for the treatment of inflammation, hepatic disorders and stomach ulcers. Previous studies reported that artepillin C (ArtC, 3-{4-hydroxy-3,5-di(3-methyl-2-butenyl)phenyl}-2(E)-propenoic acid), is the main compound of interest in the leaves. This study was undertaken to assess the mutagenic effect of the ethyl acetate extract of B. dracunculifolia leaves (Bd-EAE: 11.4-182.8 µg/plate) and ArtC (0.69-10.99 µg/plate) by the Ames test using Salmonella typhimurium strains TA98, TA97a, TA100 and TA102, and to compare the protective effects of Bd-EAE and ArtC against the mutagenicity of a variety of direct and indirect acting mutagens such as 4-nitro-O-phenylenediamine, sodium azide, mitomycin C, benzo[a]pyrene, aflatoxin B1, 2-aminoanthracene and 2-aminofluorene.The mutagenicity test showed that Bd-EAE and ArtC did not induce an increase in the number of revertant colonies indicating absence of mutagenic activity. ArtC showed a similar antimutagenic effect to that of Bd-EAE in some strains of S. typhimurium, demonstrating that the antimutagenic activity of Bd-EAE can be partially attributed to ArtC. The present results showed that the protective effect of whole plant extracts is due to the combined and synergistic effects of a complex mixture of phytochemicals, the total activity of which may result in health benefits.

Citing Articles

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Antimutagenic, antigenotoxic and antiproliferative activities of Fraxinus angustifolia Vahl. leaves and stem bark extracts and their phytochemical composition.

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References

Lemos M, Barros M, Sousa J, da Silva Filho A, Bastos J, de Andrade S . Baccharis dracunculifolia, the main botanical source of Brazilian green propolis, displays antiulcer activity. J Pharm Pharmacol. 2007; 59(4):603-8. DOI: 10.1211/jpp.59.4.0017. View

Bernstein L, Kaldor J, McCann J, Pike M . An empirical approach to the statistical analysis of mutagenesis data from the Salmonella test. Mutat Res. 1982; 97(4):267-81. DOI: 10.1016/0165-1161(82)90026-7. View

Lira W, dos Santos F, Sannomiya M, Rodrigues C, Vilegas W, Varanda E . Modulatory effect of Byrsonima basiloba extracts on the mutagenicity of certain direct and indirect-acting mutagens in Salmonella typhimurium assays. J Med Food. 2008; 11(1):111-9. DOI: 10.1089/jmf.2007.553. View

Quiles J, Huertas J, Battino M, Mataix J, Ramirez-Tortosa M . Antioxidant nutrients and adriamycin toxicity. Toxicology. 2002; 180(1):79-95. DOI: 10.1016/s0300-483x(02)00383-9. View

Messerli S, Ahn M, Kunimasa K, Yanagihara M, Tatefuji T, Hashimoto K . Artepillin C (ARC) in Brazilian green propolis selectively blocks oncogenic PAK1 signaling and suppresses the growth of NF tumors in mice. Phytother Res. 2008; 23(3):423-7. DOI: 10.1002/ptr.2658. View

Munari C, Alves J, Bastos J, Tavares D . Evaluation of the genotoxic and antigenotoxic potential of Baccharis dracunculifolia extract on V79 cells by the comet assay. J Appl Toxicol. 2009; 30(1):22-8. DOI: 10.1002/jat.1467. View

Munari C, Resende F, Alves J, de Sousa J, Bastos J, Tavares D . Mutagenicity and antimutagenicity of Baccharis dracunculifolia extract in chromosomal aberration assays in Chinese hamster ovary cells. Planta Med. 2008; 74(11):1363-7. DOI: 10.1055/s-2008-1081306. View

Cao P, Cai J, Gupta R . Effect of green tea catechins and hydrolyzable tannins on benzo[a]pyrene-induced DNA adducts and structure-activity relationship. Chem Res Toxicol. 2010; 23(4):771-7. PMC: 5087322. DOI: 10.1021/tx900412a. View

Resende F, Tomazella I, Barbosa L, Ponce M, Andrade Furtado R, Pereira A . Effect of the dibenzylbutyrolactone lignan (-)-hinokinin on doxorubicin and methyl methanesulfonate clastogenicity in V79 Chinese hamster lung fibroblasts. Mutat Res. 2010; 700(1-2):62-6. DOI: 10.1016/j.mrgentox.2010.04.023. View

10.

Park Y, Paredes-Guzman J, Aguiar C, Alencar S, Fujiwara F . Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian propolis. J Agric Food Chem. 2004; 52(5):1100-3. DOI: 10.1021/jf021060m. View

11.

Santos F, Colus I, Silva M, Vilegas W, Varanda E . Assessment of DNA damage by extracts and fractions of Strychnos pseudoquina, a Brazilian medicinal plant with antiulcerogenic activity. Food Chem Toxicol. 2006; 44(9):1585-9. DOI: 10.1016/j.fct.2006.03.012. View

12.

Paulino N, Lemos Abreu S, Uto Y, Koyama D, Nagasawa H, Hori H . Anti-inflammatory effects of a bioavailable compound, Artepillin C, in Brazilian propolis. Eur J Pharmacol. 2008; 587(1-3):296-301. DOI: 10.1016/j.ejphar.2008.02.067. View

13.

Xia Y, Cheng S, He J, Liu X, Tang Y, Yuan H . Effects of subchronic exposure to benzo[a]pyrene (B[a]P) on learning and memory, and neurotransmitters in male Sprague-Dawley rat. Neurotoxicology. 2011; 32(2):188-98. DOI: 10.1016/j.neuro.2010.12.015. View

14.

Shimizu K, Ashida H, Matsuura Y, Kanazawa K . Antioxidative bioavailability of artepillin C in Brazilian propolis. Arch Biochem Biophys. 2004; 424(2):181-8. DOI: 10.1016/j.abb.2004.02.021. View

15.

Resende F, Alves J, Munari C, Senedese J, Sousa J, Bastos J . Inhibition of doxorubicin-induced mutagenicity by Baccharis dracunculifolia. Mutat Res. 2007; 634(1-2):112-8. DOI: 10.1016/j.mrgentox.2007.06.008. View

16.

Pan M, Ghai G, Ho C . Food bioactives, apoptosis, and cancer. Mol Nutr Food Res. 2007; 52(1):43-52. DOI: 10.1002/mnfr.200700380. View

17.

Chen L, Devanesan P, Higginbotham S, Ariese F, Jankowiak R, Small G . Expanded analysis of benzo[a]pyrene-DNA adducts formed in vitro and in mouse skin: their significance in tumor initiation. Chem Res Toxicol. 1996; 9(5):897-903. DOI: 10.1021/tx960004a. View

18.

de Sousa J, da Silva Filho A, Bueno P, Gregorio L, Furtado N, Jorge R . A validated reverse-phase HPLC analytical method for the quantification of phenolic compounds in Baccharis dracunculifolia. Phytochem Anal. 2008; 20(1):24-32. DOI: 10.1002/pca.1087. View

19.

Schwarz D, Kisselev P, Cascorbi I, Schunck W, Roots I . Differential metabolism of benzo[a]pyrene and benzo[a]pyrene-7,8-dihydrodiol by human CYP1A1 variants. Carcinogenesis. 2001; 22(3):453-9. DOI: 10.1093/carcin/22.3.453. View

20.

Park Y, Alencar S, Aguiar C . Botanical origin and chemical composition of Brazilian propolis. J Agric Food Chem. 2002; 50(9):2502-6. DOI: 10.1021/jf011432b. View