Location of the Cis-acting Auxin-responsive Region in the Promoter of the Par Gene from Tobacco Mesophyll Protoplasts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1990 Oct 1

PMID 2236015

Citations 17

Authors

Y Takahashi

Y Niwa

Y Machida

T Nagata

Affiliations

Soon will be listed here.

Abstract

We have isolated a genomic clone of an auxin-regulated par gene, which is expressed during the transition from G0 phase to S phase in the early stage of tobacco mesophyll protoplasts cultured in vitro, from a tobacco genomic library using the par cDNA as a probe. When a chimeric gene, in which a reporter gene for bacterial beta-glucuronidase (GUS) was placed downstream of the 5' flanking sequences of the par gene, was introduced into tobacco mesophyll protoplasts by electroporation, the chimeric gene elicited auxin-regulated expression of GUS activity. Because deletion of a 111-base-pair (bp) direct repeat in the 5' flanking sequences of the par gene abolished the auxin-induced GUS activity, it is deduced that in the 111-bp direct repeat of the par gene promoter is localized an auxin-responsive region, which regulates auxin-mediated activation of transcription.

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