Genotypic and Phenotypic Changes of BHK-21 Cells Grown in Suspension Cultures

Overview

Journal Cytotechnology

Specialties Biotechnology
Genetics

Date 2012 Feb 24

PMID 22358669

Authors

M Amadori

C Berneri

Affiliations

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Abstract

The propagation of foot-and-mouth disease virus on BHK-21 suspension cells, although economically convenient, may yield a scarcely immunizing antigen. Helpful insights were obtained by investigating a few genotypic and phenotypic features of the cell cultures. The appearance of polyploid populations, higher cell concentrations at the end of culturing, the progressive reduction of spreading on surfaces and an abnormal expression of the α5β1 integrin were found to be correlated with the number of passages in suspension culture. The observed modifications in the normal course of the cell-cycle and in the expression of some surface proteins point at a possible mechanism of virus damages arising from defective cellular functions.

References

Arunachalam B, Talwar G, Raghupathy R . A simplified cellular ELISA (CELISA) for the detection of antibodies reacting with cell-surface antigens. J Immunol Methods. 1990; 135(1-2):181-9. DOI: 10.1016/0022-1759(90)90271-v. View

Grigera P, Tisminetzky S, Lebendiker M, Periolo O, La Torre J . Presence of a 43-kDa host-cell polypeptide in purified aphthovirions. Virology. 1988; 165(2):584-8. DOI: 10.1016/0042-6822(88)90602-2. View

Brown P, Juliano R . Monoclonal antibodies to distinctive epitopes on the alpha and beta subunits of the fibronectin receptor. Exp Cell Res. 1988; 177(2):303-18. DOI: 10.1016/0014-4827(88)90464-8. View

Montagnier L . Biological and biochemical criteria for the characterization of the transformed stage of BHK21 cells. Dev Biol Stand. 1979; 42:27-31. View

Murray A . Creative blocks: cell-cycle checkpoints and feedback controls. Nature. 1992; 359(6396):599-604. DOI: 10.1038/359599a0. View